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Protein

Lipopolysaccharide core heptose(II)-phosphate phosphatase

Gene

ais

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the dephosphorylation of heptose(II) of the outer membrane lipopolysaccharide core.By similarity

Pathwayi: lipopolysaccharide metabolism

This protein is involved in the pathway lipopolysaccharide metabolism, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide metabolism and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G7165-MONOMER.
ECOL316407:JW2246-MONOMER.
UniPathwayiUPA00451.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core heptose(II)-phosphate phosphatase (EC:3.1.3.-)
Alternative name(s):
Polymyxin resistance protein PmrG
Gene namesi
Name:ais
Synonyms:pmrG
Ordered Locus Names:b2252, JW2246
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13155. ais.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 200175Lipopolysaccharide core heptose(II)-phosphate phosphatasePRO_0000064515Add
BLAST

Proteomic databases

EPDiP45565.
PaxDbiP45565.
PRIDEiP45565.

Expressioni

Inductioni

Induced by BasR (By similarity). Expression is significantly induced in the presence of zinc.By similarity1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260487. 11 interactions.
IntActiP45565. 2 interactions.
STRINGi511145.b2252.

Structurei

3D structure databases

ProteinModelPortaliP45565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108WMR. Bacteria.
ENOG4111KYS. LUCA.
HOGENOMiHOG000122412.
OMAiNFTVIVW.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01868. Ais. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR011310. LipoPS_heptP_Pase.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
PIRSFiPIRSF011416. Ais-TraG-AfrS. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAFCRSSLK SKKYIIILLA LAAIAGLGTH AAWSSNGLPR IDNKTLARLA
60 70 80 90 100
QQHPVVVLFR HAERCDRSTN QCLSDKTGIT VKGTQDAREL GNAFSADIPD
110 120 130 140 150
FDLYSSNTVR TIQSATWFSA GKKLTVDKRL LQCGNEIYSA IKDLQSKAPD
160 170 180 190 200
KNIVIFTHNH CLTYIAKDKR DATFKPDYLD GLVMHVEKGK VYLDGEFVNH
Length:200
Mass (Da):22,257
Last modified:November 1, 1997 - v2
Checksum:iA210382654AC6B35
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti200 – 2001H → Y in CAA58754 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83874 Genomic DNA. Translation: CAA58754.1.
U00096 Genomic DNA. Translation: AAC75312.1.
AP009048 Genomic DNA. Translation: BAA16075.1.
PIRiB64996.
RefSeqiNP_416755.1. NC_000913.3.
WP_000879112.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75312; AAC75312; b2252.
BAA16075; BAA16075; BAA16075.
GeneIDi944945.
KEGGiecj:JW2246.
eco:b2252.
PATRICi32119869. VBIEscCol129921_2344.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83874 Genomic DNA. Translation: CAA58754.1.
U00096 Genomic DNA. Translation: AAC75312.1.
AP009048 Genomic DNA. Translation: BAA16075.1.
PIRiB64996.
RefSeqiNP_416755.1. NC_000913.3.
WP_000879112.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP45565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260487. 11 interactions.
IntActiP45565. 2 interactions.
STRINGi511145.b2252.

Proteomic databases

EPDiP45565.
PaxDbiP45565.
PRIDEiP45565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75312; AAC75312; b2252.
BAA16075; BAA16075; BAA16075.
GeneIDi944945.
KEGGiecj:JW2246.
eco:b2252.
PATRICi32119869. VBIEscCol129921_2344.

Organism-specific databases

EchoBASEiEB2949.
EcoGeneiEG13155. ais.

Phylogenomic databases

eggNOGiENOG4108WMR. Bacteria.
ENOG4111KYS. LUCA.
HOGENOMiHOG000122412.
OMAiNFTVIVW.

Enzyme and pathway databases

UniPathwayiUPA00451.
BioCyciEcoCyc:G7165-MONOMER.
ECOL316407:JW2246-MONOMER.

Miscellaneous databases

PROiP45565.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01868. Ais. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR011310. LipoPS_heptP_Pase.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
PIRSFiPIRSF011416. Ais-TraG-AfrS. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAIS_ECOLI
AccessioniPrimary (citable) accession number: P45565
Secondary accession number(s): P77314
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.