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Protein

Sulfurtransferase TusD

Gene

tusD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions. Accepts sulfur from TusA and transfers it in turn to TusE.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781Cysteine persulfide intermediate

GO - Molecular functioni

  • sulfur carrier activity Source: EcoCyc
  • sulfurtransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G7714-MONOMER.
ECOL316407:JW3307-MONOMER.
MetaCyc:G7714-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfurtransferase TusD (EC:2.8.1.-)
Alternative name(s):
tRNA 2-thiouridine synthesizing protein D
Gene namesi
Name:tusD
Synonyms:yheN
Ordered Locus Names:b3345, JW3307
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12898. yheN.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi78 – 781C → S: Loss of activity. 2 Publications
Mutagenesisi122 – 1221C → S: Reduced activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 128128Sulfurtransferase TusDPRO_0000214724Add
BLAST

Proteomic databases

PaxDbiP45532.

Interactioni

Subunit structurei

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.2 Publications

Protein-protein interaction databases

BioGridi4262469. 33 interactions.
DIPiDIP-12310N.
IntActiP45532. 1 interaction.
STRINGi511145.b3345.

Structurei

Secondary structure

1
128
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Beta strandi11 – 155Combined sources
Helixi16 – 2914Combined sources
Beta strandi33 – 397Combined sources
Helixi41 – 477Combined sources
Helixi60 – 7112Combined sources
Beta strandi74 – 785Combined sources
Helixi79 – 846Combined sources
Helixi90 – 967Combined sources
Beta strandi107 – 1104Combined sources
Helixi113 – 1219Combined sources
Beta strandi122 – 1276Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15A/D/G1-128[»]
ProteinModelPortaliP45532.
SMRiP45532. Positions 1-128.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45532.

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrE/TusD family.Curated

Phylogenomic databases

eggNOGiENOG4108ZAT. Bacteria.
COG1553. LUCA.
HOGENOMiHOG000229180.
InParanoidiP45532.
KOiK07235.
OMAiVFFYHDG.
OrthoDBiEOG6BCSTH.

Family and domain databases

Gene3Di3.40.1260.10. 1 hit.
HAMAPiMF_00390. Thiourid_synth_D.
InterProiIPR027396. DsrEFH-like.
IPR003787. Sulphur_relay_DrsE/F-like.
IPR017463. Sulphur_relay_TusD/DsrE.
[Graphical view]
PfamiPF02635. DrsE. 1 hit.
[Graphical view]
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03012. sulf_tusD_dsrE. 1 hit.

Sequencei

Sequence statusi: Complete.

P45532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFAIVVTGP AYGTQQASSA FQFAQALIAD GHELSSVFFY REGVYNANQL
60 70 80 90 100
TSPASDEFDL VRAWQQLNAQ HGVALNICVA AALRRGVVDE TEAGRLGLAS
110 120
SNLQQGFTLS GLGALAEASL TCDRVVQF
Length:128
Mass (Da):13,641
Last modified:November 1, 1995 - v1
Checksum:i87B2754849A0FEFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58142.1.
U00096 Genomic DNA. Translation: AAC76370.1.
AP009048 Genomic DNA. Translation: BAE77946.1.
PIRiD65128.
RefSeqiNP_417804.1. NC_000913.3.
WP_001209680.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76370; AAC76370; b3345.
BAE77946; BAE77946; BAE77946.
GeneIDi947852.
KEGGiecj:JW3307.
eco:b3345.
PATRICi32122122. VBIEscCol129921_3438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58142.1.
U00096 Genomic DNA. Translation: AAC76370.1.
AP009048 Genomic DNA. Translation: BAE77946.1.
PIRiD65128.
RefSeqiNP_417804.1. NC_000913.3.
WP_001209680.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15A/D/G1-128[»]
ProteinModelPortaliP45532.
SMRiP45532. Positions 1-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262469. 33 interactions.
DIPiDIP-12310N.
IntActiP45532. 1 interaction.
STRINGi511145.b3345.

Proteomic databases

PaxDbiP45532.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76370; AAC76370; b3345.
BAE77946; BAE77946; BAE77946.
GeneIDi947852.
KEGGiecj:JW3307.
eco:b3345.
PATRICi32122122. VBIEscCol129921_3438.

Organism-specific databases

EchoBASEiEB2735.
EcoGeneiEG12898. yheN.

Phylogenomic databases

eggNOGiENOG4108ZAT. Bacteria.
COG1553. LUCA.
HOGENOMiHOG000229180.
InParanoidiP45532.
KOiK07235.
OMAiVFFYHDG.
OrthoDBiEOG6BCSTH.

Enzyme and pathway databases

BioCyciEcoCyc:G7714-MONOMER.
ECOL316407:JW3307-MONOMER.
MetaCyc:G7714-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP45532.
PROiP45532.

Family and domain databases

Gene3Di3.40.1260.10. 1 hit.
HAMAPiMF_00390. Thiourid_synth_D.
InterProiIPR027396. DsrEFH-like.
IPR003787. Sulphur_relay_DrsE/F-like.
IPR017463. Sulphur_relay_TusD/DsrE.
[Graphical view]
PfamiPF02635. DrsE. 1 hit.
[Graphical view]
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03012. sulf_tusD_dsrE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions."
    Ikeuchi Y., Shigi N., Kato J., Nishimura A., Suzuki T.
    Mol. Cell 21:97-108(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-78, SUBUNIT.
  4. "Structural basis for sulfur relay to RNA mediated by heterohexameric TusBCD complex."
    Numata T., Fukai S., Ikeuchi Y., Suzuki T., Nureki O.
    Structure 14:357-366(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), MUTAGENESIS OF CYS-78 AND CYS-122, SUBUNIT.

Entry informationi

Entry nameiTUSD_ECOLI
AccessioniPrimary (citable) accession number: P45532
Secondary accession number(s): Q2M710
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.