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Protein

RNA chaperone ProQ

Gene

proQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA chaperone with significant RNA binding, RNA strand exchange and RNA duplexing activities. May regulate ProP activity through an RNA-based, post-transcriptional mechanism.UniRule annotation1 Publication

GO - Molecular functioni

  • RNA strand annealing activity Source: EcoCyc
  • RNA strand-exchange activity Source: EcoCyc

GO - Biological processi

  • cellular hyperosmotic salinity response Source: CAFA
  • cellular response to DNA damage stimulus Source: EcoCyc
  • osmosensory signaling pathway Source: CAFA
  • positive regulation of proline import across plasma membrane Source: CAFA
  • posttranscriptional regulation of gene expression Source: EcoCyc

Keywordsi

Molecular functionChaperone, RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12866-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA chaperone ProQUniRule annotation
Gene namesi
Name:proQUniRule annotation
Synonyms:yebJ, yobE, yoeC
Ordered Locus Names:b1831, JW5300
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12866. proQ.

Subcellular locationi

  • Cytoplasm UniRule annotation2 Publications

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutation reduces both the rate and the extent of ProP activation by an osmotic upshift, but does not impair transcription or translation of proP.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002146141 – 232RNA chaperone ProQAdd BLAST232

Proteomic databases

PaxDbiP45577.
PRIDEiP45577.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
evgAP0ACZ43EBI-8767901,EBI-548694

Protein-protein interaction databases

BioGridi4260696. 24 interactors.
DIPiDIP-10572N.
IntActiP45577. 43 interactors.
STRINGi316385.ECDH10B_1970.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi183 – 186Combined sources4
Beta strandi196 – 199Combined sources4
Beta strandi203 – 208Combined sources6
Beta strandi210 – 217Combined sources8
Turni227 – 229Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5NBBNMR-A180-232[»]
DisProtiDP00377.
ProteinModelPortaliP45577.
SMRiP45577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Contains an alpha-helical N-terminal domain (FinO-like) and a beta-sheet C-terminal domain (Hfq-like), connected by an unstructured linker. The FinO-like domain serves as a high-affinity RNA-binding domain, whereas the Hfq-like domain is largely responsible for RNA strand exchange and duplexing.3 Publications

Sequence similaritiesi

Belongs to the ProQ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG41073DY. Bacteria.
COG3109. LUCA.
HOGENOMiHOG000290843.
KOiK03607.

Family and domain databases

Gene3Di1.10.1710.10. 1 hit.
HAMAPiMF_00749. ProQ. 1 hit.
InterProiView protein in InterPro
IPR023529. ProQ.
IPR016103. ProQ/FinO.
IPR035236. ProQ_C.
PfamiView protein in Pfam
PF04352. ProQ. 1 hit.
PF17516. ProQ_C. 1 hit.
SMARTiView protein in SMART
SM00945. ProQ. 1 hit.
SUPFAMiSSF48657. SSF48657. 1 hit.

Sequencei

Sequence statusi: Complete.

P45577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENQPKLNSS KEVIAFLAER FPHCFSAEGE ARPLKIGIFQ DLVDRVAGEM
60 70 80 90 100
NLSKTQLRSA LRLYTSSWRY LYGVKPGATR VDLDGNPCGE LDEQHVEHAR
110 120 130 140 150
KQLEEAKARV QAQRAEQQAK KREAAATAGE KEDAPRRERK PRPTTPRRKE
160 170 180 190 200
GAERKPRAQK PVEKAPKTVK APREEQHTPV SDISALTVGQ ALKVKAGQNA
210 220 230
MDATVLEITK DGVRVQLNSG MSLIVRAEHL VF
Length:232
Mass (Da):25,893
Last modified:July 15, 1999 - v2
Checksum:i042BEAA345A3C739
GO

Sequence cautioni

The sequence BAA20566 differs from that shown. Reason: Frameshift at position 151.Curated
The sequence BAA20566 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L48409 Genomic DNA. Translation: AAD41527.1.
U00096 Genomic DNA. Translation: AAC74901.2.
AP009048 Genomic DNA. Translation: BAA15639.2.
D00674 Genomic DNA. Translation: BAA20566.1. Sequence problems.
PIRiG64944.
RefSeqiWP_000431381.1. NZ_LN832404.1.
YP_026161.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74901; AAC74901; b1831.
BAA15639; BAA15639; BAA15639.
GeneIDi948950.
KEGGiecj:JW5300.
eco:b1831.
PATRICifig|1411691.4.peg.419.

Similar proteinsi

Entry informationi

Entry nameiPROQ_ECOLI
AccessioniPrimary (citable) accession number: P45577
Secondary accession number(s): P56606
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 15, 1999
Last modified: August 30, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families