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P45480

- GSHB_NOSS1

UniProt

P45480 - GSHB_NOSS1

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Protein

Glutathione synthetase

Gene
gshB, gsh-II, all3859
Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi288 – 2881Magnesium or manganese By similarity
Metal bindingi290 – 2901Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi159 – 21557ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutathione synthase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshB
Synonyms:gsh-II
Ordered Locus Names:all3859
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Glutathione synthetaseUniRule annotationPRO_0000197452Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi103690.all3859.

Structurei

3D structure databases

ProteinModelPortaliP45480.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 317185ATP-graspAdd
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiERERQIC.
OrthoDBiEOG6WMHWB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45480-1 [UniParc]FASTAAdd to Basket

« Hide

MKLAFIIDPI HQLDPCHDTS VALMEAAQIL GHEVWVTQAN WLSVVDSKAW    50
AILQQVELVP VQLIDGRWVA ASPWYTLNTR SFSSLETMDA VFMRTDPPVN 100
DAYLYATYVL DYVDQRKTLV INNPNGIRGA NEKMYALQFT KAIPETIVSA 150
DKDFIRQFVE AKGATVLKPL GNKAGEGILF LQAGDRNFNS IVELSTQQGR 200
LPVMVQTYLP EAKEGDKRII LLNGEPIGAL NRLASGSDFR NNMATGGTVA 250
KTEITPREEE ICSQIAANLR QDGLIFVGID VIGGYLTEVN VTSPTGIREI 300
DRLDGTRLAH QVIQWVEKNL QIQN 324
Length:324
Mass (Da):35,953
Last modified:January 23, 2002 - v2
Checksum:i03C6E7E8481DD8D1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501W → C in AAA85527. 1 Publication
Sequence conflicti243 – 2431M → V in CAA83242. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14408 Genomic DNA. Translation: AAA85527.1.
BA000019 Genomic DNA. Translation: BAB75558.1.
Z31371 Genomic DNA. Translation: CAA83242.1.
PIRiAD2288.
JC4291.
RefSeqiNP_487899.1. NC_003272.1.
WP_010998000.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB75558; BAB75558; BAB75558.
GeneIDi1107457.
KEGGiana:all3859.
PATRICi22778204. VBINosSp37423_4556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14408 Genomic DNA. Translation: AAA85527.1 .
BA000019 Genomic DNA. Translation: BAB75558.1 .
Z31371 Genomic DNA. Translation: CAA83242.1 .
PIRi AD2288.
JC4291.
RefSeqi NP_487899.1. NC_003272.1.
WP_010998000.1. NC_003272.1.

3D structure databases

ProteinModelPortali P45480.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 103690.all3859.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB75558 ; BAB75558 ; BAB75558 .
GeneIDi 1107457.
KEGGi ana:all3859.
PATRICi 22778204. VBINosSp37423_4556.

Phylogenomic databases

eggNOGi COG0189.
HOGENOMi HOG000265022.
KOi K01920.
OMAi ERERQIC.
OrthoDBi EOG6WMHWB.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPi MF_00162. GSH_S.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01380. glut_syn. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence of ftsZ and flanking regions from the cyanobacterium Anabaena PCC 7120."
    Doherty H.M., Adams D.G.
    Gene 163:93-96(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.
  3. "Analysis of genes encoding the cell division protein FtsZ and a glutathione synthetase homologue in the cyanobacterium Anabaena sp. PCC 7120."
    Zhang C.C., Huguenin S., Friry A.
    Res. Microbiol. 146:445-455(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-324.

Entry informationi

Entry nameiGSHB_NOSS1
AccessioniPrimary (citable) accession number: P45480
Secondary accession number(s): Q43879
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2002
Last modified: September 3, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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