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Protein

Palmitoyl-protein thioesterase 1

Gene

PPT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.1 Publication

Catalytic activityi

Palmitoyl-[protein] + H2O = palmitate + [protein].1 Publication

Kineticsi

  1. KM=5.7 µM for palmitoyl-CoA1 Publication
  2. KM=2.3 µM for stearoyl-CoA1 Publication
  3. KM=2.8 µM for oleoyl-CoA1 Publication
  4. KM=7.5 µM for myristoyl-CoA1 Publication
  1. Vmax=5.7 nmol/min/mg enzyme with palmitoyl-CoA as substrate1 Publication
  2. Vmax=1.8 nmol/min/mg enzyme with stearoyl-CoA as substrate1 Publication
  3. Vmax=1.7 nmol/min/mg enzyme with oleoyl-CoA as substrate1 Publication
  4. Vmax=1.9 nmol/min/mg enzyme with myristoyl-CoA as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1151 Publication1
Active sitei2331 Publication1
Active sitei2891 Publication1

GO - Molecular functioni

  • palmitoyl-(protein) hydrolase activity Source: UniProtKB
  • palmitoyl-CoA hydrolase activity Source: UniProtKB

GO - Biological processi

  • brain development Source: UniProtKB
  • chemical synaptic transmission Source: GO_Central
  • cofactor metabolic process Source: UniProtKB
  • cofactor transport Source: UniProtKB
  • lipid catabolic process Source: UniProtKB
  • lysosomal lumen acidification Source: UniProtKB
  • membrane raft organization Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: UniProtKB
  • nervous system development Source: UniProtKB
  • pinocytosis Source: AgBase
  • positive regulation of pinocytosis Source: UniProtKB
  • positive regulation of receptor-mediated endocytosis Source: UniProtKB
  • protein depalmitoylation Source: UniProtKB
  • protein transport Source: UniProtKB
  • receptor-mediated endocytosis Source: AgBase

Keywordsi

Molecular functionHydrolase

Protein family/group databases

ESTHERibovin-ppt Palmitoyl-protein_thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyl-protein thioesterase 1 (EC:3.1.2.22)
Short name:
PPT-1
Alternative name(s):
Palmitoyl-protein hydrolase 1
Gene namesi
Name:PPT1
Synonyms:CLN1, PPT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000002554928 – 306Palmitoyl-protein thioesterase 1Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 46By similarity
Disulfide bondi96 ↔ 128By similarity
Disulfide bondi152 ↔ 160By similarity
Glycosylationi197N-linked (GlcNAc...) asparagine1
Glycosylationi212N-linked (GlcNAc...) asparagine1
Glycosylationi232N-linked (GlcNAc...) asparagine1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP45478
PRIDEiP45478

Expressioni

Tissue specificityi

Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle.1 Publication

Interactioni

Subunit structurei

Interacts with CLN5, ATP5F1A and ATP5F1B.By similarity

Protein-protein interaction databases

IntActiP45478, 2 interactors
STRINGi9913.ENSBTAP00000017780

Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 32Combined sources3
Beta strandi35 – 38Combined sources4
Turni48 – 51Combined sources4
Helixi52 – 61Combined sources10
Beta strandi67 – 69Combined sources3
Beta strandi73 – 75Combined sources3
Helixi76 – 85Combined sources10
Helixi88 – 100Combined sources13
Helixi103 – 105Combined sources3
Beta strandi109 – 114Combined sources6
Helixi117 – 127Combined sources11
Beta strandi133 – 140Combined sources8
Helixi158 – 166Combined sources9
Helixi168 – 171Combined sources4
Helixi174 – 179Combined sources6
Helixi182 – 185Combined sources4
Helixi192 – 198Combined sources7
Helixi202 – 205Combined sources4
Turni206 – 209Combined sources4
Helixi213 – 220Combined sources8
Beta strandi222 – 230Combined sources9
Beta strandi234 – 238Combined sources5
Helixi239 – 243Combined sources5
Helixi258 – 260Combined sources3
Helixi262 – 265Combined sources4
Beta strandi268 – 270Combined sources3
Helixi271 – 276Combined sources6
Beta strandi280 – 288Combined sources9
Helixi294 – 300Combined sources7
Helixi302 – 304Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EH5X-ray2.50A28-306[»]
1EI9X-ray2.25A28-306[»]
1EXWX-ray2.40A28-306[»]
ProteinModelPortaliP45478
SMRiP45478
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45478

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2541 Eukaryota
COG1075 LUCA
HOGENOMiHOG000199232
HOVERGENiHBG018186
InParanoidiP45478
KOiK01074

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002472 Palm_thioest
IPR030294 PPT1
PANTHERiPTHR11247:SF8 PTHR11247:SF8, 1 hit
PfamiView protein in Pfam
PF02089 Palm_thioest, 1 hit
PRINTSiPR00414 PPTHIESTRASE
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSCLWLL ALAFLLGSCA SLALGHLDPP APLPLVIWHG MGDSCCNPLS
60 70 80 90 100
MGAIKKMVEK KIPGIHVLSL EIGKTLREDV ENSFFLNVNS QVTTVCQILA
110 120 130 140 150
KDPKLQQGYN AMGFSQGGQF LRAVAQRCPS PPMVNLISVG GQHQGVFGLP
160 170 180 190 200
RCPGESSHIC DFIRKTLNAG AYNKAIQERL VQAEYWHDPI REDIYRNHSI
210 220 230 240 250
FLADINQERG VNESYKKNLM ALKKFVMVKF LNDTIVDPVD SEWFGFYRSG
260 270 280 290 300
QAKETIPLQE STLYTQDRLG LKAMDKAGQL VFLALEGDHL QLSEEWFYAH

IIPFLE
Length:306
Mass (Da):34,142
Last modified:November 1, 1995 - v1
Checksum:iF9893D3CE8099D51
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti72I → F in clone BOVPTT-25. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34261 mRNA Translation: AAA59357.1
BC134479 mRNA Translation: AAI34480.1
PIRiB54717
RefSeqiNP_776579.1, NM_174154.2
UniGeneiBt.5019

Genome annotation databases

GeneIDi281421
KEGGibta:281421

Similar proteinsi

Entry informationi

Entry nameiPPT1_BOVIN
AccessioniPrimary (citable) accession number: P45478
Secondary accession number(s): A7YWB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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