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Protein

Palmitoyl-protein thioesterase 1

Gene

PPT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.1 Publication

Catalytic activityi

Palmitoyl-[protein] + H2O = palmitate + [protein].1 Publication

Kineticsi

  1. KM=5.7 µM for palmitoyl-CoA1 Publication
  2. KM=2.3 µM for stearoyl-CoA1 Publication
  3. KM=2.8 µM for oleoyl-CoA1 Publication
  4. KM=7.5 µM for myristoyl-CoA1 Publication
  1. Vmax=5.7 nmol/min/mg enzyme with palmitoyl-CoA as substrate1 Publication
  2. Vmax=1.8 nmol/min/mg enzyme with stearoyl-CoA as substrate1 Publication
  3. Vmax=1.7 nmol/min/mg enzyme with oleoyl-CoA as substrate1 Publication
  4. Vmax=1.9 nmol/min/mg enzyme with myristoyl-CoA as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1151 Publication1
Active sitei2331 Publication1
Active sitei2891 Publication1

GO - Molecular functioni

  • palmitoyl-(protein) hydrolase activity Source: UniProtKB
  • palmitoyl-CoA hydrolase activity Source: UniProtKB

GO - Biological processi

  • brain development Source: UniProtKB
  • chemical synaptic transmission Source: GO_Central
  • cofactor metabolic process Source: UniProtKB
  • cofactor transport Source: UniProtKB
  • lipid catabolic process Source: UniProtKB
  • lysosomal lumen acidification Source: UniProtKB
  • membrane raft organization Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: UniProtKB
  • nervous system development Source: UniProtKB
  • pinocytosis Source: AgBase
  • positive regulation of pinocytosis Source: UniProtKB
  • positive regulation of receptor-mediated endocytosis Source: UniProtKB
  • protein depalmitoylation Source: UniProtKB
  • protein transport Source: UniProtKB
  • receptor-mediated endocytosis Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

ESTHERibovin-ppt. Palmitoyl-protein_thioesterase.

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyl-protein thioesterase 1 (EC:3.1.2.22)
Short name:
PPT-1
Alternative name(s):
Palmitoyl-protein hydrolase 1
Gene namesi
Name:PPT1
Synonyms:CLN1, PPT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Lysosome 1 Publication
  • Secreted 1 Publication

GO - Cellular componenti

  • axon Source: UniProtKB
  • cytosol Source: UniProtKB
  • extracellular region Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • lysosome Source: UniProtKB
  • membrane raft Source: UniProtKB
  • nucleus Source: UniProtKB
  • synaptic vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000002554928 – 306Palmitoyl-protein thioesterase 1Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 46By similarity
Disulfide bondi96 ↔ 128By similarity
Disulfide bondi152 ↔ 160By similarity
Glycosylationi197N-linked (GlcNAc...)1
Glycosylationi212N-linked (GlcNAc...)1
Glycosylationi232N-linked (GlcNAc...)1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP45478.
PRIDEiP45478.

Expressioni

Tissue specificityi

Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle.1 Publication

Interactioni

Subunit structurei

Interacts with CLN5, ATP5A1 and ATP5B.By similarity

Protein-protein interaction databases

IntActiP45478. 2 interactors.
STRINGi9913.ENSBTAP00000017780.

Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 32Combined sources3
Beta strandi35 – 38Combined sources4
Turni48 – 51Combined sources4
Helixi52 – 61Combined sources10
Beta strandi67 – 69Combined sources3
Beta strandi73 – 75Combined sources3
Helixi76 – 85Combined sources10
Helixi88 – 100Combined sources13
Helixi103 – 105Combined sources3
Beta strandi109 – 114Combined sources6
Helixi117 – 127Combined sources11
Beta strandi133 – 140Combined sources8
Helixi158 – 166Combined sources9
Helixi168 – 171Combined sources4
Helixi174 – 179Combined sources6
Helixi182 – 185Combined sources4
Helixi192 – 198Combined sources7
Helixi202 – 205Combined sources4
Turni206 – 209Combined sources4
Helixi213 – 220Combined sources8
Beta strandi222 – 230Combined sources9
Beta strandi234 – 238Combined sources5
Helixi239 – 243Combined sources5
Helixi258 – 260Combined sources3
Helixi262 – 265Combined sources4
Beta strandi268 – 270Combined sources3
Helixi271 – 276Combined sources6
Beta strandi280 – 288Combined sources9
Helixi294 – 300Combined sources7
Helixi302 – 304Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EH5X-ray2.50A28-306[»]
1EI9X-ray2.25A28-306[»]
1EXWX-ray2.40A28-306[»]
ProteinModelPortaliP45478.
SMRiP45478.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45478.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2541. Eukaryota.
COG1075. LUCA.
HOGENOMiHOG000199232.
HOVERGENiHBG018186.
InParanoidiP45478.
KOiK01074.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002472. Palm_thioest.
IPR030294. PPT1.
[Graphical view]
PANTHERiPTHR11247:SF8. PTHR11247:SF8. 1 hit.
PfamiPF02089. Palm_thioest. 1 hit.
[Graphical view]
PRINTSiPR00414. PPTHIESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSCLWLL ALAFLLGSCA SLALGHLDPP APLPLVIWHG MGDSCCNPLS
60 70 80 90 100
MGAIKKMVEK KIPGIHVLSL EIGKTLREDV ENSFFLNVNS QVTTVCQILA
110 120 130 140 150
KDPKLQQGYN AMGFSQGGQF LRAVAQRCPS PPMVNLISVG GQHQGVFGLP
160 170 180 190 200
RCPGESSHIC DFIRKTLNAG AYNKAIQERL VQAEYWHDPI REDIYRNHSI
210 220 230 240 250
FLADINQERG VNESYKKNLM ALKKFVMVKF LNDTIVDPVD SEWFGFYRSG
260 270 280 290 300
QAKETIPLQE STLYTQDRLG LKAMDKAGQL VFLALEGDHL QLSEEWFYAH

IIPFLE
Length:306
Mass (Da):34,142
Last modified:November 1, 1995 - v1
Checksum:iF9893D3CE8099D51
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti72I → F in clone BOVPTT-25. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34261 mRNA. Translation: AAA59357.1.
BC134479 mRNA. Translation: AAI34480.1.
PIRiB54717.
RefSeqiNP_776579.1. NM_174154.2.
UniGeneiBt.5019.

Genome annotation databases

GeneIDi281421.
KEGGibta:281421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34261 mRNA. Translation: AAA59357.1.
BC134479 mRNA. Translation: AAI34480.1.
PIRiB54717.
RefSeqiNP_776579.1. NM_174154.2.
UniGeneiBt.5019.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EH5X-ray2.50A28-306[»]
1EI9X-ray2.25A28-306[»]
1EXWX-ray2.40A28-306[»]
ProteinModelPortaliP45478.
SMRiP45478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP45478. 2 interactors.
STRINGi9913.ENSBTAP00000017780.

Protein family/group databases

ESTHERibovin-ppt. Palmitoyl-protein_thioesterase.

Proteomic databases

PaxDbiP45478.
PRIDEiP45478.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281421.
KEGGibta:281421.

Organism-specific databases

CTDi5538.

Phylogenomic databases

eggNOGiKOG2541. Eukaryota.
COG1075. LUCA.
HOGENOMiHOG000199232.
HOVERGENiHBG018186.
InParanoidiP45478.
KOiK01074.

Miscellaneous databases

EvolutionaryTraceiP45478.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002472. Palm_thioest.
IPR030294. PPT1.
[Graphical view]
PANTHERiPTHR11247:SF8. PTHR11247:SF8. 1 hit.
PfamiPF02089. Palm_thioest. 1 hit.
[Graphical view]
PRINTSiPR00414. PPTHIESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPT1_BOVIN
AccessioniPrimary (citable) accession number: P45478
Secondary accession number(s): A7YWB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.