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Protein

Probable autotransporter YfaL

Gene

yfaL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably an autotransporter.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell adhesion involved in biofilm formation Source: EcoCyc
  • response to UV Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG12850-MONOMER.

Protein family/group databases

MEROPSiU69.A11.
TCDBi1.B.12.1.5. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable autotransporter YfaL1 Publication
Cleaved into the following 2 chains:
Probable secreted autotransporter protein YfaL1 Publication
Probable autotransporter YfaL translocator1 Publication
Gene namesi
Name:yfaL
Synonyms:yfaF, yfaJ, yfaK
Ordered Locus Names:b2233, JW2227
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12850. yfaL.

Subcellular locationi

Probable autotransporter YfaL translocator :

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

Pathology & Biotechi

Biotechnological usei

The "passenger" domain (residues 29-785) can be replaced by other sequences which can be targeted to the cell surface (PubMed:22344647).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000000271329 – 1250Probable autotransporter YfaLAdd BLAST1222
ChainiPRO_000044002729 – 696Probable secreted autotransporter protein YfaLAdd BLAST668
ChainiPRO_0000440028697 – 1250Probable autotransporter YfaL translocatorAdd BLAST554

Post-translational modificationi

An approximately 170 kDa protein is detected in the outer membrane, while a C-terminal 55 kDa fragment is detected in whole cells. The full length putative autotransporter may be cleaved to release the mature protein from the outer membrane; Pefabloc SC, a Ser-Thr protease inhibitor prevents the appearance of the 55 kDa C--terminal fragment.1 Publication

Proteomic databases

PaxDbiP45508.
PRIDEiP45508.

Interactioni

Protein-protein interaction databases

BioGridi4262133. 267 interactors.
IntActiP45508. 6 interactors.
STRINGi316385.ECDH10B_2392.

Structurei

3D structure databases

ProteinModelPortaliP45508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati919 – 92012
Repeati921 – 9222; approximate2
Repeati923 – 92432
Repeati925 – 9264; approximate2
Repeati927 – 92852
Repeati929 – 93062
Repeati931 – 93272
Repeati933 – 93482
Repeati935 – 93692
Repeati937 – 938102
Repeati939 – 940112
Repeati941 – 942122
Repeati943 – 944132
Repeati945 – 946142
Repeati947 – 948152
Domaini980 – 1250AutotransporterPROSITE-ProRule annotationAdd BLAST271

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni919 – 94815 X 2 AA approximate tandem repeats of [DTPE]-PAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi920 – 952Pro-richPROSITE-ProRule annotationAdd BLAST33

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage. Finally, the mature protein remains tightly associated with the bacterium (Probable).Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4107GHC. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000122378.
KOiK07279.
PhylomeDBiP45508.

