Reviewed, UniProtKB/Swiss-Prot P45622 (HEM2_BRAJA)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	blr5037

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for nodule development.
Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Magnesium.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Delta-aminolevulinic acid dehydratase		PRO_0000140494
Regions
	Region	143 – 161	19	Magnesium-binding By similarity
Sites
	Active site	275	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45622-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F143FA4892ACF1F8	FASTA	353	38,582
	10 20 30 40 50 60 MAIKYGRPIE LREVSRRDGA AASPALDLAI RPRRNRKAEW ARRMVRENVL TTDDLIWPLF 70 80 90 100 110 120 LIDGNNKREQ IASMPGVERL SVDQAVREAE RAMKLTIPCI ALFPYTDPSL RDEEGSEACN 130 140 150 160 170 180 PNNLVCQAVR AIKKEFPEIG VLCDVALDPF TSHGHDGLIA DGAILNDETV AVLVRQALVQ 190 200 210 220 230 240 AEAGCDIIAP SDMMDGRVAA IREGLDQAGL IDVQIMAYAA KYASAFYGPF RDAIGSAKTL 250 260 270 280 290 300 TGDKRTYQMD SANTDEALRE VELDISEGAD MVMVKPGMPY LDVVRRVKDT FAMPTFAYQV 310 320 330 340 350 SGEYAMIAAA AGNGWLDGDR AMMESLLAFK RAGADGVLSY FAPKAAEKLR TQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bradyrhizobium japonicum delta-aminolevulinic acid dehydratase is essential for symbiosis with soybean and contains a novel metal-binding domain." Chauhan S., O'Brian M.R. J. Bacteriol. 175:7222-7227(1993) [PubMed: 8226669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: I110.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L24386 Genomic DNA. Translation: AAA89067.1. BA000040 Genomic DNA. Translation: BAC50302.1. Different initiation.
PIR	A49925.
RefSeq	NP_771677.2.
3D structure databases
HSSP	HSSP built from PDB template 1GZG based on UniProtKB Q59643.
ModBase	Search...
Genome annotation databases
GeneID	1051848.
GenomeReviews	Gene locus blr5037 in contig BA000040_GR.
KEGG	bja:blr5037.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P45622.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR5037-MON.
BRENDA	4.2.1.24. 280.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
ProDom	PD002304. AlaD_dehydratase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_BRAJA

Accession

Primary (citable) accession number: P45622

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)