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P45487 (BIOF_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
8-amino-7-oxononanoate synthase
Short name=AONS
EC=2.3.1.47
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name=7-KAP synthase
Short name=KAPA synthase
8-amino-7-ketopelargonate synthase
Alpha-oxoamine synthase
Gene names
Ordered Locus Names:ML1217
ORF Names:B1170_C2_196
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium ›

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide By similarity.

Catalytic activity

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   LigandPyridoxal phosphate
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function8-amino-7-oxononanoate synthase activity

Inferred from electronic annotation. Source: EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3853858-amino-7-oxononanoate synthase
PRO_0000163817

Regions

Region105 – 1062Pyridoxal phosphate binding By similarity
Region201 – 2044Pyridoxal phosphate binding By similarity
Region232 – 2354Pyridoxal phosphate binding By similarity

Sites

Binding site271Substrate By similarity
Binding site1301Substrate By similarity
Binding site1761Pyridoxal phosphate By similarity
Binding site3451Substrate By similarity

Amino acid modifications

Modified residue2351N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P45487 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0CA37DD1FACC44AA

FASTA38539,831
        10         20         30         40         50         60 
MKVPIETSPL AWLEAVEQQR RGAGLRRSLR PRSAVATELD LASNDYLGLS QHPDVIDGGV 

        70         80         90        100        110        120 
AALRVWGAGA TGSRLVTGDT ILHHELECEL AEFVGACVGL LFSSGYAANL GAVVGLSGPG 

       130        140        150        160        170        180 
SLIVSDAYSH ASLVDACRLS RARVVVTPHC DVDAVDTALR SCHEERAVVV TESVFSADGV 

       190        200        210        220        230        240 
LAPVSELHDV CRRHGALLLV DEAHGLGVRG GGRGLVYEVG LAGAPDVVIT TTMSKALGSQ 

       250        260        270        280        290        300 
GGAVLGSSAV RAHLINTARP FIFDTGLAPA AVGAARAALQ ILKAETWRPE AVLQHARTLA 

       310        320        330        340        350        360 
KICDLSELPQ SAVVSVVLGD PEVALAAAIA CLDAGVRVGC FRPPTVPAGT SRLRLTAHAS 

       370        380 
LDSAKLEVAR RVLTDVLGGC CVARR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U00010 Genomic DNA. Translation: AAA17066.1.
AL583921 Genomic DNA. Translation: CAC31598.1.
PIRS72702.
RefSeqNP_301881.1. NC_002677.1.

3D structure databases

ProteinModelPortalP45487.
ModBaseSearch...

Protein-protein interaction databases

STRING272631.ML1217.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC31598; CAC31598; CAC31598.
GeneID910326.
KEGGmle:ML1217.
PATRIC18054592. VBIMycLep78757_2236.

Organism-specific databases

LepromaML1217.
CMRSearch...

Phylogenomic databases

eggNOGCOG0156.
HOGENOMHOG000221021.
KOK00652.
OMAVCVESVY.
ProtClustDBPRK05958.

Enzyme and pathway databases

UniPathwayUPA00078.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBIOF_MYCLE
AccessionPrimary (citable) accession number: P45487
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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