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Protein

Protein TusC

Gene

tusC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions.1 Publication

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: EcoCyc

Keywordsi

Biological processtRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G7713-MONOMER
MetaCyc:G7713-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TusC
Alternative name(s):
tRNA 2-thiouridine synthesizing protein C
Gene namesi
Name:tusC
Synonyms:yheM
Ordered Locus Names:b3344, JW3306
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12897 tusC

Subcellular locationi

GO - Cellular componenti

  • sulfurtransferase complex Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76C → S: No effect. 1 Publication1
Mutagenesisi79C → S: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002148831 – 119Protein TusCAdd BLAST119

Proteomic databases

PaxDbiP45531
PRIDEiP45531

Interactioni

Subunit structurei

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.2 Publications

Protein-protein interaction databases

BioGridi4262468, 28 interactors
DIPiDIP-12309N
IntActiP45531, 7 interactors
STRINGi316385.ECDH10B_3519

Structurei

Secondary structure

1119
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Turni12 – 14Combined sources3
Helixi17 – 29Combined sources13
Beta strandi35 – 39Combined sources5
Helixi41 – 47Combined sources7
Helixi53 – 56Combined sources4
Helixi62 – 65Combined sources4
Helixi66 – 70Combined sources5
Beta strandi76 – 79Combined sources4
Helixi80 – 85Combined sources6
Beta strandi99 – 101Combined sources3
Helixi103 – 110Combined sources8
Beta strandi114 – 119Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15B/E/H1-119[»]
ProteinModelPortaliP45531
SMRiP45531
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45531

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrF/TusC family.Curated

Phylogenomic databases

eggNOGiENOG4108ZKU Bacteria
COG2923 LUCA
HOGENOMiHOG000284344
InParanoidiP45531
KOiK07236
OMAiLEMILIM

Family and domain databases

Gene3Di3.40.1260.10, 1 hit
HAMAPiMF_00389 Thiourid_synth_C, 1 hit
InterProiView protein in InterPro
IPR027396 DsrEFH-like
IPR003787 Sulphur_relay_DrsE/F-like
IPR037450 Sulphur_relay_TusC
IPR017462 Sulphur_relay_TusC/DsrF
PANTHERiPTHR38780 PTHR38780, 1 hit
PfamiView protein in Pfam
PF02635 DrsE, 1 hit
SUPFAMiSSF75169 SSF75169, 1 hit
TIGRFAMsiTIGR03010 sulf_tusC_dsrF, 1 hit

Sequencei

Sequence statusi: Complete.

P45531-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIAFVFST APHGTAAGRE GLDALLATSA LTDDLAVFFI ADGVFQLLPG
60 70 80 90 100
QKPDAVLARD YIATFKLLGL YDIEQCWVCA ASLRERGLDP QTPFVVEATP
110
LEADALRREL ANYDVILRF
Length:119
Mass (Da):13,045
Last modified:November 1, 1995 - v1
Checksum:i2AF593ADC435CA0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58141.1
U00096 Genomic DNA Translation: AAC76369.1
AP009048 Genomic DNA Translation: BAE77947.1
PIRiC65128
RefSeqiNP_417803.1, NC_000913.3
WP_000820714.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76369; AAC76369; b3344
BAE77947; BAE77947; BAE77947
GeneIDi947853
KEGGiecj:JW3306
eco:b3344
PATRICifig|1411691.4.peg.3387

Similar proteinsi

Entry informationi

Entry nameiTUSC_ECOLI
AccessioniPrimary (citable) accession number: P45531
Secondary accession number(s): Q2M709
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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