P45621 (GSA_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	GSA1
Synonyms:	GSA

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. Porphyrin biosynthesis; chlorophyll biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Strongly expressed in leaves of etiolated plantlets independently of light treatment and, to a much lesser extent, in leaves of mature plants.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 28	28	Chloroplast Potential
	Chain	29 – 466	438	Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic		PRO_0000001260
Amino acid modifications
	Modified residue	306	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45621 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5523012AE808DE72	FASTA	466	49,646
	10 20 30 40 50 60 MAVSAITGAR LTLGMSLSSS TRSRTVAMAV SIDPKTDNKL TLTKSEEAFA AAKELMPGGV 70 80 90 100 110 120 NSPVRAFKSV GGQPIVIDSV KGSRMWDIDG NEYIDYVGSW GPAIIGHADD QVLAALGETM 130 140 150 160 170 180 KKGTSFGAPC LLENTLAELV IDAVPSIEMV RFVNSGTEAC MGALRLARAY TGREKIIKFE 190 200 210 220 230 240 GCYHGHADPF LVKAGSGVAT LGLPDSPGVP KAATFETLTA PYNDTEAIEK LFEANKGEIA 250 260 270 280 290 300 AVFLEPVVGN AGFIVPKPDF HSFLRKITKE NNTLLVFDEV MTGFRLSYGG AQEYFGITPD 310 320 330 340 350 360 ITTLGKIIGG GLPVGAYGGR RDIMEKVAPA GPMYQAGTLS GNPLAMTAGI ETLQRIKEPG 370 380 390 400 410 420 TYEYLDKITG ELVEGIIEAG KRAGHAICGG HIRGMFGFFF TEGPVYNFAD AKKSDTAKFA 430 440 450 460 RFFWGMLAEG VYLAPSQFEA GFTSLAHTSD DIKKTIAAAE KVFREI

References

[1]	"Expression of the soybean (Glycine max) glutamate 1-semialdehyde aminotransferase gene in symbiotic root nodules." Sangwan I., O'Brian M.R. Plant Physiol. 102:829-834(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Root nodule.
[2]	"gsa1 is a universal tetrapyrrole synthesis gene in soybean and is regulated by a GAGA element." Frustaci J.M., Sangwan I., O'Brian M.R. J. Biol. Chem. 270:7387-7393(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Essex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12453 mRNA. Translation: AAA33968.1. U20260 Genomic DNA. Translation: AAC48996.1.
PIR	JQ2263.
RefSeq	NP_001238043.1. NM_001251114.1.
UniGene	Gma.1779.
3D structure databases
ProteinModelPortal	P45621.
SMR	P45621. Positions 44-462.
ModBase	Search...
Proteomic databases
ProMEX	P45621.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GLYMA06G00510.1; GLYMA06G00510.1; GLYMA06G00510.
GeneID	547795.
KEGG	gmx:547795.
Phylogenomic databases
KO	K01845.
Enzyme and pathway databases
UniPathway	UPA00251; UER00317. UPA00668.
Gene expression databases
Genevestigator	P45621.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_SOYBN

Accession

Primary (citable) accession number: P45621

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program