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P45582 (BGAL_ASPOF) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Lactase
EC=3.2.1.23
OrganismAsparagus officinalis (Garden asparagus)
Taxonomic identifier4686 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesAsparagaceaeAsparagoideaeAsparagus

Protein attributes

Sequence length832 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 832807Beta-galactosidase
PRO_0000012193

Regions

Domain741 – 83292SUEL-type lectin

Sites

Active site1831Proton donor Potential
Active site2521Nucleophile Potential

Sequences

Sequence LengthMass (Da)Tools
P45582 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 94ABDC61EC4164AE

FASTA83292,214
        10         20         30         40         50         60 
MALKLVLMLM VALLAAVWSP PAVTASVTYD HKSVIINGQR RILISGSIHY PRSTPEMWPD 

        70         80         90        100        110        120 
LIQKAKDGGL DVIQTYVFWN GHEPSPGQYY FGGRYDLVRF LKLVKQAGLY AHLRIGPYVC 

       130        140        150        160        170        180 
AEWNFGGFPV WLKYVPGIHF RTDNGPFKAA MGKFTEKIVS MMKAEGLYET QGGPIILSQI 

       190        200        210        220        230        240 
ENEYGPVEYY DGAAGKSYTN WAAKMAVGLN TGVPWVMCKQ DDAPDPVINT CNGFYCDYFS 

       250        260        270        280        290        300 
PNKDNKPKMW TEAWTGWFTG FGGAVPQRPA EDMAFAVARF IQKGGSFINY YMYHGGTNFG 

       310        320        330        340        350        360 
RTAGGPFIST SYDYDAPIDE YGLLRQPKWG HLRDLHKAIK LCEPALVSGE PTITSLGQNQ 

       370        380        390        400        410        420 
ESYVYRSKSS CAAFLANFNS RYYATVTFNG MHYNLPPWSV SILPDCKTTV FNTARVGAQT 

       430        440        450        460        470        480 
TTMKMQYLGG FSWKAYTEDT DALNDNTFTK DGLVEQLSTT WDRSDYLWYT TYVDIAKNEE 

       490        500        510        520        530        540 
FLKTGKYPYL TVMSAGHAVH VFINGQLSGT AYGSLDNPKL TYSGSAKLWA GSNKISILSV 

       550        560        570        580        590        600 
SVGLPNVGNH FETWNTGVLG PVTLTGLNEG KRDLSLQKWT YQIGLHGETL SLHSLTGSSN 

       610        620        630        640        650        660 
VEWGEASQKQ PLTWYKTFFN APPGNEPLAL DMNTMGKGQI WINGQSIGRY WPAYKASGSC 

       670        680        690        700        710        720 
GSCDYRGTYN EKKCLSNCGE ASQRWYHVPR SWLIPTGNFL VVLEEWGGDP TGISMVKRSV 

       730        740        750        760        770        780 
ASVCAEVEEL QPTMDNWRTK AYGRPKVHLS CDPGQKMSKI KFASFGTPQG TCGSFSEGSC 

       790        800        810        820        830 
HAHKSYDAFE QEGLMQNCVG QEFCSVNVAP EVFGGDPCPG TMKKLAVEAI CE 

« Hide

References

[1]"Cloning of a harvest-induced beta-galactosidase from tips of harvested asparagus spears."
King G.A., Davies K.M.
Plant Physiol. 108:419-420(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Limbras 10.
Tissue: Spear.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77319 mRNA. Translation: CAA54525.1.
PIRS41889.

3D structure databases

ProteinModelPortalP45582.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_ASPOF
AccessionPrimary (citable) accession number: P45582
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 16, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries