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P45480 (GSHB_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Synonyms:gsh-II
Ordered Locus Names:all3859
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Magnesium
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutathione synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197452

Regions

Domain133 – 317185ATP-grasp
Nucleotide binding159 – 21557ATP By similarity

Sites

Metal binding2881Magnesium or manganese By similarity
Metal binding2901Magnesium or manganese By similarity

Experimental info

Sequence conflict501W → C in AAA85527. Ref.1
Sequence conflict2431M → V in CAA83242. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P45480 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 03C6E7E8481DD8D1

FASTA32435,953
        10         20         30         40         50         60 
MKLAFIIDPI HQLDPCHDTS VALMEAAQIL GHEVWVTQAN WLSVVDSKAW AILQQVELVP 

        70         80         90        100        110        120 
VQLIDGRWVA ASPWYTLNTR SFSSLETMDA VFMRTDPPVN DAYLYATYVL DYVDQRKTLV 

       130        140        150        160        170        180 
INNPNGIRGA NEKMYALQFT KAIPETIVSA DKDFIRQFVE AKGATVLKPL GNKAGEGILF 

       190        200        210        220        230        240 
LQAGDRNFNS IVELSTQQGR LPVMVQTYLP EAKEGDKRII LLNGEPIGAL NRLASGSDFR 

       250        260        270        280        290        300 
NNMATGGTVA KTEITPREEE ICSQIAANLR QDGLIFVGID VIGGYLTEVN VTSPTGIREI 

       310        320 
DRLDGTRLAH QVIQWVEKNL QIQN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence of ftsZ and flanking regions from the cyanobacterium Anabaena PCC 7120."
Doherty H.M., Adams D.G.
Gene 163:93-96(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.
[3]"Analysis of genes encoding the cell division protein FtsZ and a glutathione synthetase homologue in the cyanobacterium Anabaena sp. PCC 7120."
Zhang C.C., Huguenin S., Friry A.
Res. Microbiol. 146:445-455(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-324.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14408 Genomic DNA. Translation: AAA85527.1.
BA000019 Genomic DNA. Translation: BAB75558.1.
Z31371 Genomic DNA. Translation: CAA83242.1.
PIRAD2288.
JC4291.
RefSeqNP_487899.1. NC_003272.1.

3D structure databases

ProteinModelPortalP45480.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.all3859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB75558; BAB75558; BAB75558.
GeneID1107457.
KEGGana:all3859.
PATRIC22778204. VBINosSp37423_4556.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAERERQIC.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_NOSS1
AccessionPrimary (citable) accession number: P45480
Secondary accession number(s): Q43879
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2002
Last modified: May 14, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways