P45513 (DHAT_CITFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,3-propanediol dehydrogenase
EC=1.1.1.202

Alternative name(s):
1,3-propanediol oxidoreductase
3-hydroxypropionaldehyde reductase

Gene names

Name:

dhaT

Organism

Citrobacter freundii

Taxonomic identifier

546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate.
Catalytic activity	Propane-1,3-diol + NAD+ = 3-hydroxypropanal + NADH.
Cofactor	Iron.
Subunit structure	Homooctamer.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Iron NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	1,3-propanediol dehydrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	1,3-propanediol dehydrogenase		PRO_0000087841

Sequences

	Sequence		Length	Mass (Da)
	P45513 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 268F295DA83A8DCC	FASTA	387	41,482
	10 20 30 40 50 60 MSYRMFDYLV PNVNFFGPNA ISVVGERCKL LGGKKALLVT DKGLRAIKDG AVDKTLTHLR 70 80 90 100 110 120 EAGIDVVVFD GVEPNPKDTN VRDGLEVFRK EHCDIIVTVG GGSPHDCGKG IGIAATHEGD 130 140 150 160 170 180 LYSYAGIETL TNPLPPIVAV NTTAGTASEV TRHCVLTNTK TKVKFVIVSW RNLPSVSIND 190 200 210 220 230 240 PLLMLGKPAP LTAATGMDAL THAVEAYISK DANPVTDAAA IQAIRLIARN LRQAVALGSN 250 260 270 280 290 300 LKARENMAYA SLLAGMAFNN ANLGYVHAMA HQLGGLYDMP HGVANAVLLP HVARYNLIAN 310 320 330 340 350 360 PEKFADIAEF MGENTDGLST MDAAELAIHA IARLSADIGI PQHLRDLGVK EADFPYMAEM 370 380 ALKDGNAFSN PRKGNEKEIA EIFRQAF

References

[1]

"Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in Escherichia coli." Daniel R., Boenigk R., Gottschalk G. J. Bacteriol. 177:2151-2156(1995) [PubMed: 7721705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6750 / DSM 30040 / NCIB 8173 / M8BK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09771 Genomic DNA. Translation: AAB48848.1.
PIR	A56275.
3D structure databases
ProteinModelPortal	P45513.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAT_CITFR

Accession

Primary (citable) accession number: P45513

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 28, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program