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Protein

Beta-galactosidase

Gene
N/A
Organism
Asparagus officinalis (Garden asparagus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei183 – 1831Proton donorSequence Analysis
Active sitei252 – 2521NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Lactase
OrganismiAsparagus officinalis (Garden asparagus)
Taxonomic identifieri4686 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesAsparagaceaeAsparagoideaeAsparagus

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 832807Beta-galactosidasePRO_0000012193Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini741 – 83292SUEL-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKLVLMLM VALLAAVWSP PAVTASVTYD HKSVIINGQR RILISGSIHY
60 70 80 90 100
PRSTPEMWPD LIQKAKDGGL DVIQTYVFWN GHEPSPGQYY FGGRYDLVRF
110 120 130 140 150
LKLVKQAGLY AHLRIGPYVC AEWNFGGFPV WLKYVPGIHF RTDNGPFKAA
160 170 180 190 200
MGKFTEKIVS MMKAEGLYET QGGPIILSQI ENEYGPVEYY DGAAGKSYTN
210 220 230 240 250
WAAKMAVGLN TGVPWVMCKQ DDAPDPVINT CNGFYCDYFS PNKDNKPKMW
260 270 280 290 300
TEAWTGWFTG FGGAVPQRPA EDMAFAVARF IQKGGSFINY YMYHGGTNFG
310 320 330 340 350
RTAGGPFIST SYDYDAPIDE YGLLRQPKWG HLRDLHKAIK LCEPALVSGE
360 370 380 390 400
PTITSLGQNQ ESYVYRSKSS CAAFLANFNS RYYATVTFNG MHYNLPPWSV
410 420 430 440 450
SILPDCKTTV FNTARVGAQT TTMKMQYLGG FSWKAYTEDT DALNDNTFTK
460 470 480 490 500
DGLVEQLSTT WDRSDYLWYT TYVDIAKNEE FLKTGKYPYL TVMSAGHAVH
510 520 530 540 550
VFINGQLSGT AYGSLDNPKL TYSGSAKLWA GSNKISILSV SVGLPNVGNH
560 570 580 590 600
FETWNTGVLG PVTLTGLNEG KRDLSLQKWT YQIGLHGETL SLHSLTGSSN
610 620 630 640 650
VEWGEASQKQ PLTWYKTFFN APPGNEPLAL DMNTMGKGQI WINGQSIGRY
660 670 680 690 700
WPAYKASGSC GSCDYRGTYN EKKCLSNCGE ASQRWYHVPR SWLIPTGNFL
710 720 730 740 750
VVLEEWGGDP TGISMVKRSV ASVCAEVEEL QPTMDNWRTK AYGRPKVHLS
760 770 780 790 800
CDPGQKMSKI KFASFGTPQG TCGSFSEGSC HAHKSYDAFE QEGLMQNCVG
810 820 830
QEFCSVNVAP EVFGGDPCPG TMKKLAVEAI CE
Length:832
Mass (Da):92,214
Last modified:October 31, 1995 - v1
Checksum:i94ABDC61EC4164AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77319 mRNA. Translation: CAA54525.1.
PIRiS41889.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77319 mRNA. Translation: CAA54525.1.
PIRiS41889.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of a harvest-induced beta-galactosidase from tips of harvested asparagus spears."
    King G.A., Davies K.M.
    Plant Physiol. 108:419-420(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Limbras 10.
    Tissue: Spear.

Entry informationi

Entry nameiBGAL_ASPOF
AccessioniPrimary (citable) accession number: P45582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: October 31, 1995
Last modified: March 31, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.