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Protein

Protein TusC

Gene

tusC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions.1 Publication

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: EcoCyc

Keywordsi

Biological processtRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G7713-MONOMER.
MetaCyc:G7713-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TusC
Alternative name(s):
tRNA 2-thiouridine synthesizing protein C
Gene namesi
Name:tusC
Synonyms:yheM
Ordered Locus Names:b3344, JW3306
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12897. tusC.

Subcellular locationi

GO - Cellular componenti

  • sulfurtransferase complex Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76C → S: No effect. 1 Publication1
Mutagenesisi79C → S: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002148831 – 119Protein TusCAdd BLAST119

Proteomic databases

PaxDbiP45531.
PRIDEiP45531.

Interactioni

Subunit structurei

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.2 Publications

Protein-protein interaction databases

BioGridi4262468. 28 interactors.
DIPiDIP-12309N.
IntActiP45531. 6 interactors.
MINTiMINT-1238866.
STRINGi316385.ECDH10B_3519.

Structurei

Secondary structure

1119
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Turni12 – 14Combined sources3
Helixi17 – 29Combined sources13
Beta strandi35 – 39Combined sources5
Helixi41 – 47Combined sources7
Helixi53 – 56Combined sources4
Helixi62 – 65Combined sources4
Helixi66 – 70Combined sources5
Beta strandi76 – 79Combined sources4
Helixi80 – 85Combined sources6
Beta strandi99 – 101Combined sources3
Helixi103 – 110Combined sources8
Beta strandi114 – 119Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15B/E/H1-119[»]
ProteinModelPortaliP45531.
SMRiP45531.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45531.

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrF/TusC family.Curated

Phylogenomic databases

eggNOGiENOG4108ZKU. Bacteria.
COG2923. LUCA.
HOGENOMiHOG000284344.
InParanoidiP45531.
KOiK07236.

Family and domain databases

HAMAPiMF_00389. Thiourid_synth_C. 1 hit.
InterProiView protein in InterPro
IPR027396. DsrEFH-like.
IPR003787. Sulphur_relay_DrsE/F-like.
IPR017462. Sulphur_relay_TusC/DsrF.
PfamiView protein in Pfam
PF02635. DrsE. 1 hit.
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03010. sulf_tusC_dsrF. 1 hit.

Sequencei

Sequence statusi: Complete.

P45531-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIAFVFST APHGTAAGRE GLDALLATSA LTDDLAVFFI ADGVFQLLPG
60 70 80 90 100
QKPDAVLARD YIATFKLLGL YDIEQCWVCA ASLRERGLDP QTPFVVEATP
110
LEADALRREL ANYDVILRF
Length:119
Mass (Da):13,045
Last modified:November 1, 1995 - v1
Checksum:i2AF593ADC435CA0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58141.1.
U00096 Genomic DNA. Translation: AAC76369.1.
AP009048 Genomic DNA. Translation: BAE77947.1.
PIRiC65128.
RefSeqiNP_417803.1. NC_000913.3.
WP_000820714.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76369; AAC76369; b3344.
BAE77947; BAE77947; BAE77947.
GeneIDi947853.
KEGGiecj:JW3306.
eco:b3344.
PATRICifig|1411691.4.peg.3387.

Similar proteinsi

Entry informationi

Entry nameiTUSC_ECOLI
AccessioniPrimary (citable) accession number: P45531
Secondary accession number(s): Q2M709
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: August 30, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries