Reviewed, UniProtKB/Swiss-Prot P45565 (AIS_ECOLI)

Names and origin

Protein names

Recommended name:
    Protein ais

Gene names

Name:	ais
Ordered Locus Names:	b2252, JW2246

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Induction	By aluminium.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Protein ais		PRO_0000064515
Regions
	Transmembrane	14 – 33	20	Potential
Experimental info
	Sequence conflict	200	1	H → Y in CAA58754. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P45565-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: A210382654AC6B35	FASTA	200	22,257
	10 20 30 40 50 60 MLAFCRSSLK SKKYIIILLA LAAIAGLGTH AAWSSNGLPR IDNKTLARLA QQHPVVVLFR 70 80 90 100 110 120 HAERCDRSTN QCLSDKTGIT VKGTQDAREL GNAFSADIPD FDLYSSNTVR TIQSATWFSA 130 140 150 160 170 180 GKKLTVDKRL LQCGNEIYSA IKDLQSKAPD KNIVIFTHNH CLTYIAKDKR DATFKPDYLD 190 200 GLVMHVEKGK VYLDGEFVNH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Guzzo A., Macintyre G., Diorio C., Salmon K., Dubow M.S. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83874 Genomic DNA. Translation: CAA58754.1. U00096 Genomic DNA. Translation: AAC75312.1. AP009048 Genomic DNA. Translation: BAA16075.1.
PIR	B64996.
RefSeq	AP_002849.1. NP_416755.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	944945.
GenomeReviews	Gene locus JW2246 in contig AP009048_GR. Gene locus b2252 in contig U00096_GR.
KEGG	ecj:JW2246. eco:b2252.
Organism-specific databases
EchoBASE	EB2949.
EcoGene	EG13155. ais.
CMR	Search...
Phylogenomic databases
HOGENOM	P45565.
OMA	P45565. DKRFLQC.
Enzyme and pathway databases
BioCyc	EcoCyc:G7165-MON.
Family and domain databases
InterPro	IPR011310. Ais-TraG-AfrS. IPR013078. PG_mutase. [Graphical view]
Pfam	PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF011416. Ais-TraG-AfrS. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AIS_ECOLI

Accession

Primary (citable) accession number: P45565
Secondary accession number(s): P77314

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)