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Protein

Fructoselysine 6-kinase

Gene

frlD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of fructoselysine to fructoselysine 6-phosphate (PubMed:12147680). Functions in a fructoselysine degradation pathway that allows E.coli to grow on fructoselysine or psicoselysine (PubMed:12147680, PubMed:14641112). To a much lesser extenst, is also able to phosphorylate psicoselysine (PubMed:14641112).2 Publications

Catalytic activityi

ATP + fructoselysine = ADP + fructoselysine 6-phosphate.1 Publication

Kineticsi

  1. KM=18 µM for fructoselysine1 Publication
  2. KM=50 µM for ATP1 Publication
  3. KM=10 mM for psicoselysine1 Publication
  1. Vmax=30 µmol/min/mg enzyme with fructoselysine as substrate1 Publication
  2. Vmax=9 µmol/min/mg enzyme with psicoselysine as substrate1 Publication

Pathwayi: fructoselysine degradation

This protein is involved in step 1 of the subpathway that synthesizes D-glucose 6-phosphate and lysine from fructoselysine.2 Publications
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Fructoselysine 6-kinase (frlD)
  2. Fructoselysine 6-phosphate deglycase (frlB)
This subpathway is part of the pathway fructoselysine degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucose 6-phosphate and lysine from fructoselysine, the pathway fructoselysine degradation and in Carbohydrate metabolism.

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7726-MONOMER.
MetaCyc:G7726-MONOMER.
UniPathwayiUPA00784; UER00769.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructoselysine 6-kinase1 Publication (EC:2.7.1.-1 Publication)
Gene namesi
Name:frlD1 Publication
Synonyms:yhfQ
Ordered Locus Names:b3374, JW3337
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12912. frlD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800661 – 261Fructoselysine 6-kinaseAdd BLAST261

Proteomic databases

PaxDbiP45543.
PRIDEiP45543.

Expressioni

Inductioni

Induced by fructoselysine and psicoselysine. Makes part of the frl operon with FrlA, FrlB, FrlC and FrlR.2 Publications

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4262482. 9 interactors.
DIPiDIP-12326N.
IntActiP45543. 9 interactors.
STRINGi316385.ECDH10B_3549.

Structurei

3D structure databases

ProteinModelPortaliP45543.
SMRiP45543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGiENOG4108KPW. Bacteria.
COG0524. LUCA.
HOGENOMiHOG000221482.
InParanoidiP45543.
KOiK10710.
PhylomeDBiP45543.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiView protein in InterPro
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
PfamiView protein in Pfam
PF00294. PfkB. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiView protein in PROSITE
PS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P45543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLATIGDN CVDIYPQLNK AFSGGNAVNV AVYCTRYGIQ PGCITWVGDD
60 70 80 90 100
DYGTKLKQDL ARMGVDISHV HTKHGVTAQT QVELHDNDRV FGDYTEGVMA
110 120 130 140 150
DFALSEEDYA WLAQYDIVHA AIWGHAEDAF PQLHAAGKLT AFDFSDKWDS
160 170 180 190 200
PLWQTLVPHL DFAFASAPQE DETLRLKMKA IVARGAGTVI VTLGENGSIA
210 220 230 240 250
WDGAQFWRQA PEPVTVIDTM GAGDSFIAGF LCGWSAGMTL PQAIAQGTAC
260
AAKTIQYHGA W
Length:261
Mass (Da):28,332
Last modified:November 1, 1995 - v1
Checksum:i30ACC15F901480C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58171.1.
U00096 Genomic DNA. Translation: AAC76399.1.
AP009048 Genomic DNA. Translation: BAE77917.1.
PIRiA65132.
RefSeqiNP_417833.1. NC_000913.3.
WP_000853353.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76399; AAC76399; b3374.
BAE77917; BAE77917; BAE77917.
GeneIDi947886.
KEGGiecj:JW3337.
eco:b3374.
PATRICifig|511145.12.peg.3467.

Similar proteinsi

Entry informationi

Entry nameiFRLD_ECOLI
AccessioniPrimary (citable) accession number: P45543
Secondary accession number(s): Q2M739
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 25, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families