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P45458 (MASY_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase, glyoxysomal

Short name=MS
EC=2.3.3.9
OrganismGlycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja

Protein attributes

Sequence length564 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 564›564Malate synthase, glyoxysomal
PRO_0000166873

Regions

Motif562 – 5643Microbody targeting signal Potential

Sites

Active site1791Proton acceptor By similarity
Active site4651Proton donor By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P45458 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: EE175238AEA6185A

FASTA56463,909
        10         20         30         40         50         60 
GTYGYPTPAV KKIESYYDVP EGVDIRGRYD AEFAKILTKD ALKFVADLQR EFRNHIKYAL 

        70         80         90        100        110        120 
ECRREAKKKY NEGALPEFDP ATTYIREQEW VCAPVPPAVA DRKVEITGPV DRKMVINALN 

       130        140        150        160        170        180 
SGAKVFMADF EDALSPGWEN LMRGQVNLKD AVAGTISLHD KARNRVYKLN DQTAKLFVRP 

       190        200        210        220        230        240 
RGWHLPEAHI LIDGEPATGC LVDFGLYFYH SYSAFRRTQG AGFGPFFYLP KMEHSREAKI 

       250        260        270        280        290        300 
WNNVFEKAEK VAGIERGSIR ATVLIETLPA VFQMNEILYE LKDHSVGLNC GRWDYIFSYV 

       310        320        330        340        350        360 
KTFQAHPDRL LPDRVLVGMT QHFMKSYSDL LIRTCHRRGV HAMGGMAAQI PIKEDPVANE 

       370        380        390        400        410        420 
VALELVRKDK LREVKAGHDG TWAAHPGLIP ACMEIFNNNM GNASNQIDTV KREDGANITE 

       430        440        450        460        470        480 
QDLLQIPRGA RTMEGLRLNT RVGIQYVAAW LTGSGSVPLY NLMEDAATAE ISRVQNWQWL 

       490        500        510        520        530        540 
KYGVELNGDG LGVKVNKELF GRVVEEEMAR IEKEVGTEKF KEGMYKEACK IFTRQCTSPM 

       550        560 
LDDFLTLDAY NYIVVHHPRE TSKL 

« Hide

References

[1]"Glyoxysomal malate dehydrogenase and malate synthase from soybean cotyledons (Glycine max L.): enzyme association, antibody production and cDNA cloning."
Guex N., Henry H., Flach J., Richter H., Widmer F.
Planta 197:369-375(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Maple Arrow.
Tissue: Cotyledon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L01629 mRNA. Translation: AAC37465.1.
PIRT07690.
UniGeneGma.31590.

3D structure databases

ProteinModelPortalP45458.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Gene expression databases

GenevestigatorP45458.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMASY_SOYBN
AccessionPrimary (citable) accession number: P45458
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways