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Protein

Protein TusC

Gene

tusC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G7713-MONOMER.
ECOL316407:JW3306-MONOMER.
MetaCyc:G7713-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TusC
Alternative name(s):
tRNA 2-thiouridine synthesizing protein C
Gene namesi
Name:tusC
Synonyms:yheM
Ordered Locus Names:b3344, JW3306
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12897. tusC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76C → S: No effect. 1 Publication1
Mutagenesisi79C → S: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002148831 – 119Protein TusCAdd BLAST119

Proteomic databases

PaxDbiP45531.
PRIDEiP45531.

Interactioni

Subunit structurei

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.2 Publications

Protein-protein interaction databases

BioGridi4262468. 28 interactors.
DIPiDIP-12309N.
IntActiP45531. 6 interactors.
MINTiMINT-1238866.
STRINGi511145.b3344.

Structurei

Secondary structure

1119
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Turni12 – 14Combined sources3
Helixi17 – 29Combined sources13
Beta strandi35 – 39Combined sources5
Helixi41 – 47Combined sources7
Helixi53 – 56Combined sources4
Helixi62 – 65Combined sources4
Helixi66 – 70Combined sources5
Beta strandi76 – 79Combined sources4
Helixi80 – 85Combined sources6
Beta strandi99 – 101Combined sources3
Helixi103 – 110Combined sources8
Beta strandi114 – 119Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15B/E/H1-119[»]
ProteinModelPortaliP45531.
SMRiP45531.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45531.

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrF/TusC family.Curated

Phylogenomic databases

eggNOGiENOG4108ZKU. Bacteria.
COG2923. LUCA.
HOGENOMiHOG000284344.
InParanoidiP45531.
KOiK07236.
OMAiMRNYIAT.

Family and domain databases

Gene3Di3.40.1260.10. 1 hit.
HAMAPiMF_00389. Thiourid_synth_C. 1 hit.
InterProiIPR027396. DsrEFH-like.
IPR003787. Sulphur_relay_DrsE/F-like.
IPR017462. Sulphur_relay_TusC/DsrF.
[Graphical view]
PfamiPF02635. DrsE. 1 hit.
[Graphical view]
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03010. sulf_tusC_dsrF. 1 hit.

Sequencei

Sequence statusi: Complete.

P45531-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIAFVFST APHGTAAGRE GLDALLATSA LTDDLAVFFI ADGVFQLLPG
60 70 80 90 100
QKPDAVLARD YIATFKLLGL YDIEQCWVCA ASLRERGLDP QTPFVVEATP
110
LEADALRREL ANYDVILRF
Length:119
Mass (Da):13,045
Last modified:November 1, 1995 - v1
Checksum:i2AF593ADC435CA0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58141.1.
U00096 Genomic DNA. Translation: AAC76369.1.
AP009048 Genomic DNA. Translation: BAE77947.1.
PIRiC65128.
RefSeqiNP_417803.1. NC_000913.3.
WP_000820714.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76369; AAC76369; b3344.
BAE77947; BAE77947; BAE77947.
GeneIDi947853.
KEGGiecj:JW3306.
eco:b3344.
PATRICi32122120. VBIEscCol129921_3437.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58141.1.
U00096 Genomic DNA. Translation: AAC76369.1.
AP009048 Genomic DNA. Translation: BAE77947.1.
PIRiC65128.
RefSeqiNP_417803.1. NC_000913.3.
WP_000820714.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15B/E/H1-119[»]
ProteinModelPortaliP45531.
SMRiP45531.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262468. 28 interactors.
DIPiDIP-12309N.
IntActiP45531. 6 interactors.
MINTiMINT-1238866.
STRINGi511145.b3344.

Proteomic databases

PaxDbiP45531.
PRIDEiP45531.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76369; AAC76369; b3344.
BAE77947; BAE77947; BAE77947.
GeneIDi947853.
KEGGiecj:JW3306.
eco:b3344.
PATRICi32122120. VBIEscCol129921_3437.

Organism-specific databases

EchoBASEiEB2734.
EcoGeneiEG12897. tusC.

Phylogenomic databases

eggNOGiENOG4108ZKU. Bacteria.
COG2923. LUCA.
HOGENOMiHOG000284344.
InParanoidiP45531.
KOiK07236.
OMAiMRNYIAT.

Enzyme and pathway databases

BioCyciEcoCyc:G7713-MONOMER.
ECOL316407:JW3306-MONOMER.
MetaCyc:G7713-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP45531.
PROiP45531.

Family and domain databases

Gene3Di3.40.1260.10. 1 hit.
HAMAPiMF_00389. Thiourid_synth_C. 1 hit.
InterProiIPR027396. DsrEFH-like.
IPR003787. Sulphur_relay_DrsE/F-like.
IPR017462. Sulphur_relay_TusC/DsrF.
[Graphical view]
PfamiPF02635. DrsE. 1 hit.
[Graphical view]
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03010. sulf_tusC_dsrF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTUSC_ECOLI
AccessioniPrimary (citable) accession number: P45531
Secondary accession number(s): Q2M709
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.