P45565 (AIS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipopolysaccharide core heptose(II)-phosphate phosphatase
EC=3.1.3.-

Alternative name(s):
Polymyxin resistance protein pmrG

Gene names

Name:	ais
Synonyms:	pmrG
Ordered Locus Names:	b2252, JW2246

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the dephosphorylation of heptose(II) of the outer membrane lipopolysaccharide core By similarity. HAMAP MF_01868
Pathway	Bacterial outer membrane biogenesis; lipopolysaccharide metabolism. HAMAP MF_01868
Subcellular location	Periplasm By similarity HAMAP MF_01868.
Induction	Induced by BasR By similarity. Expression is significantly induced in the presence of zinc. Ref.5
Sequence similarities	Belongs to the phosphoglycerate mutase family. Ais subfamily.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 200	175	Lipopolysaccharide core heptose(II)-phosphate phosphatase HAMAP MF_01868		PRO_0000064515
Experimental info
	Sequence conflict	200	1	H → Y in CAA58754. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P45565 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: A210382654AC6B35	FASTA	200	22,257
	10 20 30 40 50 60 MLAFCRSSLK SKKYIIILLA LAAIAGLGTH AAWSSNGLPR IDNKTLARLA QQHPVVVLFR 70 80 90 100 110 120 HAERCDRSTN QCLSDKTGIT VKGTQDAREL GNAFSADIPD FDLYSSNTVR TIQSATWFSA 130 140 150 160 170 180 GKKLTVDKRL LQCGNEIYSA IKDLQSKAPD KNIVIFTHNH CLTYIAKDKR DATFKPDYLD 190 200 GLVMHVEKGK VYLDGEFVNH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, sequencing and characterization of an aluminium-inducible Escherichia coli gene." Guzzo A., Macintyre G., Diorio C., Salmon K., Dubow M.S. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc." Lee L.J., Barrett J.A., Poole R.K. J. Bacteriol. 187:1124-1134(2005) [PubMed: 15659689] [Abstract] Cited for: INDUCTION BY ZINC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83874 Genomic DNA. Translation: CAA58754.1. U00096 Genomic DNA. Translation: AAC75312.1. AP009048 Genomic DNA. Translation: BAA16075.1.
PIR	B64996.
RefSeq	NP_416755.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P45565.
SMR	P45565. Positions 56-119.
ModBase	Search...
Protein-protein interaction databases
IntAct	P45565. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000656; EBESCP00000000656; EBESCG00000000547. EBESCT00000014604; EBESCP00000013895; EBESCG00000013665.
GeneID	944945.
GenomeReviews	Gene locus JW2246 in contig AP009048_GR. Gene locus b2252 in contig U00096_GR.
KEGG	ecj:JW2246. eco:b2252.
PATRIC	32119869. VBIEscCol129921_2344.
Organism-specific databases
EchoBASE	EB2949.
EcoGene	EG13155. ais.
Phylogenomic databases
eggNOG	NOG11567.
GeneTree	EBGT00050000010269.
HOGENOM	HBG467150.
OMA	YLDALVM.
ProtClustDB	PRK15416.
Enzyme and pathway databases
BioCyc	EcoCyc:G7165-MONOMER.
Gene expression databases
Genevestigator	P45565.
Family and domain databases
HAMAP	MF_01868. Ais. [Tree]
InterPro	IPR013078. His_Pase_superF_clade-1. IPR011310. LipoPS_heptP_Pase. [Graphical view]
Pfam	PF00300. His_Phos_1. 1 hit. [Graphical view]
PIRSF	PIRSF011416. Ais-TraG-AfrS. 1 hit.
PROSITE	PS00175. PG_MUTASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AIS_ECOLI

Accession

Primary (citable) accession number: P45565
Secondary accession number(s): P77314

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program