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Protein

Xanthosine permease

Gene

xapB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Uptake of xanthosine. Driven by a proton motive force. Can also transport other nucleosides such as inosine, adenosine, cytidine, uridine and thymidine.1 Publication

Enzyme regulationi

Transport is abolished by 2,4-dinitrophenol.1 Publication

Kineticsi

  1. KM=136 µM for xanthosine1 Publication

    GO - Molecular functioni

    • nucleoside transmembrane transporter activity Source: EcoliWiki
    • xanthosine transmembrane transporter activity Source: EcoliWiki

    GO - Biological processi

    • nucleobase-containing small molecule metabolic process Source: EcoliWiki
    • nucleoside transmembrane transport Source: GOC
    • nucleoside transport Source: EcoliWiki
    • transport Source: EcoliWiki
    • xanthosine transport Source: GOC
    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:XAPB-MONOMER.
    ECOL316407:JW2397-MONOMER.
    MetaCyc:XAPB-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.10.2. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xanthosine permease1 Publication
    Alternative name(s):
    Xanthosine transporterCurated
    Gene namesi
    Name:xapB1 Publication
    Ordered Locus Names:b2406, JW2397
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13159. xapB.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 99Cytoplasmic1 Publication
    Transmembranei10 – 3021HelicalSequence analysisAdd
    BLAST
    Topological domaini31 – 4111Periplasmic1 PublicationAdd
    BLAST
    Transmembranei42 – 6221HelicalSequence analysisAdd
    BLAST
    Topological domaini63 – 708Cytoplasmic1 Publication
    Transmembranei71 – 9121HelicalSequence analysisAdd
    BLAST
    Transmembranei92 – 11221HelicalSequence analysisAdd
    BLAST
    Topological domaini113 – 13624Cytoplasmic1 PublicationAdd
    BLAST
    Transmembranei137 – 15721HelicalSequence analysisAdd
    BLAST
    Topological domaini158 – 1592Periplasmic1 Publication
    Transmembranei160 – 18021HelicalSequence analysisAdd
    BLAST
    Topological domaini181 – 20929Cytoplasmic1 PublicationAdd
    BLAST
    Transmembranei210 – 23021HelicalSequence analysisAdd
    BLAST
    Topological domaini231 – 25424Periplasmic1 PublicationAdd
    BLAST
    Transmembranei255 – 27521HelicalSequence analysisAdd
    BLAST
    Topological domaini276 – 2772Cytoplasmic1 Publication
    Transmembranei278 – 29821HelicalSequence analysisAdd
    BLAST
    Topological domaini299 – 3068Periplasmic1 Publication
    Transmembranei307 – 32721HelicalSequence analysisAdd
    BLAST
    Topological domaini328 – 34821Cytoplasmic1 PublicationAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence analysisAdd
    BLAST
    Topological domaini370 – 38112Periplasmic1 PublicationAdd
    BLAST
    Transmembranei382 – 40221HelicalSequence analysisAdd
    BLAST
    Topological domaini403 – 41816Cytoplasmic1 Publication1 PublicationAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: EcoliWiki
    • membrane Source: EcoliWiki
    • plasma membrane Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mutant grows very slowly on xanthosine.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 418418Xanthosine permeasePRO_0000066003Add
    BLAST

    Proteomic databases

    PaxDbiP45562.

    Interactioni

    Protein-protein interaction databases

    BioGridi4262006. 18 interactions.
    STRINGi511145.b2406.

    Structurei

    3D structure databases

    ProteinModelPortaliP45562.
    SMRiP45562. Positions 386-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi310 – 3134Poly-LeuSequence analysis

