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Protein

Glutathione-regulated potassium-efflux system protein KefB

Gene

kefB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pore-forming subunit of a potassium efflux system that confers protection against electrophiles. Catalyzes K+/H+ antiport.UniRule annotation2 Publications

Enzyme regulationi

Activated by adducts between glutathione and electrophiles.1 Publication

GO - Molecular functioni

GO - Biological processi

  • potassium ion transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciEcoCyc:KEFB-MONOMER.
ECOL316407:JW3313-MONOMER.
MetaCyc:KEFB-MONOMER.

Protein family/group databases

TCDBi2.A.37.1.2. the monovalent cation:proton antiporter-2 (cpa2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-regulated potassium-efflux system protein KefBUniRule annotation
Alternative name(s):
K(+)/H(+) antiporterUniRule annotation
NEM-activable K(+)/H(+) antiporter
Gene namesi
Name:kefBUniRule annotation
Synonyms:trkB
Ordered Locus Names:b3350, JW3313
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20110. kefB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24HelicalUniRule annotationAdd BLAST21
Transmembranei29 – 49HelicalUniRule annotationAdd BLAST21
Transmembranei55 – 75HelicalUniRule annotationAdd BLAST21
Transmembranei87 – 107HelicalUniRule annotationAdd BLAST21
Transmembranei115 – 135HelicalUniRule annotationAdd BLAST21
Transmembranei152 – 172HelicalUniRule annotationAdd BLAST21
Transmembranei177 – 197HelicalUniRule annotationAdd BLAST21
Transmembranei207 – 227HelicalUniRule annotationAdd BLAST21
Transmembranei230 – 250HelicalUniRule annotationAdd BLAST21
Transmembranei268 – 288HelicalUniRule annotationAdd BLAST21
Transmembranei291 – 311HelicalUniRule annotationAdd BLAST21
Transmembranei324 – 344HelicalUniRule annotationAdd BLAST21
Transmembranei356 – 376HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001965961 – 601Glutathione-regulated potassium-efflux system protein KefBAdd BLAST601

Proteomic databases

PaxDbiP45522.
PRIDEiP45522.

Interactioni

Subunit structurei

Interacts with the regulatory subunit KefG.UniRule annotation

Protein-protein interaction databases

BioGridi4262061. 11 interactors.
STRINGi511145.b3350.

Structurei

3D structure databases

ProteinModelPortaliP45522.
SMRiP45522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini402 – 524RCK N-terminalUniRule annotationAdd BLAST123

Sequence similaritiesi

Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. KefB subfamily. [View classification]UniRule annotation
Contains 1 RCK N-terminal domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CKD. Bacteria.
COG0475. LUCA.
COG1226. LUCA.
HOGENOMiHOG000179076.
InParanoidiP45522.
KOiK11747.
OMAiRELMPVH.
PhylomeDBiP45522.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01412. K_H_efflux_KefB. 1 hit.
InterProiIPR006153. Cation/H_exchanger.
IPR004771. K/H_exchanger.
IPR020884. K_H_efflux_KefB.
IPR016040. NAD(P)-bd_dom.
IPR003148. RCK_N.
[Graphical view]
PfamiPF00999. Na_H_Exchanger. 1 hit.
PF02254. TrkA_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00932. 2a37. 1 hit.
PROSITEiPS51201. RCK_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSDFLLAG VLFLFAAVAA VPLASRLGIG AVLGYLLAGI AIGPWGLGFI
60 70 80 90 100
SDVDEILHFS ELGVVFLMFI IGLELNPSKL WQLRRSIFGV GAAQVLLSAA
110 120 130 140 150
LLAGLLMLTD FAWQAAVVGG IGLAMSSTAM ALQLMREKGM NRSESGQLGF
160 170 180 190 200
SVLLFQDLAV IPALALVPLL AGSADEHFDW MKVGMKVLAF VGMLIGGRYL
210 220 230 240 250
LRPVFRFIAA SGVREVFTAA TLLLVLGSAL FMDALGLSMA LGTFIAGVLL
260 270 280 290 300
AESEYRHELE TAIDPFKGLL LGLFFISVGM SLNLGVLYTH LLWVVISVVV
310 320 330 340 350
LVAVKILVLY LLARLYGVRS SERMQFAGVL SQGGEFAFVL FSTASSQRLF
360 370 380 390 400
QGDQMALLLV TVTLSMMTTP LLMKLVDKWL SRQFNGPEEE DEKPWVNDDK
410 420 430 440 450
PQVIVVGFGR FGQVIGRLLM ANKMRITVLE RDISAVNLMR KYGYKVYYGD
460 470 480 490 500
ATQVDLLRSA GAEAAESIVI TCNEPEDTMK LVEICQQHFP HLHILARARG
510 520 530 540 550
RVEAHELLQA GVTQFSRETF SSALELGRKT LVTLGMHPHQ AQRAQLHFRR
560 570 580 590 600
LDMRMLRELI PMHADTVQIS RAREARRELE EIFQREMQQE RRQLDGWDEF

E
Length:601
Mass (Da):66,411
Last modified:November 1, 1995 - v1
Checksum:iB7534C7F4541497E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58147.1.
U00096 Genomic DNA. Translation: AAC76375.1.
AP009048 Genomic DNA. Translation: BAE77940.1.
PIRiA65129.
RefSeqiNP_417809.1. NC_000913.3.
WP_000399147.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76375; AAC76375; b3350.
BAE77940; BAE77940; BAE77940.
GeneIDi947858.
KEGGiecj:JW3313.
eco:b3350.
PATRICi32122134. VBIEscCol129921_3444.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58147.1.
U00096 Genomic DNA. Translation: AAC76375.1.
AP009048 Genomic DNA. Translation: BAE77940.1.
PIRiA65129.
RefSeqiNP_417809.1. NC_000913.3.
WP_000399147.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP45522.
SMRiP45522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262061. 11 interactors.
STRINGi511145.b3350.

Protein family/group databases

TCDBi2.A.37.1.2. the monovalent cation:proton antiporter-2 (cpa2) family.

Proteomic databases

PaxDbiP45522.
PRIDEiP45522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76375; AAC76375; b3350.
BAE77940; BAE77940; BAE77940.
GeneIDi947858.
KEGGiecj:JW3313.
eco:b3350.
PATRICi32122134. VBIEscCol129921_3444.

Organism-specific databases

EchoBASEiEB4144.
EcoGeneiEG20110. kefB.

Phylogenomic databases

eggNOGiENOG4105CKD. Bacteria.
COG0475. LUCA.
COG1226. LUCA.
HOGENOMiHOG000179076.
InParanoidiP45522.
KOiK11747.
OMAiRELMPVH.
PhylomeDBiP45522.

Enzyme and pathway databases

BioCyciEcoCyc:KEFB-MONOMER.
ECOL316407:JW3313-MONOMER.
MetaCyc:KEFB-MONOMER.

Miscellaneous databases

PROiP45522.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01412. K_H_efflux_KefB. 1 hit.
InterProiIPR006153. Cation/H_exchanger.
IPR004771. K/H_exchanger.
IPR020884. K_H_efflux_KefB.
IPR016040. NAD(P)-bd_dom.
IPR003148. RCK_N.
[Graphical view]
PfamiPF00999. Na_H_Exchanger. 1 hit.
PF02254. TrkA_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00932. 2a37. 1 hit.
PROSITEiPS51201. RCK_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKEFB_ECOLI
AccessioniPrimary (citable) accession number: P45522
Secondary accession number(s): Q2M716
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.