Reviewed, UniProtKB/Swiss-Prot P45578 (LUXS_ECOLI)

Names and origin

Protein names

Recommended name:
    S-ribosylhomocysteine lyase
    EC=4.4.1.21
Alternative name(s):
    Autoinducer-2 production protein luxS
    AI-2 synthesis protein

Gene names

Name:	luxS
Synonyms:	ygaG
Ordered Locus Names:	b2687, JW2662

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). Ref.8 Ref.9
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091
Subunit structure	Homodimer By similarity. HAMAP MF_00091
Miscellaneous	E.coli strain DH5-alpha does not make AI-2; it has a frameshift mutation in the luxS gene that disrupts the protein coding region. HAMAP MF_00091
Sequence similarities	Belongs to the luxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	L-methionine biosynthetic process from S-adenosylmethionine Inferred from direct assay. Source: UniProtKB cell-cell signaling involved in quorum sensing Inferred from mutant phenotype. Source: UniProtKB generation of a signal involved in cell-cell signaling Inferred from direct assay. Source: UniProtKB
Molecular function	S-ribosylhomocysteine lyase activity Inferred from direct assay. Source: UniProtKB iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaK	P0A6Y8	1	EBI-562313,EBI-542092

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 171	170	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_0000172220
Sites
	Metal binding	54	1	Iron By similarity
	Metal binding	58	1	Iron By similarity
	Metal binding	128	1	Iron By similarity
Experimental info
	Sequence conflict	31	1	D → E in AAB40286. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P45578-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 131F57F1866DA105	FASTA	171	19,416
	10 20 30 40 50 60 MPLLDSFTVD HTRMEAPAVR VAKTMNTPHG DAITVFDLRF CVPNKEVMPE RGIHTLEHLF 70 80 90 100 110 120 AGFMRNHLNG NGVEIIDISP MGCRTGFYMS LIGTPDEQRV ADAWKAAMED VLKVQDQNQI 130 140 150 160 170 PELNVYQCGT YQMHSLQEAQ DIARSILERD VRINSNEELA LPKEKLQELH I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-76. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Emr, an Escherichia coli locus for multidrug resistance." Lomovskaya O., Lewis K. Proc. Natl. Acad. Sci. U.S.A. 89:8938-8942(1992) [PubMed: 1409590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-171.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-10. Strain: K12 / EMG2.
[7]	"Detection of new genes in a bacterial genome using Markov models for three gene classes." Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A. Nucleic Acids Res. 23:3554-3562(1995) [PubMed: 7567469] [Abstract] Cited for: IDENTIFICATION.
[8]	"Quorum sensing in Escherichia coli and Salmonella typhimurium." Surette M.G., Bassler B.L. Proc. Natl. Acad. Sci. U.S.A. 95:7046-7050(1998) [PubMed: 9618536] [Abstract] Cited for: FUNCTION.
[9]	"Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production." Surette M.G., Miller M.B., Bassler B.L. Proc. Natl. Acad. Sci. U.S.A. 96:1639-1644(1999) [PubMed: 9990077] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC75734.1. AP009048 Genomic DNA. Translation: BAA16549.1. U83186 Genomic DNA. Translation: AAB40286.1. M86657 Genomic DNA. No translation available.
PIR	H65048.
RefSeq	AP_003259.1. NP_417172.1.
3D structure databases
SMR	P45578. Positions 3-161.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10131N.
IntAct	P45578. 4 interactions.
STRING	P45578.
2-D gel databases
SWISS-2DPAGE	P45578.
2DBase-Ecoli	P45578.
Genome annotation databases
GeneID	947168.
GenomeReviews	Gene locus JW2662 in contig AP009048_GR. Gene locus b2687 in contig U00096_GR.
KEGG	ecj:JW2662. eco:b2687.
Organism-specific databases
EchoBASE	EB2573.
EcoGene	EG12712. luxS.
CMR	Search...
Phylogenomic databases
eggNOG	COG1854.
HOGENOM	HBG347473.
OMA	VRIAKTM.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12712-MONOMER. ECOL168927:B2687-MONOMER. MetaCyc:EG12712-MONOMER.
Gene expression databases
Genevestigator	P45578.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

LUXS_ECOLI

Accession

Primary (citable) accession number: P45578
Secondary accession number(s): P77134, P77805

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)