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P45480

- GSHB_NOSS1

UniProt

P45480 - GSHB_NOSS1

Protein

Glutathione synthetase

Gene

gshB

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi288 – 2881Magnesium or manganeseUniRule annotation
    Metal bindingi290 – 2901Magnesium or manganeseUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi159 – 21557ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutathione synthase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshBUniRule annotation
    Synonyms:gsh-II
    Ordered Locus Names:all3859
    OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
    Taxonomic identifieri103690 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000002483: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324Glutathione synthetasePRO_0000197452Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi103690.all3859.

    Structurei

    3D structure databases

    ProteinModelPortaliP45480.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini133 – 317185ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic GSH synthase family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0189.
    HOGENOMiHOG000265022.
    KOiK01920.
    OMAiERERQIC.
    OrthoDBiEOG6WMHWB.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_00162. GSH_S.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01380. glut_syn. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45480-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLAFIIDPI HQLDPCHDTS VALMEAAQIL GHEVWVTQAN WLSVVDSKAW    50
    AILQQVELVP VQLIDGRWVA ASPWYTLNTR SFSSLETMDA VFMRTDPPVN 100
    DAYLYATYVL DYVDQRKTLV INNPNGIRGA NEKMYALQFT KAIPETIVSA 150
    DKDFIRQFVE AKGATVLKPL GNKAGEGILF LQAGDRNFNS IVELSTQQGR 200
    LPVMVQTYLP EAKEGDKRII LLNGEPIGAL NRLASGSDFR NNMATGGTVA 250
    KTEITPREEE ICSQIAANLR QDGLIFVGID VIGGYLTEVN VTSPTGIREI 300
    DRLDGTRLAH QVIQWVEKNL QIQN 324
    Length:324
    Mass (Da):35,953
    Last modified:January 23, 2002 - v2
    Checksum:i03C6E7E8481DD8D1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 501W → C in AAA85527. (PubMed:7557485)Curated
    Sequence conflicti243 – 2431M → V in CAA83242. (PubMed:8525061)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14408 Genomic DNA. Translation: AAA85527.1.
    BA000019 Genomic DNA. Translation: BAB75558.1.
    Z31371 Genomic DNA. Translation: CAA83242.1.
    PIRiAD2288.
    JC4291.
    RefSeqiNP_487899.1. NC_003272.1.
    WP_010998000.1. NC_003272.1.

    Genome annotation databases

    EnsemblBacteriaiBAB75558; BAB75558; BAB75558.
    GeneIDi1107457.
    KEGGiana:all3859.
    PATRICi22778204. VBINosSp37423_4556.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14408 Genomic DNA. Translation: AAA85527.1 .
    BA000019 Genomic DNA. Translation: BAB75558.1 .
    Z31371 Genomic DNA. Translation: CAA83242.1 .
    PIRi AD2288.
    JC4291.
    RefSeqi NP_487899.1. NC_003272.1.
    WP_010998000.1. NC_003272.1.

    3D structure databases

    ProteinModelPortali P45480.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 103690.all3859.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB75558 ; BAB75558 ; BAB75558 .
    GeneIDi 1107457.
    KEGGi ana:all3859.
    PATRICi 22778204. VBINosSp37423_4556.

    Phylogenomic databases

    eggNOGi COG0189.
    HOGENOMi HOG000265022.
    KOi K01920.
    OMAi ERERQIC.
    OrthoDBi EOG6WMHWB.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPi MF_00162. GSH_S.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01380. glut_syn. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence of ftsZ and flanking regions from the cyanobacterium Anabaena PCC 7120."
      Doherty H.M., Adams D.G.
      Gene 163:93-96(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7120 / UTEX 2576.
    3. "Analysis of genes encoding the cell division protein FtsZ and a glutathione synthetase homologue in the cyanobacterium Anabaena sp. PCC 7120."
      Zhang C.C., Huguenin S., Friry A.
      Res. Microbiol. 146:445-455(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-324.

    Entry informationi

    Entry nameiGSHB_NOSS1
    AccessioniPrimary (citable) accession number: P45480
    Secondary accession number(s): Q43879
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3