P45464 (LPOA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Penicillin-binding protein activator LpoA
Short name=PBP activator LpoA

Alternative name(s):
Lipoprotein activator of PBP from the outer membrane A

Gene names

Name:	lpoA
Synonyms:	yraM
Ordered Locus Names:	b3147, JW3116

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of peptidoglycan synthesis that is essential for the function of penicillin-binding protein 1A (PBP1a). Stimulates transpeptidase activity of PBP1a in vitro. Ref.3 Ref.4
Subunit structure	Interacts with PBP1a. Ref.3 Ref.4
Subcellular location	Cell outer membrane; Lipid-anchor; Periplasmic side. Note: Localizes at the sidewall of elongating cells. Ref.3 Ref.4
Disruption phenotype	Absence of both LpoA and LpoB leads to a decrease in peptide cross-linking and to cell lysis. Ref.4
Sequence similarities	Belongs to the LpoA family.

Ontologies

Keywords
Biological process	Cell shape Peptidoglycan synthesis
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from direct assay Ref.3. Source: UniProtKB regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	internal side of cell outer membrane Inferred from direct assay Ref.3. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enzyme binding Inferred from direct assay Ref.3. Source: EcoCyc enzyme regulator activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mrcA	P02918	4	EBI-557795,EBI-1126191

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 678	652	Penicillin-binding protein activator LpoA HAMAP MF_01890		PRO_0000169452
Amino acid modifications
	Lipidation	27	1	N-palmitoyl cysteine Potential
	Lipidation	27	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	P45464 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1A724681E3BEA433	FASTA	678	72,825
	10 20 30 40 50 60 MVPSTFSRLK AARCLPVVLA ALIFAGCGTH TPDQSTAYMQ GTAQADSAFY LQQMQQSSDD 70 80 90 100 110 120 TRINWQLLAI RALVKEGKTG QAVELFNQLP QELNDAQRRE KTLLAVEIKL AQKDFAGAQN 130 140 150 160 170 180 LLAKITPADL EQNQQARYWQ AKIDASQGRP SIDLLRALIA QEPLLGAKEK QQNIDATWQA 190 200 210 220 230 240 LSSMTQEQAN TLVINADENI LQGWLDLQRV WFDNRNDPDM MKAGIADWQK RYPNNPGAKM 250 260 270 280 290 300 LPTQLVNVKA FKPASTNKIA LLLPLNGQAA VFGRTIQQGF EAAKNIGTQP VAAQVAAAPA 310 320 330 340 350 360 ADVAEQPQPQ TVDGVASPAQ ASVSDLTGEQ PAAQPVPVSA PATSTAAVSA PANPSAELKI 370 380 390 400 410 420 YDTSSQPLSQ ILSQVQQDGA SIVVGPLLKN NVEELLKSNT PLNVLALNQP ENIENRVNIC 430 440 450 460 470 480 YFALSPEDEA RDAARHIRDQ GKQAPLVLIP RSSLGDRVAN AFAQEWQKLG GGTVLQQKFG 490 500 510 520 530 540 STSELRAGVN GGSGIALTGS PITLRATTDS GMTTNNPTLQ TTPTDDQFTN NGGRVDAVYI 550 560 570 580 590 600 VATPGEIAFI KPMIAMRNGS QSGATLYASS RSAQGTAGPD FRLEMEGLQY SEIPMLAGGN 610 620 630 640 650 660 LPLMQQALSA VNNDYSLARM YAMGVDAWSL ANHFSQMRQV QGFEINGNTG SLTANPDCVI 670 NRNLSWLQYQ QGQVVPVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Regulation of peptidoglycan synthesis by outer-membrane proteins." Typas A., Banzhaf M., van den Berg van Saparoea B., Verheul J., Biboy J., Nichols R.J., Zietek M., Beilharz K., Kannenberg K., von Rechenberg M., Breukink E., den Blaauwen T., Gross C.A., Vollmer W. Cell 143:1097-1109(2010) [PubMed: 21183073] [Abstract] Cited for: FUNCTION, INTERACTION WITH PBP1A, SUBCELLULAR LOCATION. Strain: K12.
[4]	"Lipoprotein cofactors located in the outer membrane activate bacterial cell wall polymerases." Paradis-Bleau C., Markovski M., Uehara T., Lupoli T.J., Walker S., Kahne D.E., Bernhardt T.G. Cell 143:1110-1120(2010) [PubMed: 21183074] [Abstract] Cited for: FUNCTION, INTERACTION WITH PBP1A, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA57950.1. U00096 Genomic DNA. Translation: AAC76181.1. AP009048 Genomic DNA. Translation: BAE77193.1.
PIR	G65104.
RefSeq	NP_417616.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P45464.
SMR	P45464. Positions 257-677.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12897N.
IntAct	P45464. 4 interactions.
MINT	MINT-1258091.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004967; EBESCP00000004967; EBESCG00000004055. EBESCT00000017292; EBESCP00000016583; EBESCG00000016349.
GeneID	947663.
GenomeReviews	Gene locus JW3116 in contig AP009048_GR. Gene locus b3147 in contig U00096_GR.
KEGG	ecj:JW3116. eco:b3147.
PATRIC	32121712. VBIEscCol129921_3242.
Organism-specific databases
EchoBASE	EB2631.
EcoGene	EG12778. lpoA.
Phylogenomic databases
eggNOG	COG3107.
GeneTree	EBGT00050000009777.
HOGENOM	HBG417064.
OMA	FCETPWL.
ProtClustDB	CLSK880607.
Enzyme and pathway databases
BioCyc	EcoCyc:G7642-MONOMER.
Gene expression databases
Genevestigator	P45464.
Family and domain databases
HAMAP	MF_01890. LpoA. [Tree]
InterPro	IPR007443. LpoA. [Graphical view]
KO	K07121.
Pfam	PF04348. LppC. 2 hits. [Graphical view]
PROSITE	PS51257. PROKAR_LIPOPROTEIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPOA_ECOLI

Accession

Primary (citable) accession number: P45464
Secondary accession number(s): Q2M963

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program