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Protein

Xanthosine permease

Gene

xapB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Uptake of xanthosine. Driven by a proton motive force. Can also transport other nucleosides such as inosine, adenosine, cytidine, uridine and thymidine.1 Publication

Enzyme regulationi

Transport is abolished by 2,4-dinitrophenol.1 Publication

Kineticsi

  1. KM=136 µM for xanthosine1 Publication

    GO - Molecular functioni

    • nucleoside transmembrane transporter activity Source: EcoliWiki
    • xanthosine transmembrane transporter activity Source: EcoliWiki

    GO - Biological processi

    • nucleobase-containing small molecule metabolic process Source: EcoliWiki
    • nucleoside transport Source: EcoliWiki
    • transport Source: EcoliWiki
    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:XAPB-MONOMER.
    ECOL316407:JW2397-MONOMER.
    MetaCyc:XAPB-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.10.2. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xanthosine permease1 Publication
    Alternative name(s):
    Xanthosine transporterCurated
    Gene namesi
    Name:xapB1 Publication
    Ordered Locus Names:b2406, JW2397
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13159. xapB.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 9Cytoplasmic1 Publication9
    Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
    Topological domaini31 – 41Periplasmic1 PublicationAdd BLAST11
    Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
    Topological domaini63 – 70Cytoplasmic1 Publication8
    Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
    Transmembranei92 – 112HelicalSequence analysisAdd BLAST21
    Topological domaini113 – 136Cytoplasmic1 PublicationAdd BLAST24
    Transmembranei137 – 157HelicalSequence analysisAdd BLAST21
    Topological domaini158 – 159Periplasmic1 Publication2
    Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
    Topological domaini181 – 209Cytoplasmic1 PublicationAdd BLAST29
    Transmembranei210 – 230HelicalSequence analysisAdd BLAST21
    Topological domaini231 – 254Periplasmic1 PublicationAdd BLAST24
    Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
    Topological domaini276 – 277Cytoplasmic1 Publication2
    Transmembranei278 – 298HelicalSequence analysisAdd BLAST21
    Topological domaini299 – 306Periplasmic1 Publication8
    Transmembranei307 – 327HelicalSequence analysisAdd BLAST21
    Topological domaini328 – 348Cytoplasmic1 PublicationAdd BLAST21
    Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
    Topological domaini370 – 381Periplasmic1 PublicationAdd BLAST12
    Transmembranei382 – 402HelicalSequence analysisAdd BLAST21
    Topological domaini403 – 418Cytoplasmic1 Publication1 PublicationAdd BLAST16

    GO - Cellular componenti

    • integral component of membrane Source: EcoliWiki
    • membrane Source: EcoliWiki
    • plasma membrane Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mutant grows very slowly on xanthosine.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000660031 – 418Xanthosine permeaseAdd BLAST418

    Proteomic databases

    PaxDbiP45562.
    PRIDEiP45562.

    Interactioni

    Protein-protein interaction databases

    BioGridi4262006. 18 interactors.
    STRINGi511145.b2406.

    Structurei

    3D structure databases

    ProteinModelPortaliP45562.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi310 – 313Poly-LeuSequence analysis4

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105EAA. Bacteria.
    ENOG410ZVFU. LUCA.
    HOGENOMiHOG000026502.
    InParanoidiP45562.
    KOiK11537.
    OMAiWVMLINA.
    PhylomeDBiP45562.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR004740. Nuc_H_symport.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00889. 2A0110. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45562-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSIAMRLKVM SFLQYFIWGS WLVTLGSYMI NTLHFTGANV GMVYSSKGIA
    60 70 80 90 100
    AIIMPGIMGI IADKWLRAER AYMLCHLVCA GVLFYAASVT DPDMMFWVML
    110 120 130 140 150
    VNAMAFMPTI ALSNSVSYSC LAQAGLDPVT AFPPIRVFGT VGFIVAMWAV
    160 170 180 190 200
    SLLHLELSSL QLYIASGASL LLSAYALTLP KIPVAEKKAT TSLASKLGLD
    210 220 230 240 250
    AFVLFKNPRM AIFFLFAMML GAVLQITNVF GNPFLHDFAR NPEFADSFVV
    260 270 280 290 300
    KYPSILLSVS QMAEVGFILT IPFFLKRFGI KTVMLMSMVA WTLRFGFFAY
    310 320 330 340 350
    GDPSTTGFIL LLLSMIVYGC AFDFFNISGS VFVEQEVDSS IRASAQGLFM
    360 370 380 390 400
    TMVNGVGAWV GSILSGMAVD YFSVDGVKDW QTIWLVFAGY ALFLAVIFFF
    410
    GFKYNHDPEK IKHRAVTH
    Length:418
    Mass (Da):46,140
    Last modified:November 1, 1997 - v2
    Checksum:iC056971AE8A719E8
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti63 – 80DKWLR…HLVCA → VQMRARRTCIHAVSPGVC in CAA52048 (PubMed:7559336).CuratedAdd BLAST18
    Sequence conflicti293 – 299LRFGFFA → CALASSP in CAA52048 (PubMed:7559336).Curated7

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X73828 Genomic DNA. Translation: CAA52048.1.
    U00096 Genomic DNA. Translation: AAC75459.1.
    AP009048 Genomic DNA. Translation: BAA16274.1.
    PIRiE65014.
    RefSeqiNP_416901.1. NC_000913.3.
    WP_000020402.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75459; AAC75459; b2406.
    BAA16274; BAA16274; BAA16274.
    GeneIDi946868.
    KEGGiecj:JW2397.
    eco:b2406.
    PATRICi32120193. VBIEscCol129921_2499.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X73828 Genomic DNA. Translation: CAA52048.1.
    U00096 Genomic DNA. Translation: AAC75459.1.
    AP009048 Genomic DNA. Translation: BAA16274.1.
    PIRiE65014.
    RefSeqiNP_416901.1. NC_000913.3.
    WP_000020402.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP45562.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262006. 18 interactors.
    STRINGi511145.b2406.

    Protein family/group databases

    TCDBi2.A.1.10.2. the major facilitator superfamily (mfs).

    Proteomic databases

    PaxDbiP45562.
    PRIDEiP45562.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75459; AAC75459; b2406.
    BAA16274; BAA16274; BAA16274.
    GeneIDi946868.
    KEGGiecj:JW2397.
    eco:b2406.
    PATRICi32120193. VBIEscCol129921_2499.

    Organism-specific databases

    EchoBASEiEB2951.
    EcoGeneiEG13159. xapB.

    Phylogenomic databases

    eggNOGiENOG4105EAA. Bacteria.
    ENOG410ZVFU. LUCA.
    HOGENOMiHOG000026502.
    InParanoidiP45562.
    KOiK11537.
    OMAiWVMLINA.
    PhylomeDBiP45562.

    Enzyme and pathway databases

    BioCyciEcoCyc:XAPB-MONOMER.
    ECOL316407:JW2397-MONOMER.
    MetaCyc:XAPB-MONOMER.

    Miscellaneous databases

    PROiP45562.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR004740. Nuc_H_symport.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00889. 2A0110. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiXAPB_ECOLI
    AccessioniPrimary (citable) accession number: P45562
    Secondary accession number(s): P77729
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1997
    Last modified: November 2, 2016
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.