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Protein

Protein TusB

Gene

tusB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions.1 Publication

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: EcoCyc

Keywordsi

Biological processtRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G7712-MONOMER.
MetaCyc:G7712-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TusB
Alternative name(s):
tRNA 2-thiouridine synthesizing protein B
Gene namesi
Name:tusB
Synonyms:yheL
Ordered Locus Names:b3343, JW3305
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12896. tusB.

Subcellular locationi

GO - Cellular componenti

  • sulfurtransferase complex Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001695081 – 95Protein TusBAdd BLAST95

Proteomic databases

PaxDbiP45530.
PRIDEiP45530.

Interactioni

Subunit structurei

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.2 Publications

Protein-protein interaction databases

BioGridi4261617. 51 interactors.
DIPiDIP-12308N.
IntActiP45530. 3 interactors.
STRINGi316385.ECDH10B_3518.

Structurei

Secondary structure

195
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi9 – 11Combined sources3
Helixi14 – 19Combined sources6
Beta strandi26 – 29Combined sources4
Helixi31 – 37Combined sources7
Helixi44 – 48Combined sources5
Beta strandi50 – 57Combined sources8
Helixi58 – 63Combined sources6
Beta strandi74 – 77Combined sources4
Helixi79 – 88Combined sources10
Beta strandi92 – 94Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1PX-ray2.15C/F/I1-95[»]
ProteinModelPortaliP45530.
SMRiP45530.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45530.

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrH/TusB family.Curated

Phylogenomic databases

eggNOGiENOG4105YKK. Bacteria.
COG2168. LUCA.
HOGENOMiHOG000284333.
InParanoidiP45530.
KOiK07237.

Family and domain databases

HAMAPiMF_01564. Thiourid_synth_B. 1 hit.
InterProiView protein in InterPro
IPR027396. DsrEFH-like.
IPR023526. Sulphur_relay_TusB.
IPR007215. Sulphur_relay_TusB/DsrH.
PfamiView protein in Pfam
PF04077. DsrH. 1 hit.
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03011. sulf_tusB_dsrH. 1 hit.

Sequencei

Sequence statusi: Complete.

P45530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHTLHRSPW LTDFAALLRL LSEGDELLLL QDGVTAAVDG NRYLESLRNA
60 70 80 90
PIKVYALNED LIARGLTGQI SNDIILIDYT DFVRLTVKHP SQMAW
Length:95
Mass (Da):10,718
Last modified:November 1, 1995 - v1
Checksum:i0EEF7560CCB4D608
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58140.1.
U00096 Genomic DNA. Translation: AAC76368.1.
AP009048 Genomic DNA. Translation: BAE77948.1.
PIRiB65128.
RefSeqiNP_417802.1. NC_000913.3.
WP_000903373.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76368; AAC76368; b3343.
BAE77948; BAE77948; BAE77948.
GeneIDi947844.
KEGGiecj:JW3305.
eco:b3343.
PATRICifig|1411691.4.peg.3388.

Similar proteinsi

Entry informationi

Entry nameiTUSB_ECOLI
AccessioniPrimary (citable) accession number: P45530
Secondary accession number(s): Q2M708
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: August 30, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families