Reviewed, UniProtKB/Swiss-Prot P45597 (PTFAX_XANCP)

Names and origin

Protein names

Recommended name:
    Multiphosphoryl transfer protein
      Short name=MTP
Including the following 3 domains:
    1- Recommended name:
            Phosphoenolpyruvate-protein phosphotransferase
              EC=2.7.3.9
        Alternative name(s):
            Phosphotransferase system enzyme I
    2- Recommended name:
            Phosphocarrier protein HPr
                Short name=Protein H
    3- Recommended name:
            Fructose-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system fructose-specific EIIA component
            EIII-Fru

Gene names

Name:	fruB
Ordered Locus Names:	XCC2370

Organism

Xanthomonas campestris pv. campestris [Complete proteome] [HAMAP]

Taxonomic identifier

340 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.
Subcellular location	Cytoplasm Potential.
Domain	The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Belongs to the PEP-utilizing enzyme family. Contains 1 HPr domain. Contains 1 PTS EIIA type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate-protein phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 838	838	Multiphosphoryl transfer protein		PRO_0000186514
Regions
	Domain	7 – 147	141	PTS EIIA type-2
	Domain	161 – 253	93	HPr
	Region	274 – 838	565	PTS EI
	Compositional bias	700 – 705	6	Poly-Ala
Sites
	Active site	67	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity
	Active site	175	1	Pros-phosphohistidine intermediate; for HPr activity By similarity
	Active site	460	1	Tele-phosphohistidine intermediate; for PTS EI activity By similarity
Experimental info
	Sequence conflict	115	1	L → V in CAA85482. Ref.1
	Sequence conflict	412	1	P → A in CAA85482. Ref.1
	Sequence conflict	505	1	L → Q in CAA85482. Ref.1
	Sequence conflict	561 – 562	2	EG → N in CAA85482. Ref.1
	Sequence conflict	586	1	Q → H in CAA85482. Ref.1
	Sequence conflict	593	1	R → Q in CAA85482. Ref.1
	Sequence conflict	596	1	D → N in CAA85482. Ref.1
	Sequence conflict	618	1	L → S in CAA85482. Ref.1
	Sequence conflict	634 – 635	2	LL → SS in CAA85482. Ref.1
	Sequence conflict	645	1	L → S in CAA85482. Ref.1
	Sequence conflict	680	1	R → A in CAA85482. Ref.1
	Sequence conflict	686	1	D → H in CAA85482. Ref.1
	Sequence conflict	741	1	D → S in CAA85482. Ref.1
	Sequence conflict	820	1	Q → H in CAA85482. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P45597-1 [UniParc]. Last modified July 11, 2002. Version 3. Checksum: 78C87FDBA2E4DF42	FASTA	838	88,747
	10 20 30 40 50 60 MSSPSIAPVT PDLVRLRATA RDKDDAIAQA AQLLVAAGCV APGYDASMRR REGLANTFLG 70 80 90 100 110 120 HGLAIPHGVG EDRHLVRRDG IAVLQLPEGV EWNPGQTTRL VVGIAAQSDT HITLLRRLTR 130 140 150 160 170 180 LIQDPAQLEA LFTTDDPAVI VAALTGDRAP DTSAAPATDL AERFEWTIAY PSGLHARPAT 190 200 210 220 230 240 RWAETARGFS ARAQVRAGDQ AADAKSLVGL LQLGLRAGDS ITVSAKGSDA PALLKRLRAV 250 260 270 280 290 300 MDSLTAQEKA DAERAAQRRA APVIGWTPPQ AQPAIVGIGA SPGVAIGIVH RLRAAQTEVA 310 320 330 340 350 360 DQPIGLGDGG VLLHDALTRT RQQLAAIQDD TQRRLGASDA AIFKAQAELL NDTDLITRTC 370 380 390 400 410 420 QLMVEGHGVA WSWHQAVEQI ASGLAALGNP VLAGRAADLR DVGRRVLAQL DPAAAGAGLT 430 440 450 460 470 480 DLPEQPCILL AGDLSPSDTA NLDTDCVLGL ATAQGGPTSH TAILSRTLGL PALVAAGGQL 490 500 510 520 530 540 LDIEDGVTAI IDGSSGRLYI NPSELDLDAA RTHIAEQQAI REREAAQRAL PAETTDGHHI 550 560 570 580 590 600 DIGANVNLPE QVAMALTQGA EGVGLMRTEF LFLERGSTPT EDEQYQTYLA MARALDGRPL 610 620 630 640 650 660 IVRALDIGGD KQVAHLELPH EENPFLGVRG ARLLLRRPDL LEPQLRALYR AAKDGARLSI 670 680 690 700 710 720 MFPMITSVPE LISLREICAR IRAELDAPEL PIGIMIEVPA AAAQADVLAR HADFFSIGTN 730 740 750 760 770 780 DLTQYVLAID RQNPELAAEA DSLHPAVLRM IRSTIDGARK HDRWVGVCGG LAGDPFGASL 790 800 810 820 830 LAGLGVQELS MTPNDIPAVK ARLRGRALSA LQQLAEQALQ CETAEQVRAL EAQREGQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fructose phosphotransferase system of Xanthomonas campestris pv. campestris: characterization of the fruB gene." de Crecy-Lagard V., Binet M., Danchin A. Microbiology 141:2253-2260(1995) [PubMed: 7496537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13951 / NCIB 11803 / NRRL B-1459.
[2]	"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.
[3]	"Fructose catabolism in Xanthomonas campestris pv. campestris. Sequence of the PTS operon, characterization of the fructose-specific enzymes." de Crecy-Lagard V., Bouvet O.M., Lejeune P., Danchin A. J. Biol. Chem. 266:18154-18161(1991) [PubMed: 1655739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 825-838. Strain: ATCC 13951 / NCIB 11803 / NRRL B-1459.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z37113 Genomic DNA. Translation: CAA85482.1. AE008922 Genomic DNA. Translation: AAM41648.1. M69242 Genomic DNA. Translation: AAA27600.1.
PIR	C40944. S51680.
RefSeq	NP_637724.1.
3D structure databases
HSSP	HSSP built from PDB template 1POH based on UniProtKB P07006.
ModBase	Search...
Genome annotation databases
GeneID	998532.
GenomeReviews	Gene locus XCC2370 in contig AE008922_GR.
KEGG	xcc:XCC2370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P45597.
OMA	GVCGELA.
Enzyme and pathway databases
BioCyc	XCAM190485:XCC2370-MON.
BRENDA	2.7.3.9. 281360.
Family and domain databases
InterPro	IPR008279. PEP_mobile. IPR018274. PEP_mobile_CS. IPR006318. PEP_P_trans. IPR000121. PEP_utilizers. IPR002178. PTS_EIIA_2. IPR001020. PTS_HPr_His_P_site. IPR005698. PTS_HPr_prot. IPR000032. PTS_HPr_prot-like. IPR002114. PTS_HPr_Ser_P_site. IPR008731. PTS_PEP_utilis_N. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit. G3DSA:3.30.1340.10. PTS_HPr_protein. 1 hit. G3DSA:1.10.274.10. PTS_PEP_utilis_N. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF05524. PEP-utilisers_N. 1 hit. PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. PF00381. PTS-HPr. 1 hit. PF00359. PTS_EIIA_2. 1 hit. [Graphical view]
PRINTS	PR00107. PHOSPHOCPHPR. PR01736. PHPHTRNFRASE.
ProDom	PD002238. HPr_protein. 1 hit. PD000940. PEP_utilizers. 1 hit. PD001689. PTS_EIIA_2. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01003. PTS_HPr_family. 1 hit. TIGR01417. PTS_I_fam. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit. PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51350. PTS_HPR_DOM. 1 hit. PS00369. PTS_HPR_HIS. 1 hit. PS00589. PTS_HPR_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFAX_XANCP

Accession

Primary (citable) accession number: P45597
Secondary accession number(s): Q03397

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 11, 2002
Last modified:	November 3, 2009
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)