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Protein

Penicillin-binding protein activator LpoA

Gene

lpoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of peptidoglycan synthesis that is essential for the function of penicillin-binding protein 1A (PBP1a). Stimulates transpeptidase activity of PBP1a in vitro.2 Publications

GO - Molecular functioni

GO - Biological processi

  • peptidoglycan biosynthetic process Source: UniProtKB
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell shape, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7642-MONOMER.
ECOL316407:JW3116-MONOMER.
MetaCyc:G7642-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein activator LpoA
Short name:
PBP activator LpoA
Alternative name(s):
Lipoprotein activator of PBP from the outer membrane A
Gene namesi
Name:lpoA
Synonyms:yraM
Ordered Locus Names:b3147, JW3116
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12778. lpoA.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • periplasmic side of cell outer membrane Source: UniProtKB
  • periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Absence of both LpoA and LpoB leads to a decrease in peptide cross-linking and to cell lysis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000016945227 – 678Penicillin-binding protein activator LpoAAdd BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi27N-palmitoyl cysteineSequence analysis1
Lipidationi27S-diacylglycerol cysteineSequence analysis1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP45464.
PaxDbiP45464.
PRIDEiP45464.

Interactioni

Subunit structurei

Interacts with PBP1a.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mrcAP029184EBI-557795,EBI-1126191

Protein-protein interaction databases

BioGridi4261989. 55 interactors.
DIPiDIP-12897N.
IntActiP45464. 4 interactors.
MINTiMINT-1258091.
STRINGi511145.b3147.

Structurei

Secondary structure

1678
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 40Combined sources4
Helixi47 – 54Combined sources8
Helixi59 – 76Combined sources18
Helixi80 – 86Combined sources7
Helixi95 – 109Combined sources15
Turni110 – 113Combined sources4
Helixi115 – 122Combined sources8
Turni127 – 129Combined sources3
Helixi132 – 139Combined sources8
Helixi142 – 145Combined sources4
Helixi152 – 161Combined sources10
Turni162 – 164Combined sources3
Helixi167 – 181Combined sources15
Helixi186 – 189Combined sources4
Helixi200 – 214Combined sources15
Helixi218 – 231Combined sources14
Helixi236 – 238Combined sources3
Turni243 – 245Combined sources3
Helixi246 – 248Combined sources3
Beta strandi250 – 252Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MHKNMR-A28-256[»]
ProteinModelPortaliP45464.
SMRiP45464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpoA family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E1C. Bacteria.
COG3107. LUCA.
HOGENOMiHOG000125217.
InParanoidiP45464.
KOiK07121.
OMAiFCETPWL.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
HAMAPiMF_01890. LpoA. 1 hit.
InterProiIPR007443. LpoA.
IPR016026. Lytic_TGlyclase_suprhlx_U/L.
IPR028082. Peripla_BP_I.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF04348. LppC. 1 hit.
[Graphical view]
SUPFAMiSSF48435. SSF48435. 1 hit.
SSF53822. SSF53822. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPSTFSRLK AARCLPVVLA ALIFAGCGTH TPDQSTAYMQ GTAQADSAFY
60 70 80 90 100
LQQMQQSSDD TRINWQLLAI RALVKEGKTG QAVELFNQLP QELNDAQRRE
110 120 130 140 150
KTLLAVEIKL AQKDFAGAQN LLAKITPADL EQNQQARYWQ AKIDASQGRP
160 170 180 190 200
SIDLLRALIA QEPLLGAKEK QQNIDATWQA LSSMTQEQAN TLVINADENI
210 220 230 240 250
LQGWLDLQRV WFDNRNDPDM MKAGIADWQK RYPNNPGAKM LPTQLVNVKA
260 270 280 290 300
FKPASTNKIA LLLPLNGQAA VFGRTIQQGF EAAKNIGTQP VAAQVAAAPA
310 320 330 340 350
ADVAEQPQPQ TVDGVASPAQ ASVSDLTGEQ PAAQPVPVSA PATSTAAVSA
360 370 380 390 400
PANPSAELKI YDTSSQPLSQ ILSQVQQDGA SIVVGPLLKN NVEELLKSNT
410 420 430 440 450
PLNVLALNQP ENIENRVNIC YFALSPEDEA RDAARHIRDQ GKQAPLVLIP
460 470 480 490 500
RSSLGDRVAN AFAQEWQKLG GGTVLQQKFG STSELRAGVN GGSGIALTGS
510 520 530 540 550
PITLRATTDS GMTTNNPTLQ TTPTDDQFTN NGGRVDAVYI VATPGEIAFI
560 570 580 590 600
KPMIAMRNGS QSGATLYASS RSAQGTAGPD FRLEMEGLQY SEIPMLAGGN
610 620 630 640 650
LPLMQQALSA VNNDYSLARM YAMGVDAWSL ANHFSQMRQV QGFEINGNTG
660 670
SLTANPDCVI NRNLSWLQYQ QGQVVPVS
Length:678
Mass (Da):72,825
Last modified:November 1, 1995 - v1
Checksum:i1A724681E3BEA433
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57950.1.
U00096 Genomic DNA. Translation: AAC76181.1.
AP009048 Genomic DNA. Translation: BAE77193.1.
PIRiG65104.
RefSeqiNP_417616.1. NC_000913.3.
WP_000249104.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76181; AAC76181; b3147.
BAE77193; BAE77193; BAE77193.
GeneIDi947663.
KEGGiecj:JW3116.
eco:b3147.
PATRICi32121712. VBIEscCol129921_3242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57950.1.
U00096 Genomic DNA. Translation: AAC76181.1.
AP009048 Genomic DNA. Translation: BAE77193.1.
PIRiG65104.
RefSeqiNP_417616.1. NC_000913.3.
WP_000249104.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MHKNMR-A28-256[»]
ProteinModelPortaliP45464.
SMRiP45464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261989. 55 interactors.
DIPiDIP-12897N.
IntActiP45464. 4 interactors.
MINTiMINT-1258091.
STRINGi511145.b3147.

Proteomic databases

EPDiP45464.
PaxDbiP45464.
PRIDEiP45464.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76181; AAC76181; b3147.
BAE77193; BAE77193; BAE77193.
GeneIDi947663.
KEGGiecj:JW3116.
eco:b3147.
PATRICi32121712. VBIEscCol129921_3242.

Organism-specific databases

EchoBASEiEB2631.
EcoGeneiEG12778. lpoA.

Phylogenomic databases

eggNOGiENOG4105E1C. Bacteria.
COG3107. LUCA.
HOGENOMiHOG000125217.
InParanoidiP45464.
KOiK07121.
OMAiFCETPWL.

Enzyme and pathway databases

BioCyciEcoCyc:G7642-MONOMER.
ECOL316407:JW3116-MONOMER.
MetaCyc:G7642-MONOMER.

Miscellaneous databases

PROiP45464.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
HAMAPiMF_01890. LpoA. 1 hit.
InterProiIPR007443. LpoA.
IPR016026. Lytic_TGlyclase_suprhlx_U/L.
IPR028082. Peripla_BP_I.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF04348. LppC. 1 hit.
[Graphical view]
SUPFAMiSSF48435. SSF48435. 1 hit.
SSF53822. SSF53822. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiLPOA_ECOLI
AccessioniPrimary (citable) accession number: P45464
Secondary accession number(s): Q2M963
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.