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P45578

- LUXS_ECOLI

UniProt

P45578 - LUXS_ECOLI

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Protein

S-ribosylhomocysteine lyase

Gene

luxS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).2 Publications

Catalytic activityi

S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione.

Cofactori

Binds 1 iron ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541IronBy similarity
Metal bindingi58 – 581IronBy similarity
Metal bindingi128 – 1281IronBy similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. S-ribosylhomocysteine lyase activity Source: EcoCyc

GO - Biological processi

  1. cell-cell signaling involved in quorum sensing Source: EcoCyc
  2. L-methionine biosynthetic process from S-adenosylmethionine Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Autoinducer synthesis, Quorum sensing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12712-MONOMER.
ECOL316407:JW2662-MONOMER.
MetaCyc:EG12712-MONOMER.
BRENDAi4.4.1.21. 2026.
SABIO-RKP45578.

Names & Taxonomyi

Protein namesi
Recommended name:
S-ribosylhomocysteine lyase (EC:4.4.1.21)
Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS
Gene namesi
Name:luxS
Synonyms:ygaG
Ordered Locus Names:b2687, JW2662
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12712. luxS.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 171170S-ribosylhomocysteine lyasePRO_0000172220Add
BLAST

Proteomic databases

PaxDbiP45578.
PRIDEiP45578.

2D gel databases

SWISS-2DPAGEP45578.

Expressioni

Gene expression databases

GenevestigatoriP45578.

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaKP0A6Y81EBI-562313,EBI-542092

Protein-protein interaction databases

DIPiDIP-10131N.
IntActiP45578. 5 interactions.
STRINGi511145.b2687.

Structurei

3D structure databases

ProteinModelPortaliP45578.
SMRiP45578. Positions 3-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LuxS family.Curated

Phylogenomic databases

eggNOGiCOG1854.
HOGENOMiHOG000040371.
InParanoidiP45578.
KOiK07173.
OMAiRDHLNSD.
OrthoDBiEOG68WRBM.
PhylomeDBiP45578.

Family and domain databases

Gene3Di3.30.1360.80. 1 hit.
HAMAPiMF_00091. LuxS.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
[Graphical view]
PfamiPF02664. LuxS. 1 hit.
[Graphical view]
PIRSFiPIRSF006160. AI2. 1 hit.
PRINTSiPR01487. LUXSPROTEIN.
ProDomiPD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF63411. SSF63411. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45578-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLLDSFTVD HTRMEAPAVR VAKTMNTPHG DAITVFDLRF CVPNKEVMPE
60 70 80 90 100
RGIHTLEHLF AGFMRNHLNG NGVEIIDISP MGCRTGFYMS LIGTPDEQRV
110 120 130 140 150
ADAWKAAMED VLKVQDQNQI PELNVYQCGT YQMHSLQEAQ DIARSILERD
160 170
VRINSNEELA LPKEKLQELH I
Length:171
Mass (Da):19,416
Last modified:January 23, 2007 - v3
Checksum:i131F57F1866DA105
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311D → E in AAB40286. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC75734.1.
AP009048 Genomic DNA. Translation: BAA16549.1.
U83186 Genomic DNA. Translation: AAB40286.1.
M86657 Genomic DNA. No translation available.
PIRiH65048.
RefSeqiNP_417172.1. NC_000913.3.
YP_490901.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75734; AAC75734; b2687.
BAA16549; BAA16549; BAA16549.
GeneIDi12934186.
947168.
KEGGiecj:Y75_p2630.
eco:b2687.
PATRICi32120768. VBIEscCol129921_2781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC75734.1 .
AP009048 Genomic DNA. Translation: BAA16549.1 .
U83186 Genomic DNA. Translation: AAB40286.1 .
M86657 Genomic DNA. No translation available.
PIRi H65048.
RefSeqi NP_417172.1. NC_000913.3.
YP_490901.1. NC_007779.1.

3D structure databases

ProteinModelPortali P45578.
SMRi P45578. Positions 3-161.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10131N.
IntActi P45578. 5 interactions.
STRINGi 511145.b2687.

Chemistry

BindingDBi P45578.
ChEMBLi CHEMBL3496.

2D gel databases

SWISS-2DPAGE P45578.

Proteomic databases

PaxDbi P45578.
PRIDEi P45578.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75734 ; AAC75734 ; b2687 .
BAA16549 ; BAA16549 ; BAA16549 .
GeneIDi 12934186.
947168.
KEGGi ecj:Y75_p2630.
eco:b2687.
PATRICi 32120768. VBIEscCol129921_2781.

Organism-specific databases

EchoBASEi EB2573.
EcoGenei EG12712. luxS.

Phylogenomic databases

eggNOGi COG1854.
HOGENOMi HOG000040371.
InParanoidi P45578.
KOi K07173.
OMAi RDHLNSD.
OrthoDBi EOG68WRBM.
PhylomeDBi P45578.

Enzyme and pathway databases

BioCyci EcoCyc:EG12712-MONOMER.
ECOL316407:JW2662-MONOMER.
MetaCyc:EG12712-MONOMER.
BRENDAi 4.4.1.21. 2026.
SABIO-RK P45578.

Miscellaneous databases

PROi P45578.

Gene expression databases

Genevestigatori P45578.

Family and domain databases

Gene3Di 3.30.1360.80. 1 hit.
HAMAPi MF_00091. LuxS.
InterProi IPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
[Graphical view ]
Pfami PF02664. LuxS. 1 hit.
[Graphical view ]
PIRSFi PIRSF006160. AI2. 1 hit.
PRINTSi PR01487. LUXSPROTEIN.
ProDomi PD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF63411. SSF63411. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-76.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Emr, an Escherichia coli locus for multidrug resistance."
    Lomovskaya O., Lewis K.
    Proc. Natl. Acad. Sci. U.S.A. 89:8938-8942(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-171.
  6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-10.
    Strain: K12 / EMG2.
  7. "Detection of new genes in a bacterial genome using Markov models for three gene classes."
    Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.
    Nucleic Acids Res. 23:3554-3562(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  8. "Quorum sensing in Escherichia coli and Salmonella typhimurium."
    Surette M.G., Bassler B.L.
    Proc. Natl. Acad. Sci. U.S.A. 95:7046-7050(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production."
    Surette M.G., Miller M.B., Bassler B.L.
    Proc. Natl. Acad. Sci. U.S.A. 96:1639-1644(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiLUXS_ECOLI
AccessioniPrimary (citable) accession number: P45578
Secondary accession number(s): P77134, P77805
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

E.coli strain DH5-alpha does not make AI-2; it has a frameshift mutation in the luxS gene that disrupts the protein coding region.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3