Reviewed, UniProtKB/Swiss-Prot P45582 (BGAL_ASPOF)

Names and origin

Protein names	Recommended name: Beta-galactosidase Short name=Lactase EC=3.2.1.23
Organism	Asparagus officinalis (Garden asparagus)
Taxonomic identifier	4686 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Asparagales › Asparagaceae › Asparagoideae › Asparagus

Protein attributes

Sequence length	832 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 832	807	Beta-galactosidase		PRO_0000012193
Regions
	Domain	741 – 832	92	SUEL-type lectin
Sites
	Active site	183	1	Proton donor Potential
	Active site	252	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	P45582-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 94ABDC61EC4164AE	FASTA	832	92,214
	10 20 30 40 50 60 MALKLVLMLM VALLAAVWSP PAVTASVTYD HKSVIINGQR RILISGSIHY PRSTPEMWPD 70 80 90 100 110 120 LIQKAKDGGL DVIQTYVFWN GHEPSPGQYY FGGRYDLVRF LKLVKQAGLY AHLRIGPYVC 130 140 150 160 170 180 AEWNFGGFPV WLKYVPGIHF RTDNGPFKAA MGKFTEKIVS MMKAEGLYET QGGPIILSQI 190 200 210 220 230 240 ENEYGPVEYY DGAAGKSYTN WAAKMAVGLN TGVPWVMCKQ DDAPDPVINT CNGFYCDYFS 250 260 270 280 290 300 PNKDNKPKMW TEAWTGWFTG FGGAVPQRPA EDMAFAVARF IQKGGSFINY YMYHGGTNFG 310 320 330 340 350 360 RTAGGPFIST SYDYDAPIDE YGLLRQPKWG HLRDLHKAIK LCEPALVSGE PTITSLGQNQ 370 380 390 400 410 420 ESYVYRSKSS CAAFLANFNS RYYATVTFNG MHYNLPPWSV SILPDCKTTV FNTARVGAQT 430 440 450 460 470 480 TTMKMQYLGG FSWKAYTEDT DALNDNTFTK DGLVEQLSTT WDRSDYLWYT TYVDIAKNEE 490 500 510 520 530 540 FLKTGKYPYL TVMSAGHAVH VFINGQLSGT AYGSLDNPKL TYSGSAKLWA GSNKISILSV 550 560 570 580 590 600 SVGLPNVGNH FETWNTGVLG PVTLTGLNEG KRDLSLQKWT YQIGLHGETL SLHSLTGSSN 610 620 630 640 650 660 VEWGEASQKQ PLTWYKTFFN APPGNEPLAL DMNTMGKGQI WINGQSIGRY WPAYKASGSC 670 680 690 700 710 720 GSCDYRGTYN EKKCLSNCGE ASQRWYHVPR SWLIPTGNFL VVLEEWGGDP TGISMVKRSV 730 740 750 760 770 780 ASVCAEVEEL QPTMDNWRTK AYGRPKVHLS CDPGQKMSKI KFASFGTPQG TCGSFSEGSC 790 800 810 820 830 HAHKSYDAFE QEGLMQNCVG QEFCSVNVAP EVFGGDPCPG TMKKLAVEAI CE

References

[1]	"Cloning of a harvest-induced beta-galactosidase from tips of harvested asparagus spears." King G.A., Davies K.M. Plant Physiol. 108:419-420(1995) [PubMed: 7784512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Limbras 10. Tissue: Spear.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77319 mRNA. Translation: CAA54525.1.
PIR	S41889.
3D structure databases
SMR	P45582. Positions 743-831.
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Enzyme and pathway databases
BRENDA	3.2.1.23. 129.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR000922. Lectin_gal_bd. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_ASPOF

Accession

Primary (citable) accession number: P45582

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	March 2, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)