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Protein

Phosphocarrier protein HPr

Gene

ptsH

Organism
Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III).
P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbon catabolite repression (CCR), a mechanism that allows bacteria to coordinate and optimize the utilization of available carbon sources. P-Ser-HPr also plays a role in inducer exclusion, in which it probably interacts with several non-PTS permeases and inhibits their transport activity (By similarity).By similarity

Catalytic activityi

Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine.

Enzyme regulationi

Phosphorylation on Ser-46 inhibits the phosphoryl transfer from enzyme I to HPr.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei15 – 151Pros-phosphohistidine intermediate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciMCAP340047:GC0H-713-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphocarrier protein HPr (EC:2.7.11.-)
Alternative name(s):
Histidine-containing protein
Gene namesi
Name:ptsH
Ordered Locus Names:MCAP_0716
OrganismiMycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154)
Taxonomic identifieri340047 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000001928 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 8988Phosphocarrier protein HPrPRO_0000107862Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461Phosphoserine; by HPrK/PPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Structurei

Secondary structure

1
89
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75Combined sources
Helixi16 – 2611Combined sources
Beta strandi30 – 378Combined sources
Beta strandi40 – 434Combined sources
Helixi47 – 537Combined sources
Beta strandi60 – 678Combined sources
Helixi70 – 8314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PCHX-ray1.80A2-89[»]
ProteinModelPortaliP45611.
SMRiP45611. Positions 2-89.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45611.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8988HPrPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HPr family.Curated
Contains 1 HPr domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000278399.
KOiK11189.
OMAiFVQEANK.
OrthoDBiPOG091H03ED.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFSAIITD KVGLHARPAS VLAKEASKFS SNITIIANEK QGNLKSIMNV
60 70 80
MAMAIKTGTE ITIQADGNDA DQAIQAIKQT MIDTALIQG
Length:89
Mass (Da):9,418
Last modified:January 23, 2007 - v3
Checksum:i9E5CFF9278028F49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22432 Unassigned DNA. Translation: AAA16213.1.
CP000123 Genomic DNA. Translation: ABC01760.1.
PIRiA49683.
RefSeqiWP_011387560.1. NC_007633.1.

Genome annotation databases

EnsemblBacteriaiABC01760; ABC01760; MCAP_0716.
GeneIDi23778330.
KEGGimcp:MCAP_0716.
PATRICi20005909. VBIMycCap130493_0705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22432 Unassigned DNA. Translation: AAA16213.1.
CP000123 Genomic DNA. Translation: ABC01760.1.
PIRiA49683.
RefSeqiWP_011387560.1. NC_007633.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PCHX-ray1.80A2-89[»]
ProteinModelPortaliP45611.
SMRiP45611. Positions 2-89.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC01760; ABC01760; MCAP_0716.
GeneIDi23778330.
KEGGimcp:MCAP_0716.
PATRICi20005909. VBIMycCap130493_0705.

Phylogenomic databases

HOGENOMiHOG000278399.
KOiK11189.
OMAiFVQEANK.
OrthoDBiPOG091H03ED.

Enzyme and pathway databases

BioCyciMCAP340047:GC0H-713-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP45611.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTHP_MYCCT
AccessioniPrimary (citable) accession number: P45611
Secondary accession number(s): Q2SRD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.