Phosphotriesterase homology protein - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P45548 (PHP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphotriesterase homology protein

Gene names

Name:	php
Synonyms:	yhfV
Ordered Locus Names:	b3379, JW3342

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Its real enzymatic activity is not yet known. It was tested for general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities with the following substrates: P-nitrophenyl acetate, L-alanine nitroanilide, P-nitrophenyl sulfate, bis(P-nitrophenyl) phosphate, paraoxon, and P-nitrophenyl phosphate. No enzymatic activity was detected with any of these nonspecific substrates.
Cofactor	Binds 2 zinc ions per subunit.
Subunit structure	Monomer.
Sequence similarities	Belongs to the phosphotriesterase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from direct assay Ref.3. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-1123441,EBI-543750

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 292

292

Phosphotriesterase homology protein

PRO_0000205362

Sites

Metal binding

Zinc 1

Metal binding

Zinc 1

Metal binding

125

Zinc 1

Metal binding

125

Zinc 2

Metal binding

158

Zinc 2

Metal binding

186

Zinc 2

Metal binding

243

Zinc 1

Secondary structure

292

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P45548 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1480C6858E5E8230
Show »

FASTA

292

32,915

        10         20         30         40         50         60 
MSFDPTGYTL AHEHLHIDLS GFKNNVDCRL DQYAFICQEM NDLMTRGVRN VIEMTNRYMG 

        70         80         90        100        110        120 
RNAQFMLDVM RETGINVVAC TGYYQDAFFP EHVATRSVQE LAQEMVDEIE QGIDGTELKA 

       130        140        150        160        170        180 
GIIAEIGTSE GKITPLEEKV FIAAALAHNQ TGRPISTHTS FSTMGLEQLA LLQAHGVDLS 

       190        200        210        220        230        240 
RVTVGHCDLK DNLDNILKMI DLGAYVQFDT IGKNSYYPDE KRIAMLHALR DRGLLNRVML 

       250        260        270        280        290 
SMDITRRSHL KANGGYGYDY LLTTFIPQLR QSGFSQADVD VMLRENPSQF FQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Biochemical characterization and crystallographic structure of an Escherichia coli protein from the phosphotriesterase gene family." Buchbinder J.L., Stephenson R.C., Dresser M.J., Pitera J.W., Scanlan T.S., Fletterick R.J. Biochemistry 37:5096-5106(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS), PARTIAL PROTEIN SEQUENCE.
[4]	Erratum Buchbinder J.L., Stephenson R.C., Dresser M.J., Pitera J.W., Scanlan T.S., Fletterick R.J. Biochemistry 37:10860-10860(1998) [PubMed] [Europe PMC] [Abstract]

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18997 Genomic DNA. Translation: AAA58176.1.
U00096 Genomic DNA. Translation: AAC76404.1.
AP009048 Genomic DNA. Translation: BAE77912.1.

PIR

F65132.

RefSeq

NP_417838.1. NC_000913.2.
YP_492053.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BF6	X-ray	1.70	A/B	2-292	[»]

ProteinModelPortal

P45548.

SMR

P45548. Positions 2-292.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10504N.

IntAct

P45548. 2 interactions.

STRING

511145.b3379.

Proteomic databases

PaxDb

P45548.

PRIDE

P45548.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76404; AAC76404; b3379.
BAE77912; BAE77912; BAE77912.

GeneID

12931770.
947891.

KEGG

ecj:Y75_p3797.
eco:b3379.

PATRIC

32122190. VBIEscCol129921_3472.

Organism-specific databases

EchoBASE

EB2753.

EcoGene

EG12917. php.

Phylogenomic databases

eggNOG

COG1735.

HOGENOM

HOG000081700.

K07048.

OMA

VIGHCDL.

ProtClustDB

PRK09875.

Enzyme and pathway databases

BioCyc

EcoCyc:G7731-MONOMER.
ECOL316407:JW3342-MONOMER.

Gene expression databases

Genevestigator

P45548.

Family and domain databases

InterPro

IPR017947. AryldialkylPase_Zn-BS.
IPR001559. Aryldialkylphosphatase.
IPR027280. Php.
[Graphical view]

PANTHER

PTHR10819. PTHR10819. 1 hit.

Pfam

PF02126. PTE. 1 hit.
[Graphical view]

PIRSF

PIRSF016839. PhP. 1 hit.

PROSITE

PS01322. PHOSPHOTRIESTERASE_1. 1 hit.
PS51347. PHOSPHOTRIESTERASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P45548.

Entry information

Entry name

PHP_ECOLI

Accession

Primary (citable) accession number: P45548
Secondary accession number(s): Q2M744

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents