P45539 (FRLA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative fructoselysine transporter FrlA

Gene names

Name:	frlA
Synonyms:	yhfM
Ordered Locus Names:	b3370, JW3333

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in fructoselysine transport. Ref.3
Pathway	Carbohydrate metabolism; fructoselysine degradation.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fructoselysine metabolic process Inferred from mutant phenotype. Source: EcoCyc
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino acid transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Putative fructoselysine transporter FrlA		PRO_0000054247
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	38 – 58	21	Helical; Potential
	Transmembrane	93 – 113	21	Helical; Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	181 – 201	21	Helical; Potential
	Transmembrane	236 – 256	21	Helical; Potential
	Transmembrane	273 – 293	21	Helical; Potential
	Transmembrane	334 – 354	21	Helical; Potential
	Transmembrane	355 – 375	21	Helical; Potential
	Transmembrane	389 – 410	22	Helical; Potential
	Transmembrane	417 – 435	19	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P45539 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: B98DF5944A5E1FEF	FASTA	445	47,577
	10 20 30 40 50 60 MGSQELQRKL GFWAVLAIAV GTTVGSGIFV SVGEVAKAAG TPWLTVLAFV IGGLIVIPQM 70 80 90 100 110 120 CVYAELSTAY PENGADYVYL KNAGSRPLAF LSGWASFWAN DAPSLSIMAL AIVSNLGFLT 130 140 150 160 170 180 PIDPLLGKFI AAGLIIAFML LHLRSVEGGA AFQTLITIAK IIPFTIVIGL GIFWFKAENF 190 200 210 220 230 240 AAPTTTAIGA TGSFMALLAG ISATSWSYTG MASICYMTGE IKNPGKTMPR ALIGSCLLVL 250 260 270 280 290 300 VLYTLLALVI SGLMPFDKLA NSETPISDAL TWIPALGSTA GIFVAITAMI VILGSLSSCV 310 320 330 340 350 360 MYQPRLEYAM AKDNLFFKCF GHVHPKYNTP DVSIILQGAL GIFFIFVSDL TSLLGYFTLV 370 380 390 400 410 420 MCFKNTLTFG SIIWCRKRDD YKPLWRTPAF GLMTTLAIAS SLILVASTFV WAPIPGLICA 430 440 VIVIATGLPA YAFWAKRSRQ LNALS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Identification of a pathway for the utilization of the Amadori product fructoselysine in Escherichia coli." Wiame E., Delpierre G., Collard F., Van Schaftingen E. J. Biol. Chem. 277:42523-42529(2002) [PubMed: 12147680] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA58167.1. Sequence problems. U00096 Genomic DNA. Translation: AAC76395.2. AP009048 Genomic DNA. Translation: BAE77920.1.
PIR	E65131.
RefSeq	NP_417829.2. NC_000913.2.
3D structure databases
ProteinModelPortal	P45539.
SMR	P45539. Positions 7-444.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12322N.
IntAct	P45539. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004537; EBESCP00000004537; EBESCG00000003702. EBESCT00000014287; EBESCP00000013578; EBESCG00000013349.
GeneID	947878.
GenomeReviews	Gene locus JW3333 in contig AP009048_GR. Gene locus b3370 in contig U00096_GR.
KEGG	ecj:JW3333. eco:b3370.
PATRIC	32122174. VBIEscCol129921_3464.
Organism-specific databases
EchoBASE	EB2745.
EcoGene	EG12908. frlA.
Phylogenomic databases
eggNOG	COG0531.
GeneTree	EBGT00050000009005.
HOGENOM	HBG467352.
OMA	TTHYSSS.
PhylomeDB	P45539.
ProtClustDB	PRK11357.
Enzyme and pathway databases
BioCyc	EcoCyc:YHFM-MONOMER.
Gene expression databases
Genevestigator	P45539.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. IPR002293. AA/rel_permease1. [Graphical view]
KO	K03294.
PANTHER	PTHR11785. AA/rel_permease1. 1 hit.
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
PIRSF	PIRSF006060. AA_transporter. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FRLA_ECOLI

Accession

Primary (citable) accession number: P45539
Secondary accession number(s): P76686, Q2M736

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 15, 1998
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program