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Protein

Phosphotriesterase homology protein

Gene

php

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Its real enzymatic activity is not yet known. It was tested for general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities with the following substrates: p-nitrophenyl acetate, L-alanine nitroanilide, p-nitrophenyl sulfate, bis(p-nitrophenyl) phosphate, paraoxon, and p-nitrophenyl phosphate. No enzymatic activity was detected with any of these non-specific substrates.

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Zinc 1; via tele nitrogenCombined sources1 Publication1
Metal bindingi14Zinc 1; via tele nitrogenCombined sources1 Publication1
Metal bindingi125Zinc 1Combined sources1 Publication1
Metal bindingi125Zinc 2Combined sources1 Publication1
Metal bindingi158Zinc 2; via pros nitrogenCombined sources1 Publication1
Metal bindingi186Zinc 2; via tele nitrogenCombined sources1 Publication1
Metal bindingi243Zinc 1Combined sources1 Publication1

GO - Molecular functioni

  • hydrolase activity, acting on ester bonds Source: InterPro
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7731-MONOMER.
ECOL316407:JW3342-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphotriesterase homology protein
Gene namesi
Name:php
Synonyms:yhfV
Ordered Locus Names:b3379, JW3342
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12917. php.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002053621 – 292Phosphotriesterase homology proteinAdd BLAST292

Proteomic databases

PaxDbiP45548.
PRIDEiP45548.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4260764. 9 interactors.
DIPiDIP-10504N.
IntActiP45548. 2 interactors.
STRINGi511145.b3379.

Structurei

Secondary structure

1292
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 13Combined sources7
Beta strandi15 – 18Combined sources4
Helixi20 – 23Combined sources4
Helixi26 – 28Combined sources3
Helixi33 – 45Combined sources13
Beta strandi48 – 53Combined sources6
Helixi57 – 59Combined sources3
Helixi63 – 73Combined sources11
Beta strandi76 – 81Combined sources6
Helixi86 – 88Combined sources3
Helixi92 – 95Combined sources4
Helixi98 – 110Combined sources13
Beta strandi120 – 127Combined sources8
Helixi135 – 151Combined sources17
Beta strandi155 – 158Combined sources4
Helixi160 – 162Combined sources3
Helixi165 – 174Combined sources10
Helixi179 – 181Combined sources3
Beta strandi182 – 184Combined sources3
Helixi193 – 201Combined sources9
Beta strandi205 – 208Combined sources4
Turni214 – 216Combined sources3
Helixi219 – 231Combined sources13
Helixi235 – 237Combined sources3
Beta strandi238 – 240Combined sources3
Helixi247 – 249Combined sources3
Helixi251 – 253Combined sources3
Helixi260 – 263Combined sources4
Helixi265 – 271Combined sources7
Helixi276 – 283Combined sources8
Helixi285 – 290Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BF6X-ray1.70A/B2-292[»]
4LEFX-ray1.84A/B/C/D/E/F/G/H1-292[»]
ProteinModelPortaliP45548.
SMRiP45548.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45548.

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphotriesterase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41080QX. Bacteria.
COG1735. LUCA.
HOGENOMiHOG000081700.
InParanoidiP45548.
KOiK07048.
OMAiCDAHDHL.
PhylomeDBiP45548.

Family and domain databases

CDDicd00530. PTE. 1 hit.
InterProiIPR017947. AryldialkylPase_Zn-BS.
IPR032466. Metal_Hydrolase.
IPR001559. Phosphotriesterase.
[Graphical view]
PANTHERiPTHR10819. PTHR10819. 1 hit.
PfamiPF02126. PTE. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
PROSITEiPS01322. PHOSPHOTRIESTERASE_1. 1 hit.
PS51347. PHOSPHOTRIESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFDPTGYTL AHEHLHIDLS GFKNNVDCRL DQYAFICQEM NDLMTRGVRN
60 70 80 90 100
VIEMTNRYMG RNAQFMLDVM RETGINVVAC TGYYQDAFFP EHVATRSVQE
110 120 130 140 150
LAQEMVDEIE QGIDGTELKA GIIAEIGTSE GKITPLEEKV FIAAALAHNQ
160 170 180 190 200
TGRPISTHTS FSTMGLEQLA LLQAHGVDLS RVTVGHCDLK DNLDNILKMI
210 220 230 240 250
DLGAYVQFDT IGKNSYYPDE KRIAMLHALR DRGLLNRVML SMDITRRSHL
260 270 280 290
KANGGYGYDY LLTTFIPQLR QSGFSQADVD VMLRENPSQF FQ
Length:292
Mass (Da):32,915
Last modified:November 1, 1995 - v1
Checksum:i1480C6858E5E8230
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58176.1.
U00096 Genomic DNA. Translation: AAC76404.1.
AP009048 Genomic DNA. Translation: BAE77912.1.
PIRiF65132.
RefSeqiNP_417838.1. NC_000913.3.
WP_000007010.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76404; AAC76404; b3379.
BAE77912; BAE77912; BAE77912.
GeneIDi947891.
KEGGiecj:JW3342.
eco:b3379.
PATRICi32122190. VBIEscCol129921_3472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58176.1.
U00096 Genomic DNA. Translation: AAC76404.1.
AP009048 Genomic DNA. Translation: BAE77912.1.
PIRiF65132.
RefSeqiNP_417838.1. NC_000913.3.
WP_000007010.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BF6X-ray1.70A/B2-292[»]
4LEFX-ray1.84A/B/C/D/E/F/G/H1-292[»]
ProteinModelPortaliP45548.
SMRiP45548.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260764. 9 interactors.
DIPiDIP-10504N.
IntActiP45548. 2 interactors.
STRINGi511145.b3379.

Proteomic databases

PaxDbiP45548.
PRIDEiP45548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76404; AAC76404; b3379.
BAE77912; BAE77912; BAE77912.
GeneIDi947891.
KEGGiecj:JW3342.
eco:b3379.
PATRICi32122190. VBIEscCol129921_3472.

Organism-specific databases

EchoBASEiEB2753.
EcoGeneiEG12917. php.

Phylogenomic databases

eggNOGiENOG41080QX. Bacteria.
COG1735. LUCA.
HOGENOMiHOG000081700.
InParanoidiP45548.
KOiK07048.
OMAiCDAHDHL.
PhylomeDBiP45548.

Enzyme and pathway databases

BioCyciEcoCyc:G7731-MONOMER.
ECOL316407:JW3342-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP45548.
PROiP45548.

Family and domain databases

CDDicd00530. PTE. 1 hit.
InterProiIPR017947. AryldialkylPase_Zn-BS.
IPR032466. Metal_Hydrolase.
IPR001559. Phosphotriesterase.
[Graphical view]
PANTHERiPTHR10819. PTHR10819. 1 hit.
PfamiPF02126. PTE. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
PROSITEiPS01322. PHOSPHOTRIESTERASE_1. 1 hit.
PS51347. PHOSPHOTRIESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHP_ECOLI
AccessioniPrimary (citable) accession number: P45548
Secondary accession number(s): Q2M744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.