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Protein

Hydroxycarboxylate dehydrogenase A

Gene

hcxA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 2-oxoglutarate and 2-oxobutanoate, leading to the respective 2-hydroxycarboxylate. Cannot use NADH instead of NADPH as a redox partner. Do not catalyze the reverse reactions.1 Publication

Catalytic activityi

2-oxobutanoate + NADPH = 2-hydroxybutanoate + NADP+.1 Publication
2-oxoglutarate + NADPH = 2-hydroxyglutarate + NADP+.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi173Zinc; catalyticBy similarity1
Metal bindingi257Zinc; catalyticBy similarity1
Metal bindingi274Zinc; catalyticBy similarity1

GO - Molecular functioni

  • 2-hydroxyglutarate dehydrogenase activity Source: EcoCyc
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NADP, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG12692-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxycarboxylate dehydrogenase A1 Publication (EC:1.1.1.-1 Publication)
Alternative name(s):
2-oxobutanoate reductase1 Publication
2-oxoglutarate reductase1 Publication
Gene namesi
Name:hcxA1 Publication
Synonyms:ybdHImported
Ordered Locus Names:b0599, JW0592
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12692. ybdH.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show a consistent change in 2-hydroxyglutarate level.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878311 – 362Hydroxycarboxylate dehydrogenase AAdd BLAST362

Proteomic databases

PaxDbiP45579.
PRIDEiP45579.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259892. 10 interactors.
DIPiDIP-11347N.
IntActiP45579. 11 interactors.
MINTiMINT-1302765.
STRINGi316385.ECDH10B_0668.

Structurei

3D structure databases

ProteinModelPortaliP45579.
SMRiP45579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107SCQ. Bacteria.
COG0371. LUCA.
HOGENOMiHOG000031783.
InParanoidiP45579.
KOiK08317.
PhylomeDBiP45579.

Family and domain databases

InterProiView protein in InterPro
IPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
PfamiView protein in Pfam
PF00465. Fe-ADH. 1 hit.
PROSITEiView protein in PROSITE
PS00913. ADH_IRON_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P45579-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHNPIRVVV GPANYFSHPG SFNHLHDFFT DEQLSRAVWI YGKRAIAAAQ
60 70 80 90 100
TKLPPAFGLP GAKHILFRGH CSESDVQQLA AESGDDRSVV IGVGGGALLD
110 120 130 140 150
TAKALARRLG LPFVAVPTIA ATCAAWTPLS VWYNDAGQAL HYEIFDDANF
160 170 180 190 200
MVLVEPEIIL NAPQQYLLAG IGDTLAKWYE AVVLAPQPET LPLTVRLGIN
210 220 230 240 250
NAQAIRDVLL NSSEQALSDQ QNQQLTQSFC DVVDAIIAGG GMVGGLGDRF
260 270 280 290 300
TRVAAAHAVH NGLTVLPQTE KFLHGTKVAY GILVQSALLG QDDVLAQLTG
310 320 330 340 350
AYQRFHLPTT LAELEVDINN QAEIDKVIAH TLRPVESIHY LPVTLTPDTL
360
RAAFKKVESF KA
Length:362
Mass (Da):39,092
Last modified:November 1, 1997 - v2
Checksum:iC28BBB4F8F247AE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40800.1.
U00096 Genomic DNA. Translation: AAC73700.1.
AP009048 Genomic DNA. Translation: BAA35229.1.
X52904 Genomic DNA. No translation available.
PIRiE64793.
RefSeqiNP_415132.1. NC_000913.3.
WP_001120441.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73700; AAC73700; b0599.
BAA35229; BAA35229; BAA35229.
GeneIDi945212.
KEGGiecj:JW0592.
eco:b0599.
PATRICifig|1411691.4.peg.1669.

Similar proteinsi

Entry informationi

Entry nameiHCXA_ECOLI
AccessioniPrimary (citable) accession number: P45579
Secondary accession number(s): P77632
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: August 30, 2017
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families