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P45578 (LUXS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-ribosylhomocysteine lyase

EC=4.4.1.21
Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS
Gene names
Name:luxS
Synonyms:ygaG
Ordered Locus Names:b2687, JW2662
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length171 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). Ref.8 Ref.9

Catalytic activity

S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP-Rule MF_00091

Cofactor

Binds 1 iron ion per subunit By similarity. HAMAP-Rule MF_00091

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00091

Miscellaneous

E.coli strain DH5-alpha does not make AI-2; it has a frameshift mutation in the luxS gene that disrupts the protein coding region.

Sequence similarities

Belongs to the LuxS family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

dnaKP0A6Y81EBI-562313,EBI-542092

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 171170S-ribosylhomocysteine lyase HAMAP-Rule MF_00091
PRO_0000172220

Sites

Metal binding541Iron By similarity
Metal binding581Iron By similarity
Metal binding1281Iron By similarity

Experimental info

Sequence conflict311D → E in AAB40286. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P45578 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 131F57F1866DA105

FASTA17119,416
        10         20         30         40         50         60 
MPLLDSFTVD HTRMEAPAVR VAKTMNTPHG DAITVFDLRF CVPNKEVMPE RGIHTLEHLF 

        70         80         90        100        110        120 
AGFMRNHLNG NGVEIIDISP MGCRTGFYMS LIGTPDEQRV ADAWKAAMED VLKVQDQNQI 

       130        140        150        160        170 
PELNVYQCGT YQMHSLQEAQ DIARSILERD VRINSNEELA LPKEKLQELH I 

« Hide

References

« Hide 'large scale' references
[1]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-76.
Strain: K12 / MG1655 / ATCC 47076.
[5]"Emr, an Escherichia coli locus for multidrug resistance."
Lomovskaya O., Lewis K.
Proc. Natl. Acad. Sci. U.S.A. 89:8938-8942(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-171.
[6]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-10.
Strain: K12 / EMG2.
[7]"Detection of new genes in a bacterial genome using Markov models for three gene classes."
Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.
Nucleic Acids Res. 23:3554-3562(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[8]"Quorum sensing in Escherichia coli and Salmonella typhimurium."
Surette M.G., Bassler B.L.
Proc. Natl. Acad. Sci. U.S.A. 95:7046-7050(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production."
Surette M.G., Miller M.B., Bassler B.L.
Proc. Natl. Acad. Sci. U.S.A. 96:1639-1644(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC75734.1.
AP009048 Genomic DNA. Translation: BAA16549.1.
U83186 Genomic DNA. Translation: AAB40286.1.
M86657 Genomic DNA. No translation available.
PIRH65048.
RefSeqNP_417172.1. NC_000913.3.
YP_490901.1. NC_007779.1.

3D structure databases

ProteinModelPortalP45578.
SMRP45578. Positions 3-161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10131N.
IntActP45578. 5 interactions.
STRING511145.b2687.

Chemistry

BindingDBP45578.
ChEMBLCHEMBL3496.

2D gel databases

SWISS-2DPAGEP45578.

Proteomic databases

PaxDbP45578.
PRIDEP45578.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75734; AAC75734; b2687.
BAA16549; BAA16549; BAA16549.
GeneID12934186.
947168.
KEGGecj:Y75_p2630.
eco:b2687.
PATRIC32120768. VBIEscCol129921_2781.

Organism-specific databases

EchoBASEEB2573.
EcoGeneEG12712. luxS.

Phylogenomic databases

eggNOGCOG1854.
HOGENOMHOG000040371.
KOK07173.
OMARDHLNSD.
OrthoDBEOG68WRBM.
PhylomeDBP45578.

Enzyme and pathway databases

BioCycEcoCyc:EG12712-MONOMER.
ECOL316407:JW2662-MONOMER.
MetaCyc:EG12712-MONOMER.
BRENDA4.4.1.21. 2026.

Gene expression databases

GenevestigatorP45578.

Family and domain databases

Gene3D3.30.1360.80. 1 hit.
HAMAPMF_00091. LuxS.
InterProIPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
[Graphical view]
PfamPF02664. LuxS. 1 hit.
[Graphical view]
PIRSFPIRSF006160. AI2. 1 hit.
PRINTSPR01487. LUXSPROTEIN.
ProDomPD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF63411. SSF63411. 1 hit.
ProtoNetSearch...

Other

PROP45578.

Entry information

Entry nameLUXS_ECOLI
AccessionPrimary (citable) accession number: P45578
Secondary accession number(s): P77134, P77805
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene