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Protein

ATP-dependent dethiobiotin synthetase BioD

Gene

bioD

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring.UniRule annotation

Catalytic activityi

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16Magnesium 2UniRule annotation1
Binding sitei41SubstrateUniRule annotation1
Metal bindingi49Magnesium 2UniRule annotation1
Binding sitei49ATPUniRule annotation1
Metal bindingi108Magnesium 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 111ATPUniRule annotation4
Nucleotide bindingi197 – 199ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processBiotin biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-1328-MONOMER
UniPathwayiUPA00078; UER00161

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent dethiobiotin synthetase BioDUniRule annotation (EC:6.3.3.3UniRule annotation)
Alternative name(s):
DTB synthetaseUniRule annotation
Short name:
DTBSUniRule annotation
Dethiobiotin synthaseUniRule annotation
Gene namesi
Name:bioDUniRule annotation
Ordered Locus Names:ML1218
ORF Names:B1170_C1_159
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1218

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001879761 – 226ATP-dependent dethiobiotin synthetase BioDAdd BLAST226

Proteomic databases

PRIDEiP45486

Interactioni

Protein-protein interaction databases

STRINGi272631.ML1218

Structurei

3D structure databases

ProteinModelPortaliP45486
SMRiP45486
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the dethiobiotin synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107WHG Bacteria
COG0132 LUCA
HOGENOMiHOG000275033
KOiK01935
OMAiDYWKPIQ
OrthoDBiPOG091H004W

Family and domain databases

HAMAPiMF_00336 BioD, 1 hit
InterProiView protein in InterPro
IPR004472 DTB_synth_BioD
IPR027417 P-loop_NTPase
PANTHERiPTHR43210 PTHR43210, 1 hit
PIRSFiPIRSF006755 DTB_synth, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00347 bioD, 1 hit

Sequencei

Sequence statusi: Complete.

P45486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVVVVTGTD TGVGKTVACA ALACHARQAG IEVAVCKPVQ TGTQIGDDDL
60 70 80 90 100
AEVARLSGVT ELTGLVRYPQ PLAPAAAAEH AGMALPTREQ LLELIAGLDR
110 120 130 140 150
PGRLILVEGA GGLLVELADA SATLRDLAVE LGALALVTVS VELGTLNHTA
160 170 180 190 200
LTLEALTTRG VACAGLVIGS WTGRPGAVQI SNRSALARLA PMRATLPAGA
210 220
GSMDATDFAA MSAAAFDRDW VTTLVH
Length:226
Mass (Da):22,923
Last modified:April 27, 2001 - v2
Checksum:iC1A1B03C0C728E22
GO

Sequence cautioni

The sequence AAA17057 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00010 Genomic DNA Translation: AAA17057.1 Different initiation.
AL583921 Genomic DNA Translation: CAC31599.1
PIRiD87061
S72693
RefSeqiNP_301882.1, NC_002677.1
WP_010908203.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC31599; CAC31599; CAC31599
GeneIDi910322
KEGGimle:ML1218
PATRICifig|272631.5.peg.2237

Similar proteinsi

Entry informationi

Entry nameiBIOD_MYCLE
AccessioniPrimary (citable) accession number: P45486
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health