Family and domain databases

Gene3Di2.160.20.20. 2 hits.
InterProiView protein in InterPro
IPR005546. Autotransporte_beta.
IPR013425. Autotrns_rpt.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR003368. POMP_repeat.
PfamiView protein in Pfam
PF03797. Autotransporter. 1 hit.
PF02415. Chlam_PMP. 3 hits.
PF12951. PATR. 3 hits.
SMARTiView protein in SMART
SM00869. Autotransporter. 1 hit.
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
TIGR02601. autotrns_rpt. 1 hit.
TIGR01376. POMP_repeat. 2 hits.
PROSITEiView protein in PROSITE
PS51208. AUTOTRANSPORTER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIIFLRKEY LSLLPSMIAS LFSANGVAAV TDSCQGYDVK ASCQASRQSL
60 70 80 90 100
SGITQDWSIA DGQWLVFSDM TNNASGGAVF LQQGAEFSLL PENETGMTLF
110 120 130 140 150
ANNTVTGEYN NGGAIFAKEN STLNLTDVIF SGNVAGGYGG AIYSSGTNDT
160 170 180 190 200
GAVDLRVTNA MFRNNIANDG KGGAIYTINN DVYLSDVIFD NNQAYTSTSY
210 220 230 240 250
SDGDGGAIDV TDNNSDSKHP SGYTIVNNTA FTNNTAEGYG GAIYTNSVTA
260 270 280 290 300
PYLIDISVDD SYSQNGGVLV DENNSAAGYG DGPSSAAGGF MYLGLSEVTF
310 320 330 340 350
DIADGKTLVI GNTENDGAVD SIAGTGLITK TGSGDLVLNA DNNDFTGEMQ
360 370 380 390 400
IENGEVTLGR SNSLMNVGDT HCQDDPQDCY GLTIGSIDQY QNQAELNVGS
410 420 430 440 450
TQQTFVHALT GFQNGTLNID AGGNVTVNQG SFAGIIEGAG QLTIAQNGSY
460 470 480 490 500
VLAGAQSMAL TGDIVVDDGA VLSLEGDAAD LTALQDDPQS IVLNGGVLDL
510 520 530 540 550
SDFSTWQSGT SYNDGLEVSG SSGTVIGSQD VVDLAGGDNL HIGGDGKDGV
560 570 580 590 600
YVVVDASDGQ VSLANNNSYL GTTQIASGTL MVSDNSQLGD THYNRQVIFT
610 620 630 640 650
DKQQESVMEI TSDVDTRSDA AGHGRDIEMR ADGEVAVDAG VDTQWGALMA
660 670 680 690 700
DSSGQHQDEG STLTKTGAGT LELTASGTTQ SAVRVEEGTL KGDVADILPY
710 720 730 740 750
ASSLWVGDGA TFVTGADQDI QSIDAISSGT IDISDGTVLR LTGQDTSVAL
760 770 780 790 800
NASLFNGDGT LVNATDGVTL TGELNTNLET DSLTYLSNVT VNGNLTNTSG
810 820 830 840 850
AVSLQNGVAG DTLTVNGDYT GGGTLLLDSE LNGDDSVSDQ LVMNGNTAGN
860 870 880 890 900
TTVVVNSITG IGEPTSTGIK VVDFAADPTQ FQNNAQFSLA GSGYVNMGAY
910 920 930 940 950
DYTLVEDNND WYLRSQEVTP PSPPDPDPTP DPDPTPDPDP TPDPEPTPAY
960 970 980 990 1000
QPVLNAKVGG YLNNLRAANQ AFMMERRDHA GGDGQTLNLR VIGGDYHYTA
1010 1020 1030 1040 1050
AGQLAQHEDT STVQLSGDLF SGRWGTDGEW MLGIVGGYSD NQGDSRSNMT
1060 1070 1080 1090 1100
GTRADNQNHG YAVGLTSSWF QHGNQKQGAW LDSWLQYAWF SNDVSEQEDG
1110 1120 1130 1140 1150
TDHYHSSGII ASLEAGYQWL PGRGVVIEPQ AQVIYQGVQQ DDFTAANRAR
1160 1170 1180 1190 1200
VSQSQGDDIQ TRLGLHSEWR TAVHVIPTLD LNYYHDPHST EIEEDGSTIS
1210 1220 1230 1240 1250
DDAVKQRGEI KVGVTGNISQ RVSLRGSVAW QKGSDDFAQT AGFLSMTVKW
Length:1,250
Mass (Da):131,153
Last modified:November 1, 1997 - v2
Checksum:i17F98C05E299FC95
GO

Sequence cautioni

The sequence K02672 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28 – 30AAV → RGRS in K02672 (PubMed:6087316).Curated3
Sequence conflicti40K → Q in K02672 (PubMed:6087316).Curated1
Sequence conflicti65 – 66LV → PG in K02672 (PubMed:6087316).Curated2
Sequence conflicti431S → Q in K02672 (PubMed:6087316).Curated1
Sequence conflicti433 – 434AG → SA in K02672 (PubMed:6087316).Curated2
Sequence conflicti478A → R in K02672 (PubMed:6087316).Curated1
Sequence conflicti773E → S in K02672 (PubMed:6087316).Curated1
Sequence conflicti853V → M in K02672 (PubMed:6087316).Curated1
Sequence conflicti923 – 924PP → AT in K02672 (PubMed:6087316).Curated2
Sequence conflicti948 – 994PAYQP…RVIGG → LLTSRC in AAA74094 (Ref. 5) CuratedAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75293.1.
AP009048 Genomic DNA. Translation: BAA16050.2.
K02672 Genomic DNA. No translation available.
U30459 Genomic DNA. Translation: AAA74094.1.
Y00544 Genomic DNA. No translation available.
PIRiG64993.
RefSeqiNP_416736.1. NC_000913.3.
WP_001220077.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75293; AAC75293; b2233.
BAA16050; BAA16050; BAA16050.
GeneIDi946595.
KEGGiecj:JW2227.
eco:b2233.
PATRICifig|511145.12.peg.2322.

Similar proteinsi

Entry informationi

Entry nameiYFAL_ECOLI
AccessioniPrimary (citable) accession number: P45508
Secondary accession number(s): P39441
, P45506, P45507, P76468, P77487
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: August 30, 2017
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene