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Protein

Fructoselysine 3-epimerase

Gene

frlC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible interconversion of fructoselysine with its C-3 epimer, psicoselysine. Allows E.coli to utilize psicoselysine for growth. Does not act on psicose or fructoselysine 6-phosphate.1 Publication

Catalytic activityi

D-fructosyl-L-lysine = D-psicosyl-L-lysine.1 Publication

Cofactori

Ni2+1 Publication, Co2+1 PublicationNote: Can use Ni2+ or Co2+ in vitro, and, to a lesser extent, Fe2+ or Mn2+, but not Ca2+ or Cu2+.1 Publication

Enzyme regulationi

Inhibited by Zn2+.1 Publication

Kineticsi

  1. KM=0.5 mM for D-fructosyl-L-lysine1 Publication
  1. Vmax=2.5 µmol/min/mg enzyme for the epimerization of D-fructosyl-L-lysine1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei148Proton donor/acceptorBy similarity1
Metal bindingi148Divalent metal cationBy similarity1
Metal bindingi181Divalent metal cationBy similarity1
Metal bindingi207Divalent metal cation; via pros nitrogenBy similarity1
Active sitei247Proton donor/acceptorBy similarity1
Metal bindingi247Divalent metal cationBy similarity1

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • racemase and epimerase activity, acting on carbohydrates and derivatives Source: EcoCyc

GO - Biological processi

  • amino sugar catabolic process Source: EcoCyc

Keywordsi

Molecular functionIsomerase
LigandCobalt, Metal-binding, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:G7724-MONOMER.
MetaCyc:G7724-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructoselysine 3-epimerase1 Publication (EC:5.1.3.-1 Publication)
Gene namesi
Name:frlC1 Publication
Synonyms:yhfO, yhfP
Ordered Locus Names:b4474, JW5699
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12910. frlC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000873381 – 276Fructoselysine 3-epimeraseAdd BLAST276

Proteomic databases

PaxDbiP45541.
PRIDEiP45541.

Expressioni

Inductioni

Induced by psicoselysine. Makes part of the frl operon with FrlA, FrlB, FrlD and FrlR.1 Publication

Interactioni

Subunit structurei

Homooctamer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

IntActiP45541. 1 interactor.
STRINGi316385.ECDH10B_3548.

Structurei

3D structure databases

ProteinModelPortaliP45541.
SMRiP45541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FrlC family.Curated

Phylogenomic databases

eggNOGiENOG4107Y0V. Bacteria.
COG1082. LUCA.
HOGENOMiHOG000005027.
InParanoidiP45541.
KOiK10709.
PhylomeDBiP45541.

Family and domain databases

InterProiView protein in InterPro
IPR013022. Xyl_isomerase-like_TIM-brl.
PfamiView protein in Pfam
PF01261. AP_endonuc_2. 1 hit.
SUPFAMiSSF51658. SSF51658. 1 hit.

Sequencei

Sequence statusi: Complete.

P45541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTGMFTCGH QRLPIEHAFR DASELGYDGI EIWGGRPHAF APDLKAGGIK
60 70 80 90 100
QIKALAQTYQ MPIIGYTPET NGYPYNMMLG DEHMRRESLD MIKLAMDMAK
110 120 130 140 150
EMNAGYTLIS AAHAGYLTPP NVIWGRLAEN LSELCEYAEN IGMDLILEPL
160 170 180 190 200
TPYESNVVCN ANDVLHALAL VPSPRLFSMV DICAPYVQAE PVMSYFDKLG
210 220 230 240 250
DKLRHLHIVD SDGASDTHYI PGEGKMPLRE LMRDIIERGY EGYCTVELVT
260 270
MYMNEPRLYA RQALERFRAL LPEDER
Length:276
Mass (Da):31,169
Last modified:October 24, 2003 - v3
Checksum:i6C263FC8C4F945EA
GO

Sequence cautioni

The sequence AAA58169 differs from that shown. Reason: Frameshift at position 112.Curated
The sequence AAA58170 differs from that shown. Reason: Frameshift at position 112.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58169.1. Frameshift.
U18997 Genomic DNA. Translation: AAA58170.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48177.1.
AP009048 Genomic DNA. Translation: BAE77918.1.
PIRiH65131.
RefSeqiWP_000847837.1. NZ_LN832404.1.
YP_026213.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48177; AAT48177; b4474.
BAE77918; BAE77918; BAE77918.
GeneIDi2847758.
KEGGiecj:JW5699.
eco:b4474.
PATRICifig|1411691.4.peg.3357.

Similar proteinsi

Entry informationi

Entry nameiFRLC_ECOLI
AccessioniPrimary (citable) accession number: P45541
Secondary accession number(s): P45542, Q2M738
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 24, 2003
Last modified: August 30, 2017
This is version 101 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families