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Protein

Penicillin-binding protein activator LpoA

Gene

lpoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulator of peptidoglycan synthesis that is essential for the function of penicillin-binding protein 1A (PBP1a). Stimulates transpeptidase activity of PBP1a in vitro.2 Publications

GO - Molecular functioni

GO - Biological processi

  • peptidoglycan biosynthetic process Source: UniProtKB
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Biological processCell shape, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7642-MONOMER
MetaCyc:G7642-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein activator LpoA
Short name:
PBP activator LpoA
Alternative name(s):
Lipoprotein activator of PBP from the outer membrane A
Gene namesi
Name:lpoA
Synonyms:yraM
Ordered Locus Names:b3147, JW3116
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12778 lpoA

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • periplasmic side of cell outer membrane Source: UniProtKB
  • periplasmic space Source: InterPro

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Absence of both LpoA and LpoB leads to a decrease in peptide cross-linking and to cell lysis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000016945227 – 678Penicillin-binding protein activator LpoAAdd BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi27N-palmitoyl cysteineSequence analysis1
Lipidationi27S-diacylglycerol cysteineSequence analysis1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP45464
PaxDbiP45464
PRIDEiP45464

Interactioni

Subunit structurei

Interacts with PBP1a.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi426198957 interactors.
DIPiDIP-12897N
IntActiP45464 4 interactors.
STRINGi316385.ECDH10B_3320

Structurei

Secondary structure

1678
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 40Combined sources4
Helixi47 – 54Combined sources8
Helixi59 – 76Combined sources18
Helixi80 – 86Combined sources7
Helixi95 – 109Combined sources15
Turni110 – 113Combined sources4
Helixi115 – 122Combined sources8
Turni127 – 129Combined sources3
Helixi132 – 139Combined sources8
Helixi142 – 145Combined sources4
Helixi152 – 161Combined sources10
Turni162 – 164Combined sources3
Helixi167 – 181Combined sources15
Helixi186 – 189Combined sources4
Helixi200 – 214Combined sources15
Helixi218 – 231Combined sources14
Helixi236 – 238Combined sources3
Turni243 – 245Combined sources3
Helixi246 – 248Combined sources3
Beta strandi250 – 252Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MHKNMR-A28-256[»]
ProteinModelPortaliP45464
SMRiP45464
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpoA family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E1C Bacteria
COG3107 LUCA
HOGENOMiHOG000125217
InParanoidiP45464
KOiK07121
OMAiMRLYAMG

Family and domain databases

Gene3Di1.25.40.101 hit
HAMAPiMF_01890 LpoA, 1 hit
InterProiView protein in InterPro
IPR007443 LpoA
IPR008939 Lytic_TGlycosylase_superhlx_U
IPR028082 Peripla_BP_I
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR38038 PTHR38038, 1 hit
PfamiView protein in Pfam
PF04348 LppC, 1 hit
SUPFAMiSSF48435 SSF48435, 1 hit
SSF53822 SSF53822, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPSTFSRLK AARCLPVVLA ALIFAGCGTH TPDQSTAYMQ GTAQADSAFY
60 70 80 90 100
LQQMQQSSDD TRINWQLLAI RALVKEGKTG QAVELFNQLP QELNDAQRRE
110 120 130 140 150
KTLLAVEIKL AQKDFAGAQN LLAKITPADL EQNQQARYWQ AKIDASQGRP
160 170 180 190 200
SIDLLRALIA QEPLLGAKEK QQNIDATWQA LSSMTQEQAN TLVINADENI
210 220 230 240 250
LQGWLDLQRV WFDNRNDPDM MKAGIADWQK RYPNNPGAKM LPTQLVNVKA
260 270 280 290 300
FKPASTNKIA LLLPLNGQAA VFGRTIQQGF EAAKNIGTQP VAAQVAAAPA
310 320 330 340 350
ADVAEQPQPQ TVDGVASPAQ ASVSDLTGEQ PAAQPVPVSA PATSTAAVSA
360 370 380 390 400
PANPSAELKI YDTSSQPLSQ ILSQVQQDGA SIVVGPLLKN NVEELLKSNT
410 420 430 440 450
PLNVLALNQP ENIENRVNIC YFALSPEDEA RDAARHIRDQ GKQAPLVLIP
460 470 480 490 500
RSSLGDRVAN AFAQEWQKLG GGTVLQQKFG STSELRAGVN GGSGIALTGS
510 520 530 540 550
PITLRATTDS GMTTNNPTLQ TTPTDDQFTN NGGRVDAVYI VATPGEIAFI
560 570 580 590 600
KPMIAMRNGS QSGATLYASS RSAQGTAGPD FRLEMEGLQY SEIPMLAGGN
610 620 630 640 650
LPLMQQALSA VNNDYSLARM YAMGVDAWSL ANHFSQMRQV QGFEINGNTG
660 670
SLTANPDCVI NRNLSWLQYQ QGQVVPVS
Length:678
Mass (Da):72,825
Last modified:November 1, 1995 - v1
Checksum:i1A724681E3BEA433
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57950.1
U00096 Genomic DNA Translation: AAC76181.1
AP009048 Genomic DNA Translation: BAE77193.1
PIRiG65104
RefSeqiNP_417616.1, NC_000913.3
WP_000249104.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76181; AAC76181; b3147
BAE77193; BAE77193; BAE77193
GeneIDi947663
KEGGiecj:JW3116
eco:b3147
PATRICifig|511145.12.peg.3242

Similar proteinsi

Entry informationi

Entry nameiLPOA_ECOLI
AccessioniPrimary (citable) accession number: P45464
Secondary accession number(s): Q2M963
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome