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P45454 (DMA_SERMA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA adenine methylase

EC=2.1.1.72
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.SmaII
Gene names
Name:dam
OrganismSerratia marcescens
Taxonomic identifier615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
   Biological processDNA replication
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

site-specific DNA-methyltransferase (adenine-specific) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270DNA adenine methylase
PRO_0000087997

Sites

Binding site101S-adenosyl-L-methionine By similarity
Binding site141S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site541S-adenosyl-L-methionine By similarity
Binding site1811S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
P45454 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 8EEFFB163DF4288A

FASTA27031,331
        10         20         30         40         50         60 
MKKNRAFLKW AGGKYPLVDE IRRHLPAGDC LIEPFVGAGS VFLNTDYDAY ILADINSDLI 

        70         80         90        100        110        120 
NLYNIVKLRT DDFVRDARTL FADEFNNSDQ FYLLREEFNT STEPYRRALL FLYLNRHCYN 

       130        140        150        160        170        180 
GLCRYNLRGE FNVPFGRYKK PYFPEEELYW FAEKSRNATF VCEHYRDTMA KAAAGAVVYC 

       190        200        210        220        230        240 
DPPYAPLSAT ANFTAYHTNS FSIADQQSLA HLAHQLSVES RVPVLISNHD TELTRDWYQH 

       250        260        270 
AALYVVKARR TISRNILGRS KVNELLALYR 

« Hide

References

[1]"Characterization of a dam mutant of Serratia marcescens and nucleotide sequence of the dam region."
Ostendorf T., Cherepanov P., de Vries J., Wackernagel W.
J. Bacteriol. 181:3880-3885(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sr41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78412 Genomic DNA. Translation: CAA55177.1.
PIRS47099.

3D structure databases

ProteinModelPortalP45454.
SMRP45454. Positions 3-270.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3001. M.SmaII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDMA_SERMA
AccessionPrimary (citable) accession number: P45454
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries