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P45454

- DMA_SERMA

UniProt

P45454 - DMA_SERMA

Protein

DNA adenine methylase

Gene

dam

Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    • Comment

    Functioni

    Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
    Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
    Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3001. M.SmaII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA adenine methylase (EC:2.1.1.72)
    Alternative name(s):
    DNA adenine methyltransferase
    Deoxyadenosyl-methyltransferase
    M.SmaII
    Gene namesi
    Name:dam
    OrganismiSerratia marcescens
    Taxonomic identifieri615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270DNA adenine methylasePRO_0000087997Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP45454.
    SMRiP45454. Positions 3-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45454-1 [UniParc]FASTAAdd to Basket

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    MKKNRAFLKW AGGKYPLVDE IRRHLPAGDC LIEPFVGAGS VFLNTDYDAY    50
    ILADINSDLI NLYNIVKLRT DDFVRDARTL FADEFNNSDQ FYLLREEFNT 100
    STEPYRRALL FLYLNRHCYN GLCRYNLRGE FNVPFGRYKK PYFPEEELYW 150
    FAEKSRNATF VCEHYRDTMA KAAAGAVVYC DPPYAPLSAT ANFTAYHTNS 200
    FSIADQQSLA HLAHQLSVES RVPVLISNHD TELTRDWYQH AALYVVKARR 250
    TISRNILGRS KVNELLALYR 270
    Length:270
    Mass (Da):31,331
    Last modified:November 1, 1995 - v1
    Checksum:i8EEFFB163DF4288A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78412 Genomic DNA. Translation: CAA55177.1.
    PIRiS47099.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78412 Genomic DNA. Translation: CAA55177.1 .
    PIRi S47099.

    3D structure databases

    ProteinModelPortali P45454.
    SMRi P45454. Positions 3-270.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3001. M.SmaII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a dam mutant of Serratia marcescens and nucleotide sequence of the dam region."
      Ostendorf T., Cherepanov P., de Vries J., Wackernagel W.
      J. Bacteriol. 181:3880-3885(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sr41.

    Entry informationi

    Entry nameiDMA_SERMA
    AccessioniPrimary (citable) accession number: P45454
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3