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P45577

- PROQ_ECOLI

UniProt

P45577 - PROQ_ECOLI

Protein

RNA chaperone ProQ

Gene

proQ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    RNA chaperone with significant RNA binding, RNA strand exchange and RNA duplexing activities. May regulate ProP activity through an RNA-based, post-transcriptional mechanism.1 PublicationUniRule annotation

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA strand annealing activity Source: EcoCyc
    3. RNA strand-exchange activity Source: EcoCyc

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: EcoCyc
    2. positive regulation of transport Source: EcoCyc
    3. posttranscriptional regulation of gene expression Source: EcoCyc

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12866-MONOMER.
    ECOL316407:JW5300-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA chaperone ProQUniRule annotation
    Gene namesi
    Name:proQUniRule annotation
    Synonyms:yebJ, yobE, yoeC
    Ordered Locus Names:b1831, JW5300
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12866. proQ.

    Subcellular locationi

    Cytoplasm 2 PublicationsUniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Mutation reduces both the rate and the extent of ProP activation by an osmotic upshift, but does not impair transcription or translation of proP.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 232232RNA chaperone ProQPRO_0000214614Add
    BLAST

    Proteomic databases

    PaxDbiP45577.
    PRIDEiP45577.

    Expressioni

    Gene expression databases

    GenevestigatoriP45577.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    evgAP0ACZ43EBI-8767901,EBI-548694

    Protein-protein interaction databases

    DIPiDIP-10572N.
    IntActiP45577. 43 interactions.
    STRINGi511145.b1831.

    Structurei

    3D structure databases

    DisProtiDP00377.
    ProteinModelPortaliP45577.
    SMRiP45577. Positions 7-111.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Contains an alpha-helical N-terminal domain (FinO-like) and a beta-sheet C-terminal domain (Hfq-like), connected by an unstructured linker. The FinO-like domain serves as a high-affinity RNA-binding domain, whereas the Hfq-like domain is largely responsible for RNA strand exchange and duplexing.3 Publications

    Sequence similaritiesi

    Belongs to the ProQ family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3109.
    HOGENOMiHOG000290843.
    KOiK03607.
    OMAiGELEQQH.
    OrthoDBiEOG6T1WWT.

    Family and domain databases

    Gene3Di1.10.1710.10. 1 hit.
    HAMAPiMF_00749. ProQ.
    InterProiIPR016103. ProQ/FinO.
    IPR023529. Transptr_ProQ.
    [Graphical view]
    PfamiPF04352. ProQ. 1 hit.
    [Graphical view]
    SMARTiSM00945. ProQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF48657. SSF48657. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P45577-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENQPKLNSS KEVIAFLAER FPHCFSAEGE ARPLKIGIFQ DLVDRVAGEM    50
    NLSKTQLRSA LRLYTSSWRY LYGVKPGATR VDLDGNPCGE LDEQHVEHAR 100
    KQLEEAKARV QAQRAEQQAK KREAAATAGE KEDAPRRERK PRPTTPRRKE 150
    GAERKPRAQK PVEKAPKTVK APREEQHTPV SDISALTVGQ ALKVKAGQNA 200
    MDATVLEITK DGVRVQLNSG MSLIVRAEHL VF 232
    Length:232
    Mass (Da):25,893
    Last modified:July 15, 1999 - v2
    Checksum:i042BEAA345A3C739
    GO

    Sequence cautioni

    The sequence BAA20566.1 differs from that shown. Reason: Frameshift at position 151.
    The sequence BAA20566.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L48409 Genomic DNA. Translation: AAD41527.1.
    U00096 Genomic DNA. Translation: AAC74901.2.
    AP009048 Genomic DNA. Translation: BAA15639.2.
    D00674 Genomic DNA. Translation: BAA20566.1. Sequence problems.
    PIRiG64944.
    RefSeqiYP_026161.1. NC_000913.3.
    YP_490093.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74901; AAC74901; b1831.
    BAA15639; BAA15639; BAA15639.
    GeneIDi12931702.
    948950.
    KEGGiecj:Y75_p1807.
    eco:b1831.
    PATRICi32118983. VBIEscCol129921_1909.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L48409 Genomic DNA. Translation: AAD41527.1 .
    U00096 Genomic DNA. Translation: AAC74901.2 .
    AP009048 Genomic DNA. Translation: BAA15639.2 .
    D00674 Genomic DNA. Translation: BAA20566.1 . Sequence problems.
    PIRi G64944.
    RefSeqi YP_026161.1. NC_000913.3.
    YP_490093.1. NC_007779.1.

    3D structure databases

    DisProti DP00377.
    ProteinModelPortali P45577.
    SMRi P45577. Positions 7-111.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10572N.
    IntActi P45577. 43 interactions.
    STRINGi 511145.b1831.

    Proteomic databases

    PaxDbi P45577.
    PRIDEi P45577.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74901 ; AAC74901 ; b1831 .
    BAA15639 ; BAA15639 ; BAA15639 .
    GeneIDi 12931702.
    948950.
    KEGGi ecj:Y75_p1807.
    eco:b1831.
    PATRICi 32118983. VBIEscCol129921_1909.

    Organism-specific databases

    EchoBASEi EB2707.
    EcoGenei EG12866. proQ.

    Phylogenomic databases

    eggNOGi COG3109.
    HOGENOMi HOG000290843.
    KOi K03607.
    OMAi GELEQQH.
    OrthoDBi EOG6T1WWT.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG12866-MONOMER.
    ECOL316407:JW5300-MONOMER.

    Miscellaneous databases

    PROi P45577.

    Gene expression databases

    Genevestigatori P45577.

    Family and domain databases

    Gene3Di 1.10.1710.10. 1 hit.
    HAMAPi MF_00749. ProQ.
    InterProi IPR016103. ProQ/FinO.
    IPR023529. Transptr_ProQ.
    [Graphical view ]
    Pfami PF04352. ProQ. 1 hit.
    [Graphical view ]
    SMARTi SM00945. ProQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48657. SSF48657. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Protein ProQ influences osmotic activation of compatible solute transporter ProP in Escherichia coli K-12."
      Kunte H.J., Crane R.A., Culham D.E., Richmond D., Wood J.M.
      J. Bacteriol. 181:1537-1543(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3."
      Hara H., Yamamoto Y., Higashitani A., Suzuki H., Nishimura Y.
      J. Bacteriol. 173:4799-4813(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 146-232.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Overexpression, purification, and characterization of ProQ, a posttranslational regulator for osmoregulatory transporter ProP of Escherichia coli."
      Smith M.N., Crane R.A., Keates R.A., Wood J.M.
      Biochemistry 43:12979-12989(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-6, SUBCELLULAR LOCATION, DOMAIN.
    7. "Detection of new genes in a bacterial genome using Markov models for three gene classes."
      Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.
      Nucleic Acids Res. 23:3554-3562(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    8. "Structural and functional analysis of ProQ: an osmoregulatory protein of Escherichia coli."
      Smith M.N., Kwok S.C., Hodges R.S., Wood J.M.
      Biochemistry 46:3084-3095(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN.
    9. Cited for: FUNCTION, RNA-BINDING, DOMAIN.

    Entry informationi

    Entry nameiPROQ_ECOLI
    AccessioniPrimary (citable) accession number: P45577
    Secondary accession number(s): P56606
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3