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Protein

RNA chaperone ProQ

Gene

proQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA chaperone with significant RNA binding, RNA strand exchange and RNA duplexing activities. May regulate ProP activity through an RNA-based, post-transcriptional mechanism.UniRule annotation1 Publication

GO - Molecular functioni

  • RNA strand annealing activity Source: EcoCyc
  • RNA strand-exchange activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • posttranscriptional regulation of gene expression Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12866-MONOMER.
ECOL316407:JW5300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA chaperone ProQUniRule annotation
Gene namesi
Name:proQUniRule annotation
Synonyms:yebJ, yobE, yoeC
Ordered Locus Names:b1831, JW5300
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12866. proQ.

Subcellular locationi

  • Cytoplasm UniRule annotation2 Publications

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutation reduces both the rate and the extent of ProP activation by an osmotic upshift, but does not impair transcription or translation of proP.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232RNA chaperone ProQPRO_0000214614Add
BLAST

Proteomic databases

PaxDbiP45577.
PRIDEiP45577.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
evgAP0ACZ43EBI-8767901,EBI-548694

Protein-protein interaction databases

DIPiDIP-10572N.
IntActiP45577. 43 interactions.
STRINGi511145.b1831.

Structurei

3D structure databases

DisProtiDP00377.
ProteinModelPortaliP45577.
SMRiP45577. Positions 7-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Contains an alpha-helical N-terminal domain (FinO-like) and a beta-sheet C-terminal domain (Hfq-like), connected by an unstructured linker. The FinO-like domain serves as a high-affinity RNA-binding domain, whereas the Hfq-like domain is largely responsible for RNA strand exchange and duplexing.3 Publications

Sequence similaritiesi

Belongs to the ProQ family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3109.
HOGENOMiHOG000290843.
KOiK03607.
OMAiGELEQQH.
OrthoDBiEOG6T1WWT.

Family and domain databases

Gene3Di1.10.1710.10. 1 hit.
HAMAPiMF_00749. ProQ.
InterProiIPR023529. ProQ.
IPR016103. ProQ/FinO.
[Graphical view]
PfamiPF04352. ProQ. 1 hit.
[Graphical view]
SMARTiSM00945. ProQ. 1 hit.
[Graphical view]
SUPFAMiSSF48657. SSF48657. 1 hit.

Sequencei

Sequence statusi: Complete.

P45577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENQPKLNSS KEVIAFLAER FPHCFSAEGE ARPLKIGIFQ DLVDRVAGEM
60 70 80 90 100
NLSKTQLRSA LRLYTSSWRY LYGVKPGATR VDLDGNPCGE LDEQHVEHAR
110 120 130 140 150
KQLEEAKARV QAQRAEQQAK KREAAATAGE KEDAPRRERK PRPTTPRRKE
160 170 180 190 200
GAERKPRAQK PVEKAPKTVK APREEQHTPV SDISALTVGQ ALKVKAGQNA
210 220 230
MDATVLEITK DGVRVQLNSG MSLIVRAEHL VF
Length:232
Mass (Da):25,893
Last modified:July 15, 1999 - v2
Checksum:i042BEAA345A3C739
GO

Sequence cautioni

The sequence BAA20566.1 differs from that shown. Reason: Frameshift at position 151. Curated
The sequence BAA20566.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L48409 Genomic DNA. Translation: AAD41527.1.
U00096 Genomic DNA. Translation: AAC74901.2.
AP009048 Genomic DNA. Translation: BAA15639.2.
D00674 Genomic DNA. Translation: BAA20566.1. Sequence problems.
PIRiG64944.
RefSeqiWP_000431381.1. NZ_CP010445.1.
YP_026161.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74901; AAC74901; b1831.
BAA15639; BAA15639; BAA15639.
GeneIDi948950.
KEGGieco:b1831.
PATRICi32118983. VBIEscCol129921_1909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L48409 Genomic DNA. Translation: AAD41527.1.
U00096 Genomic DNA. Translation: AAC74901.2.
AP009048 Genomic DNA. Translation: BAA15639.2.
D00674 Genomic DNA. Translation: BAA20566.1. Sequence problems.
PIRiG64944.
RefSeqiWP_000431381.1. NZ_CP010445.1.
YP_026161.1. NC_000913.3.

3D structure databases

DisProtiDP00377.
ProteinModelPortaliP45577.
SMRiP45577. Positions 7-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10572N.
IntActiP45577. 43 interactions.
STRINGi511145.b1831.

Proteomic databases

PaxDbiP45577.
PRIDEiP45577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74901; AAC74901; b1831.
BAA15639; BAA15639; BAA15639.
GeneIDi948950.
KEGGieco:b1831.
PATRICi32118983. VBIEscCol129921_1909.

Organism-specific databases

EchoBASEiEB2707.
EcoGeneiEG12866. proQ.

Phylogenomic databases

eggNOGiCOG3109.
HOGENOMiHOG000290843.
KOiK03607.
OMAiGELEQQH.
OrthoDBiEOG6T1WWT.

Enzyme and pathway databases

BioCyciEcoCyc:EG12866-MONOMER.
ECOL316407:JW5300-MONOMER.

Miscellaneous databases

PROiP45577.

Family and domain databases

Gene3Di1.10.1710.10. 1 hit.
HAMAPiMF_00749. ProQ.
InterProiIPR023529. ProQ.
IPR016103. ProQ/FinO.
[Graphical view]
PfamiPF04352. ProQ. 1 hit.
[Graphical view]
SMARTiSM00945. ProQ. 1 hit.
[Graphical view]
SUPFAMiSSF48657. SSF48657. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Protein ProQ influences osmotic activation of compatible solute transporter ProP in Escherichia coli K-12."
    Kunte H.J., Crane R.A., Culham D.E., Richmond D., Wood J.M.
    J. Bacteriol. 181:1537-1543(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3."
    Hara H., Yamamoto Y., Higashitani A., Suzuki H., Nishimura Y.
    J. Bacteriol. 173:4799-4813(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 146-232.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Overexpression, purification, and characterization of ProQ, a posttranslational regulator for osmoregulatory transporter ProP of Escherichia coli."
    Smith M.N., Crane R.A., Keates R.A., Wood J.M.
    Biochemistry 43:12979-12989(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-6, SUBCELLULAR LOCATION, DOMAIN.
  7. "Detection of new genes in a bacterial genome using Markov models for three gene classes."
    Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.
    Nucleic Acids Res. 23:3554-3562(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  8. "Structural and functional analysis of ProQ: an osmoregulatory protein of Escherichia coli."
    Smith M.N., Kwok S.C., Hodges R.S., Wood J.M.
    Biochemistry 46:3084-3095(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN.
  9. Cited for: FUNCTION, RNA-BINDING, DOMAIN.

Entry informationi

Entry nameiPROQ_ECOLI
AccessioniPrimary (citable) accession number: P45577
Secondary accession number(s): P56606
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 15, 1999
Last modified: July 22, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.