P45530 (TUSB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein TusB

Alternative name(s):
tRNA 2-thiouridine synthesizing protein B

Gene names

Name:	tusB
Synonyms:	yheL
Ordered Locus Names:	b3343, JW3305

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions. Ref.3
Subunit structure	Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE. Ref.3 Ref.4
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_01564.
Sequence similarities	Belongs to the DsrH/TusB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA wobble position uridine thiolation Inferred from mutant phenotype Ref.3. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 95

95

Protein TusB HAMAP-Rule MF_01564

PRO_0000169508

Secondary structure

1

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95

Helix Strand Turn

Details...

Beta strand

2 – 5

4

Helix

9 – 11

3

Helix

14 – 19

6

Beta strand

26 – 29

4

Helix

31 – 37

7

Helix

44 – 48

5

Beta strand

50 – 57

8

Helix

58 – 63

6

Beta strand

74 – 77

4

Helix

79 – 88

10

Beta strand

92 – 94

3

Sequences

	Sequence		Length	Mass (Da)
	P45530 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 0EEF7560CCB4D608	FASTA	95	10,718
	10 20 30 40 50 60 MLHTLHRSPW LTDFAALLRL LSEGDELLLL QDGVTAAVDG NRYLESLRNA PIKVYALNED 70 80 90 LIARGLTGQI SNDIILIDYT DFVRLTVKHP SQMAW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions." Ikeuchi Y., Shigi N., Kato J., Nishimura A., Suzuki T. Mol. Cell 21:97-108(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[4]	"Structural basis for sulfur relay to RNA mediated by heterohexameric TusBCD complex." Numata T., Fukai S., Ikeuchi Y., Suzuki T., Nureki O. Structure 14:357-366(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18997 Genomic DNA. Translation: AAA58140.1.
U00096 Genomic DNA. Translation: AAC76368.1.
AP009048 Genomic DNA. Translation: BAE77948.1.

PIR

B65128.

RefSeq

NP_417802.1. NC_000913.2.
YP_492089.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D1P	X-ray	2.15	C/F/I	1-95	[»]

ProteinModelPortal

P45530.

SMR

P45530. Positions 1-95.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-12308N.

STRING

511145.b3343.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76368; AAC76368; b3343.
BAE77948; BAE77948; BAE77948.

GeneID

12932623.
947844.

KEGG

ecj:Y75_p3833.
eco:b3343.

PATRIC

32122118. VBIEscCol129921_3436.

Organism-specific databases

EchoBASE

EB2733.

EcoGene

EG12896. tusB.

Phylogenomic databases

eggNOG

COG2168.

HOGENOM

HOG000284333.

KO

K07237.

OMA

EFVQLTV.

ProtClustDB

PRK13510.

Enzyme and pathway databases

BioCyc

EcoCyc:G7712-MONOMER.
ECOL316407:JW3305-MONOMER.
MetaCyc:G7712-MONOMER.

Gene expression databases

Genevestigator

P45530.

Family and domain databases

HAMAP

MF_01564. Thiourid_synth_B.

InterPro

IPR023526. Sulphur_relay_TusB.
IPR007215. Sulphur_relay_TusB/DsrH.
[Graphical view]

Pfam

PF04077. DsrH. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03011. sulf_tusB_dsrH. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P45530.

Entry information

Entry name

TUSB_ECOLI

Accession

Primary (citable) accession number: P45530
Secondary accession number(s): Q2M708

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program