P45591 (COF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cofilin-2

Alternative name(s):
Cofilin, muscle isoform

Gene names

Name:

Cfl2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Subcellular location	Nucleus matrix. Cytoplasm › cytoskeleton.
Tissue specificity	Predominantly expressed in skeletal muscle.
Post-translational modification	The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
Sequence similarities	Belongs to the actin-binding proteins ADF family. Contains 1 ADF-H domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Ligand	Actin-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament depolymerization Inferred from electronic annotation. Source: InterPro
Cellular_component	actin cytoskeleton Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nuclear matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 166	165	Cofilin-2		PRO_0000214908
Regions
	Domain	4 – 153	150	ADF-H
	Motif	30 – 34	5	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	3	1	Phosphoserine Ref.5
	Modified residue	6	1	Phosphothreonine Ref.5
	Modified residue	89	1	Phosphotyrosine Ref.3 Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P45591 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 48B6D7E5AE9FE1CC	FASTA	166	18,710
	10 20 30 40 50 60 MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI 70 80 90 100 110 120 GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS 130 140 150 160 KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGSVVVS LEGKPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel cofilin isoform that is predominantly expressed in mammalian skeletal muscle." Ono S., Minami N., Abe H., Obinata T. J. Biol. Chem. 269:15280-15286(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H. Tissue: Skeletal muscle.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-89, MASS SPECTROMETRY. Tissue: Mast cell.
[4]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-89, MASS SPECTROMETRY. Tissue: Brain.
[5]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3 AND THR-6, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L29468 mRNA. Translation: AAA37433.1. BC007138 mRNA. Translation: AAH07138.1.
IPI	IPI00266188.
PIR	A53812.
RefSeq	NP_031714.1. NM_007688.2.
UniGene	Mm.276826.
3D structure databases
ProteinModelPortal	P45591.
SMR	P45591. Positions 1-166.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000077262.
PTM databases
PhosphoSite	P45591.
2D gel databases
REPRODUCTION-2DPAGE	P45591.
Proteomic databases
PaxDb	P45591.
PRIDE	P45591.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000078124; ENSMUSP00000077262; ENSMUSG00000062929.
GeneID	12632.
KEGG	mmu:12632.
Organism-specific databases
CTD	1073.
MGI	MGI:101763. Cfl2.
Phylogenomic databases
eggNOG	NOG303866.
HOGENOM	HOG000039697.
HOVERGEN	HBG000381.
InParanoid	P45591.
KO	K05765.
OMA	GLYDATY.
OrthoDB	EOG4WSWBP.
Gene expression databases
ArrayExpress	P45591.
Bgee	P45591.
CleanEx	MM_CFL2.
Genevestigator	P45591.
GermOnline	ENSMUSG00000062929. Mus musculus.
Family and domain databases
InterPro	IPR002108. Actin-bd_cofilin/tropomyosin. IPR017904. ADF/Cofilin/Destrin. IPR027234. Cofilin_1/2. [Graphical view]
PANTHER	PTHR11913. PTHR11913. 1 hit. PTHR11913:SF2. PTHR11913:SF2. 1 hit.
Pfam	PF00241. Cofilin_ADF. 1 hit. [Graphical view]
PRINTS	PR00006. COFILIN.
SMART	SM00102. ADF. 1 hit. [Graphical view]
PROSITE	PS51263. ADF_H. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CFL2. mouse.
NextBio	281820.
SOURCE	Search...

Entry information

Entry name

COF2_MOUSE

Accession

Primary (citable) accession number: P45591

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program