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Protein

Protein TusB

Gene

tusB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions.1 Publication

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G7712-MONOMER.
ECOL316407:JW3305-MONOMER.
MetaCyc:G7712-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TusB
Alternative name(s):
tRNA 2-thiouridine synthesizing protein B
Gene namesi
Name:tusB
Synonyms:yheL
Ordered Locus Names:b3343, JW3305
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12896. tusB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9595Protein TusBPRO_0000169508Add
BLAST

Proteomic databases

EPDiP45530.
PaxDbiP45530.

Interactioni

Subunit structurei

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.2 Publications

Protein-protein interaction databases

BioGridi4261617. 51 interactions.
DIPiDIP-12308N.
IntActiP45530. 3 interactions.
STRINGi511145.b3343.

Structurei

Secondary structure

1
95
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54Combined sources
Helixi9 – 113Combined sources
Helixi14 – 196Combined sources
Beta strandi26 – 294Combined sources
Helixi31 – 377Combined sources
Helixi44 – 485Combined sources
Beta strandi50 – 578Combined sources
Helixi58 – 636Combined sources
Beta strandi74 – 774Combined sources
Helixi79 – 8810Combined sources
Beta strandi92 – 943Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15C/F/I1-95[»]
ProteinModelPortaliP45530.
SMRiP45530. Positions 1-95.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45530.

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrH/TusB family.Curated

Phylogenomic databases

eggNOGiENOG4105YKK. Bacteria.
COG2168. LUCA.
HOGENOMiHOG000284333.
InParanoidiP45530.
KOiK07237.
OMAiHASQMAW.
OrthoDBiEOG6MWNF1.

Family and domain databases

Gene3Di3.40.1260.10. 1 hit.
HAMAPiMF_01564. Thiourid_synth_B.
InterProiIPR027396. DsrEFH-like.
IPR023526. Sulphur_relay_TusB.
IPR007215. Sulphur_relay_TusB/DsrH.
[Graphical view]
PfamiPF04077. DsrH. 1 hit.
[Graphical view]
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03011. sulf_tusB_dsrH. 1 hit.

Sequencei

Sequence statusi: Complete.

P45530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHTLHRSPW LTDFAALLRL LSEGDELLLL QDGVTAAVDG NRYLESLRNA
60 70 80 90
PIKVYALNED LIARGLTGQI SNDIILIDYT DFVRLTVKHP SQMAW
Length:95
Mass (Da):10,718
Last modified:November 1, 1995 - v1
Checksum:i0EEF7560CCB4D608
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58140.1.
U00096 Genomic DNA. Translation: AAC76368.1.
AP009048 Genomic DNA. Translation: BAE77948.1.
PIRiB65128.
RefSeqiNP_417802.1. NC_000913.3.
WP_000903373.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76368; AAC76368; b3343.
BAE77948; BAE77948; BAE77948.
GeneIDi947844.
KEGGiecj:JW3305.
eco:b3343.
PATRICi32122118. VBIEscCol129921_3436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58140.1.
U00096 Genomic DNA. Translation: AAC76368.1.
AP009048 Genomic DNA. Translation: BAE77948.1.
PIRiB65128.
RefSeqiNP_417802.1. NC_000913.3.
WP_000903373.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15C/F/I1-95[»]
ProteinModelPortaliP45530.
SMRiP45530. Positions 1-95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261617. 51 interactions.
DIPiDIP-12308N.
IntActiP45530. 3 interactions.
STRINGi511145.b3343.

Proteomic databases

EPDiP45530.
PaxDbiP45530.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76368; AAC76368; b3343.
BAE77948; BAE77948; BAE77948.
GeneIDi947844.
KEGGiecj:JW3305.
eco:b3343.
PATRICi32122118. VBIEscCol129921_3436.

Organism-specific databases

EchoBASEiEB2733.
EcoGeneiEG12896. tusB.

Phylogenomic databases

eggNOGiENOG4105YKK. Bacteria.
COG2168. LUCA.
HOGENOMiHOG000284333.
InParanoidiP45530.
KOiK07237.
OMAiHASQMAW.
OrthoDBiEOG6MWNF1.

Enzyme and pathway databases

BioCyciEcoCyc:G7712-MONOMER.
ECOL316407:JW3305-MONOMER.
MetaCyc:G7712-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP45530.
PROiP45530.

Family and domain databases

Gene3Di3.40.1260.10. 1 hit.
HAMAPiMF_01564. Thiourid_synth_B.
InterProiIPR027396. DsrEFH-like.
IPR023526. Sulphur_relay_TusB.
IPR007215. Sulphur_relay_TusB/DsrH.
[Graphical view]
PfamiPF04077. DsrH. 1 hit.
[Graphical view]
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03011. sulf_tusB_dsrH. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions."
    Ikeuchi Y., Shigi N., Kato J., Nishimura A., Suzuki T.
    Mol. Cell 21:97-108(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  4. "Structural basis for sulfur relay to RNA mediated by heterohexameric TusBCD complex."
    Numata T., Fukai S., Ikeuchi Y., Suzuki T., Nureki O.
    Structure 14:357-366(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiTUSB_ECOLI
AccessioniPrimary (citable) accession number: P45530
Secondary accession number(s): Q2M708
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 16, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.