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P45622

- HEM2_BRADU

UniProt

P45622 - HEM2_BRADU

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development.1 Publication

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.1 Publication

    Cofactori

    Magnesium.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei221 – 2211Schiff-base intermediate with substrateBy similarity
    Binding sitei231 – 2311Substrate 1By similarity
    Binding sitei244 – 2441Substrate 1By similarity
    Metal bindingi260 – 2601MagnesiumBy similarity
    Active sitei275 – 2751Schiff-base intermediate with substrateBy similarity
    Binding sitei301 – 3011Substrate 2By similarity
    Binding sitei340 – 3401Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-5074-MONOMER.
    RETL1328306-WGS:GSTH-1519-MONOMER.
    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:blr5037
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 353353Delta-aminolevulinic acid dehydratasePRO_0000140494Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi224911.blr5037.

    Structurei

    3D structure databases

    ProteinModelPortaliP45622.
    SMRiP45622. Positions 31-350.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    KOiK01698.
    OrthoDBiEOG6VXFCB.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45622-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIKYGRPIE LREVSRRDGA AASPALDLAI RPRRNRKAEW ARRMVRENVL    50
    TTDDLIWPLF LIDGNNKREQ IASMPGVERL SVDQAVREAE RAMKLTIPCI 100
    ALFPYTDPSL RDEEGSEACN PNNLVCQAVR AIKKEFPEIG VLCDVALDPF 150
    TSHGHDGLIA DGAILNDETV AVLVRQALVQ AEAGCDIIAP SDMMDGRVAA 200
    IREGLDQAGL IDVQIMAYAA KYASAFYGPF RDAIGSAKTL TGDKRTYQMD 250
    SANTDEALRE VELDISEGAD MVMVKPGMPY LDVVRRVKDT FAMPTFAYQV 300
    SGEYAMIAAA AGNGWLDGDR AMMESLLAFK RAGADGVLSY FAPKAAEKLR 350
    TQG 353
    Length:353
    Mass (Da):38,582
    Last modified:November 1, 1995 - v1
    Checksum:iF143FA4892ACF1F8
    GO

    Sequence cautioni

    The sequence BAC50302.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24386 Genomic DNA. Translation: AAA89067.1.
    BA000040 Genomic DNA. Translation: BAC50302.1. Different initiation.
    PIRiA49925.
    RefSeqiNP_771677.2. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC50302; BAC50302; BAC50302.
    GeneIDi1051848.
    KEGGibja:blr5037.
    PATRICi21193740. VBIBraJap65052_5115.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24386 Genomic DNA. Translation: AAA89067.1 .
    BA000040 Genomic DNA. Translation: BAC50302.1 . Different initiation.
    PIRi A49925.
    RefSeqi NP_771677.2. NC_004463.1.

    3D structure databases

    ProteinModelPortali P45622.
    SMRi P45622. Positions 31-350.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.blr5037.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC50302 ; BAC50302 ; BAC50302 .
    GeneIDi 1051848.
    KEGGi bja:blr5037.
    PATRICi 21193740. VBIBraJap65052_5115.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    KOi K01698.
    OrthoDBi EOG6VXFCB.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .
    BioCyci BJAP224911:GJEJ-5074-MONOMER.
    RETL1328306-WGS:GSTH-1519-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bradyrhizobium japonicum delta-aminolevulinic acid dehydratase is essential for symbiosis with soybean and contains a novel metal-binding domain."
      Chauhan S., O'Brian M.R.
      J. Bacteriol. 175:7222-7227(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, FUNCTION, COFACTOR.
      Strain: I110.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiHEM2_BRADU
    AccessioniPrimary (citable) accession number: P45622
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3