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P45454

- DMA_SERMA

UniProt

P45454 - DMA_SERMA

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Protein
DNA adenine methylase
Gene
dam
Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionine By similarity
Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei54 – 541S-adenosyl-L-methionine By similarity
Binding sitei181 – 1811S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3001. M.SmaII.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.SmaII
Gene namesi
Name:dam
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270DNA adenine methylase
PRO_0000087997Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP45454.
SMRiP45454. Positions 3-270.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45454-1 [UniParc]FASTAAdd to Basket

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MKKNRAFLKW AGGKYPLVDE IRRHLPAGDC LIEPFVGAGS VFLNTDYDAY    50
ILADINSDLI NLYNIVKLRT DDFVRDARTL FADEFNNSDQ FYLLREEFNT 100
STEPYRRALL FLYLNRHCYN GLCRYNLRGE FNVPFGRYKK PYFPEEELYW 150
FAEKSRNATF VCEHYRDTMA KAAAGAVVYC DPPYAPLSAT ANFTAYHTNS 200
FSIADQQSLA HLAHQLSVES RVPVLISNHD TELTRDWYQH AALYVVKARR 250
TISRNILGRS KVNELLALYR 270
Length:270
Mass (Da):31,331
Last modified:November 1, 1995 - v1
Checksum:i8EEFFB163DF4288A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78412 Genomic DNA. Translation: CAA55177.1.
PIRiS47099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78412 Genomic DNA. Translation: CAA55177.1 .
PIRi S47099.

3D structure databases

ProteinModelPortali P45454.
SMRi P45454. Positions 3-270.
ModBasei Search...

Protein family/group databases

REBASEi 3001. M.SmaII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of a dam mutant of Serratia marcescens and nucleotide sequence of the dam region."
    Ostendorf T., Cherepanov P., de Vries J., Wackernagel W.
    J. Bacteriol. 181:3880-3885(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sr41.

Entry informationi

Entry nameiDMA_SERMA
AccessioniPrimary (citable) accession number: P45454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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