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105EAA. Bacteria.
    ENOG410ZVFU. LUCA.
    HOGENOMiHOG000026502.
    InParanoidiP45562.
    KOiK11537.
    OMAiWVMLINA.
    OrthoDBiEOG6MPWV9.
    PhylomeDBiP45562.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR004740. Nuc_H_symport.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00889. 2A0110. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45562-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSIAMRLKVM SFLQYFIWGS WLVTLGSYMI NTLHFTGANV GMVYSSKGIA
    60 70 80 90 100
    AIIMPGIMGI IADKWLRAER AYMLCHLVCA GVLFYAASVT DPDMMFWVML
    110 120 130 140 150
    VNAMAFMPTI ALSNSVSYSC LAQAGLDPVT AFPPIRVFGT VGFIVAMWAV
    160 170 180 190 200
    SLLHLELSSL QLYIASGASL LLSAYALTLP KIPVAEKKAT TSLASKLGLD
    210 220 230 240 250
    AFVLFKNPRM AIFFLFAMML GAVLQITNVF GNPFLHDFAR NPEFADSFVV
    260 270 280 290 300
    KYPSILLSVS QMAEVGFILT IPFFLKRFGI KTVMLMSMVA WTLRFGFFAY
    310 320 330 340 350
    GDPSTTGFIL LLLSMIVYGC AFDFFNISGS VFVEQEVDSS IRASAQGLFM
    360 370 380 390 400
    TMVNGVGAWV GSILSGMAVD YFSVDGVKDW QTIWLVFAGY ALFLAVIFFF
    410
    GFKYNHDPEK IKHRAVTH
    Length:418
    Mass (Da):46,140
    Last modified:November 1, 1997 - v2
    Checksum:iC056971AE8A719E8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti63 – 8018DKWLR…HLVCA → VQMRARRTCIHAVSPGVC in CAA52048 (PubMed:7559336).CuratedAdd
    BLAST
    Sequence conflicti293 – 2997LRFGFFA → CALASSP in CAA52048 (PubMed:7559336).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X73828 Genomic DNA. Translation: CAA52048.1.
    U00096 Genomic DNA. Translation: AAC75459.1.
    AP009048 Genomic DNA. Translation: BAA16274.1.
    PIRiE65014.
    RefSeqiNP_416901.1. NC_000913.3.
    WP_000020402.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75459; AAC75459; b2406.
    BAA16274; BAA16274; BAA16274.
    GeneIDi946868.
    KEGGiecj:JW2397.
    eco:b2406.
    PATRICi32120193. VBIEscCol129921_2499.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X73828 Genomic DNA. Translation: CAA52048.1.
    U00096 Genomic DNA. Translation: AAC75459.1.
    AP009048 Genomic DNA. Translation: BAA16274.1.
    PIRiE65014.
    RefSeqiNP_416901.1. NC_000913.3.
    WP_000020402.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP45562.
    SMRiP45562. Positions 386-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262006. 18 interactions.
    STRINGi511145.b2406.

    Protein family/group databases

    TCDBi2.A.1.10.2. the major facilitator superfamily (mfs).

    Proteomic databases

    PaxDbiP45562.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75459; AAC75459; b2406.
    BAA16274; BAA16274; BAA16274.
    GeneIDi946868.
    KEGGiecj:JW2397.
    eco:b2406.
    PATRICi32120193. VBIEscCol129921_2499.

    Organism-specific databases

    EchoBASEiEB2951.
    EcoGeneiEG13159. xapB.

    Phylogenomic databases

    eggNOGiENOG4105EAA. Bacteria.
    ENOG410ZVFU. LUCA.
    HOGENOMiHOG000026502.
    InParanoidiP45562.
    KOiK11537.
    OMAiWVMLINA.
    OrthoDBiEOG6MPWV9.
    PhylomeDBiP45562.

    Enzyme and pathway databases

    BioCyciEcoCyc:XAPB-MONOMER.
    ECOL316407:JW2397-MONOMER.
    MetaCyc:XAPB-MONOMER.

    Miscellaneous databases

    PROiP45562.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR004740. Nuc_H_symport.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00889. 2A0110. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Identification and characterization of genes (xapA, xapB, and xapR) involved in xanthosine catabolism in Escherichia coli."
      Seeger C., Poulsen C., Dandanell G.
      J. Bacteriol. 177:5506-5516(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Specificity and topology of the Escherichia coli xanthosine permease, a representative of the NHS subfamily of the major facilitator superfamily."
      Noerholm M.H., Dandanell G.
      J. Bacteriol. 183:4900-4904(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TOPOLOGY.
    6. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiXAPB_ECOLI
    AccessioniPrimary (citable) accession number: P45562
    Secondary accession number(s): P77729
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1997
    Last modified: January 20, 2016
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.