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P45513 (DHAT_CITFR) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,3-propanediol dehydrogenase

EC=1.1.1.202
Alternative name(s):
1,3-propanediol oxidoreductase
3-hydroxypropionaldehyde reductase
Gene names
Name:dhaT
OrganismCitrobacter freundii
Taxonomic identifier546 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate.

Catalytic activity

Propane-1,3-diol + NAD+ = 3-hydroxypropanal + NADH.

Cofactor

Iron.

Subunit structure

Homooctamer.

Sequence similarities

Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
   LigandIron
NAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Molecular_function1,3-propanediol dehydrogenase activity

Inferred from direct assay Ref.1. Source: CACAO

metal ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3873871,3-propanediol dehydrogenase
PRO_0000087841

Sequences

Sequence LengthMass (Da)Tools
P45513 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 268F295DA83A8DCC

FASTA38741,482
        10         20         30         40         50         60 
MSYRMFDYLV PNVNFFGPNA ISVVGERCKL LGGKKALLVT DKGLRAIKDG AVDKTLTHLR 

        70         80         90        100        110        120 
EAGIDVVVFD GVEPNPKDTN VRDGLEVFRK EHCDIIVTVG GGSPHDCGKG IGIAATHEGD 

       130        140        150        160        170        180 
LYSYAGIETL TNPLPPIVAV NTTAGTASEV TRHCVLTNTK TKVKFVIVSW RNLPSVSIND 

       190        200        210        220        230        240 
PLLMLGKPAP LTAATGMDAL THAVEAYISK DANPVTDAAA IQAIRLIARN LRQAVALGSN 

       250        260        270        280        290        300 
LKARENMAYA SLLAGMAFNN ANLGYVHAMA HQLGGLYDMP HGVANAVLLP HVARYNLIAN 

       310        320        330        340        350        360 
PEKFADIAEF MGENTDGLST MDAAELAIHA IARLSADIGI PQHLRDLGVK EADFPYMAEM 

       370        380 
ALKDGNAFSN PRKGNEKEIA EIFRQAF 

« Hide

References

[1]"Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in Escherichia coli."
Daniel R., Boenigk R., Gottschalk G.
J. Bacteriol. 177:2151-2156(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 6750 / DSM 30040 / NCIB 8173 / M8BK.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09771 Genomic DNA. Translation: AAB48848.1.
PIRA56275.

3D structure databases

ProteinModelPortalP45513.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
[Graphical view]
PfamPF00465. Fe-ADH. 1 hit.
[Graphical view]
PROSITEPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHAT_CITFR
AccessionPrimary (citable) accession number: P45513
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 13, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families