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Protein

Amelogenin

Gene

AMEL

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the biomineralization of teeth. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Biomineralization

Names & Taxonomyi

Protein namesi
Recommended name:
Amelogenin
Alternative name(s):
Amelogenin 173A/173B
Leucine-rich amelogenin peptide
Short name:
LRAP
Gene namesi
Name:AMEL
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome X

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Chaini17 – 189173AmelogeninPRO_0000001203Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321Phosphoserine1 Publication

Post-translational modificationi

A number of other isoforms are produced by carboxy-terminal processing.

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP45561.

Miscellaneous databases

PMAP-CutDBP45561.

Expressioni

Gene expression databases

ExpressionAtlasiP45561. differential.
GenevisibleiP45561. SS.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL

Structurei

3D structure databases

DisProtiDP00693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amelogenin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00390000009151.
HOGENOMiHOG000231643.
HOVERGENiHBG016835.
InParanoidiP45561.

Family and domain databases

InterProiIPR004116. Amelogenin.
[Graphical view]
PfamiPF02948. Amelogenin. 1 hit.
[Graphical view]
PRINTSiPR01757. AMELOGENIN.
SMARTiSM00818. Amelogenin. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P45561-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTWILFACL LGAAFSMPLP PHPGHPGYIN FSYEVLTPLK WYQNMIRHPY
60 70 80 90 100
TSYGYEPMGG WLHHQIIPVV SQQTPQSHAL QPHHHIPMVP AQQPGIPQQP
110 120 130 140 150
MMPLPGQHSM TPTQHHQPNL PLPAQQPFQP QPVQPQPHQP LQPQSPMHPI
160 170 180
QPLLPQPPLP PMFSMQSLLP DLPLEAWPAT DKTKREEVD
Length:189
Mass (Da):21,387
Last modified:October 1, 1996 - v2
Checksum:i6392212E0A31D00C
GO
Isoform 2 (identifier: P45561-2) [UniParc]FASTAAdd to basket

Also known as: LRAP

The sequence of this isoform differs from the canonical sequence as follows:
     34-45: Missing.

Show »
Length:177
Mass (Da):19,883
Checksum:i9902EC3D6627E6EC
GO
Isoform 3 (identifier: P45561-3) [UniParc]FASTAAdd to basket

Also known as: 173B

The sequence of this isoform differs from the canonical sequence as follows:
     6-6: L → F
     14-16: AFS → SLA

Show »
Length:189
Mass (Da):21,387
Checksum:iBE633A7C727D1B07
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei6 – 61L → F in isoform 3. CuratedVSP_000233
Alternative sequencei14 – 163AFS → SLA in isoform 3. CuratedVSP_000234
Alternative sequencei34 – 4512Missing in isoform 2. CuratedVSP_000232Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43405 mRNA. Translation: AAB41110.1.
U43406 mRNA. Translation: AAB41111.1.
RefSeqiNP_998965.1. NM_213800.1. [P45561-3]
NP_999071.1. NM_213906.1. [P45561-1]
UniGeneiSsc.179.

Genome annotation databases

EnsembliENSSSCT00000013248; ENSSSCP00000012892; ENSSSCG00000012113. [P45561-1]
GeneIDi396714.
396940.
KEGGissc:396714.
ssc:396940.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43405 mRNA. Translation: AAB41110.1.
U43406 mRNA. Translation: AAB41111.1.
RefSeqiNP_998965.1. NM_213800.1. [P45561-3]
NP_999071.1. NM_213906.1. [P45561-1]
UniGeneiSsc.179.

3D structure databases

DisProtiDP00693.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP45561.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000013248; ENSSSCP00000012892; ENSSSCG00000012113. [P45561-1]
GeneIDi396714.
396940.
KEGGissc:396714.
ssc:396940.

Organism-specific databases

CTDi265.

Phylogenomic databases

GeneTreeiENSGT00390000009151.
HOGENOMiHOG000231643.
HOVERGENiHBG016835.
InParanoidiP45561.

Miscellaneous databases

PMAP-CutDBP45561.

Gene expression databases

ExpressionAtlasiP45561. differential.
GenevisibleiP45561. SS.

Family and domain databases

InterProiIPR004116. Amelogenin.
[Graphical view]
PfamiPF02948. Amelogenin. 1 hit.
[Graphical view]
PRINTSiPR01757. AMELOGENIN.
SMARTiSM00818. Amelogenin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, cDNA sequence, and alternative splicing of porcine amelogenin mRNAs."
    Hu C.C., Bartlett J.D., Zhang C.H., Qian Q., Ryu O.H., Simmer J.P.
    J. Dent. Res. 75:1735-1741(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  2. "Amelogenin post-translational modifications: carboxy-terminal processing and the phosphorylation of bovine and porcine 'TRAP' and 'LRAP' amelogenins."
    Fincham A.G., Moradian-Oldak J.
    Biochem. Biophys. Res. Commun. 197:248-255(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-61 AND 78-181, PHOSPHORYLATION AT SER-32.
    Tissue: Tooth.

Entry informationi

Entry nameiAMEL_PIG
AccessioniPrimary (citable) accession number: P45561
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: January 20, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.