ID   MASY_SOYBN              Reviewed;         564 AA.
AC   P45458;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   24-JAN-2024, entry version 100.
DE   RecName: Full=Malate synthase, glyoxysomal;
DE            Short=MS;
DE            EC=2.3.3.9;
DE   Flags: Fragment;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Maple Arrow; TISSUE=Cotyledon;
RX   PubMed=8547819; DOI=10.1007/bf00202659;
RA   Guex N., Henry H., Flach J., Richter H., Widmer F.;
RT   "Glyoxysomal malate dehydrogenase and malate synthase from soybean
RT   cotyledons (Glycine max L.): enzyme association, antibody production and
RT   cDNA cloning.";
RL   Planta 197:369-375(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR   EMBL; L01629; AAC37465.1; -; mRNA.
DR   PIR; T07690; T07690.
DR   AlphaFoldDB; P45458; -.
DR   SMR; P45458; -.
DR   STRING; 3847.P45458; -.
DR   PaxDb; 3847-GLYMA17G13730-1; -.
DR   eggNOG; KOG1261; Eukaryota.
DR   InParanoid; P45458; -.
DR   UniPathway; UPA00703; UER00720.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IBA:GO_Central.
DR   GO; GO:0006097; P:glyoxylate cycle; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR   Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR001465; Malate_synthase_TIM.
DR   InterPro; IPR048355; MS_C.
DR   InterPro; IPR048356; MS_N.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   NCBIfam; TIGR01344; malate_syn_A; 1.
DR   PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR   PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR   Pfam; PF20659; MS_C; 1.
DR   Pfam; PF20656; MS_N; 1.
DR   Pfam; PF01274; MS_TIM-barrel; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; Malate synthase G; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Glyoxylate bypass; Glyoxysome; Peroxisome; Reference proteome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           <1..564
FT                   /note="Malate synthase, glyoxysomal"
FT                   /id="PRO_0000166873"
FT   MOTIF           562..564
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        179
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        465
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   564 AA;  63909 MW;  EE175238AEA6185A CRC64;
     GTYGYPTPAV KKIESYYDVP EGVDIRGRYD AEFAKILTKD ALKFVADLQR EFRNHIKYAL
     ECRREAKKKY NEGALPEFDP ATTYIREQEW VCAPVPPAVA DRKVEITGPV DRKMVINALN
     SGAKVFMADF EDALSPGWEN LMRGQVNLKD AVAGTISLHD KARNRVYKLN DQTAKLFVRP
     RGWHLPEAHI LIDGEPATGC LVDFGLYFYH SYSAFRRTQG AGFGPFFYLP KMEHSREAKI
     WNNVFEKAEK VAGIERGSIR ATVLIETLPA VFQMNEILYE LKDHSVGLNC GRWDYIFSYV
     KTFQAHPDRL LPDRVLVGMT QHFMKSYSDL LIRTCHRRGV HAMGGMAAQI PIKEDPVANE
     VALELVRKDK LREVKAGHDG TWAAHPGLIP ACMEIFNNNM GNASNQIDTV KREDGANITE
     QDLLQIPRGA RTMEGLRLNT RVGIQYVAAW LTGSGSVPLY NLMEDAATAE ISRVQNWQWL
     KYGVELNGDG LGVKVNKELF GRVVEEEMAR IEKEVGTEKF KEGMYKEACK IFTRQCTSPM
     LDDFLTLDAY NYIVVHHPRE TSKL
//