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Protein

Sulfurtransferase TusD

Gene

tusD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions. Accepts sulfur from TusA and transfers it in turn to TusE.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78Cysteine persulfide intermediate1

GO - Molecular functioni

  • sulfur carrier activity Source: EcoCyc
  • sulfurtransferase activity Source: InterPro

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G7714-MONOMER
MetaCyc:G7714-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfurtransferase TusD (EC:2.8.1.-)
Alternative name(s):
tRNA 2-thiouridine synthesizing protein D
Gene namesi
Name:tusD
Synonyms:yheN
Ordered Locus Names:b3345, JW3307
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12898 yheN

Subcellular locationi

GO - Cellular componenti

  • sulfurtransferase complex Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78C → S: Loss of activity. 2 Publications1
Mutagenesisi122C → S: Reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147241 – 128Sulfurtransferase TusDAdd BLAST128

Proteomic databases

PaxDbiP45532
PRIDEiP45532

Interactioni

Subunit structurei

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.2 Publications

Protein-protein interaction databases

BioGridi4262469, 33 interactors
ComplexPortaliCPX-2144 TusBCDE complex
DIPiDIP-12310N
IntActiP45532, 1 interactor
STRINGi316385.ECDH10B_3520

Structurei

Secondary structure

1128
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Beta strandi11 – 15Combined sources5
Helixi16 – 29Combined sources14
Beta strandi33 – 39Combined sources7
Helixi41 – 47Combined sources7
Helixi60 – 71Combined sources12
Beta strandi74 – 78Combined sources5
Helixi79 – 84Combined sources6
Helixi90 – 96Combined sources7
Beta strandi107 – 110Combined sources4
Helixi113 – 121Combined sources9
Beta strandi122 – 127Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15A/D/G1-128[»]
ProteinModelPortaliP45532
SMRiP45532
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45532

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrE/TusD family.Curated

Phylogenomic databases

eggNOGiENOG4108ZAT Bacteria
COG1553 LUCA
HOGENOMiHOG000229180
InParanoidiP45532
KOiK07235
OMAiEGPYNHQ

Family and domain databases

Gene3Di3.40.1260.10, 1 hit
HAMAPiMF_00390 Thiourid_synth_D, 1 hit
InterProiView protein in InterPro
IPR027396 DsrEFH-like
IPR003787 Sulphur_relay_DrsE/F-like
IPR017463 Sulphur_relay_TusD/DsrE
PfamiView protein in Pfam
PF02635 DrsE, 1 hit
SUPFAMiSSF75169 SSF75169, 1 hit
TIGRFAMsiTIGR03012 sulf_tusD_dsrE, 1 hit

Sequencei

Sequence statusi: Complete.

P45532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFAIVVTGP AYGTQQASSA FQFAQALIAD GHELSSVFFY REGVYNANQL
60 70 80 90 100
TSPASDEFDL VRAWQQLNAQ HGVALNICVA AALRRGVVDE TEAGRLGLAS
110 120
SNLQQGFTLS GLGALAEASL TCDRVVQF
Length:128
Mass (Da):13,641
Last modified:November 1, 1995 - v1
Checksum:i87B2754849A0FEFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58142.1
U00096 Genomic DNA Translation: AAC76370.1
AP009048 Genomic DNA Translation: BAE77946.1
PIRiD65128
RefSeqiNP_417804.1, NC_000913.3
WP_001209680.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76370; AAC76370; b3345
BAE77946; BAE77946; BAE77946
GeneIDi947852
KEGGiecj:JW3307
eco:b3345
PATRICifig|1411691.4.peg.3386

Similar proteinsi

Entry informationi

Entry nameiTUSD_ECOLI
AccessioniPrimary (citable) accession number: P45532
Secondary accession number(s): Q2M710
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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