P45454 (DMA_SERMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA adenine methylase
EC=2.1.1.72

Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.SmaII

Gene names

Name:

dam

Organism

Serratia marcescens

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	DNA adenine methylase		PRO_0000087997
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	14	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	54	1	S-adenosyl-L-methionine By similarity
	Binding site	181	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45454 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 8EEFFB163DF4288A	FASTA	270	31,331
	10 20 30 40 50 60 MKKNRAFLKW AGGKYPLVDE IRRHLPAGDC LIEPFVGAGS VFLNTDYDAY ILADINSDLI 70 80 90 100 110 120 NLYNIVKLRT DDFVRDARTL FADEFNNSDQ FYLLREEFNT STEPYRRALL FLYLNRHCYN 130 140 150 160 170 180 GLCRYNLRGE FNVPFGRYKK PYFPEEELYW FAEKSRNATF VCEHYRDTMA KAAAGAVVYC 190 200 210 220 230 240 DPPYAPLSAT ANFTAYHTNS FSIADQQSLA HLAHQLSVES RVPVLISNHD TELTRDWYQH 250 260 270 AALYVVKARR TISRNILGRS KVNELLALYR

References

[1]	"Characterization of a dam mutant of Serratia marcescens and nucleotide sequence of the dam region." Ostendorf T., Cherepanov P., de Vries J., Wackernagel W. J. Bacteriol. 181:3880-3885(1999) [PubMed: 10383952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sr41.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78412 Genomic DNA. Translation: CAA55177.1.
PIR	S47099.
3D structure databases
ProteinModelPortal	P45454.
SMR	P45454. Positions 3-270.
ModBase	Search...
Protein family/group databases
REBASE	3001. M.SmaII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR023095. Ade_MeTrfase_dom_2. IPR002052. DNA_methylase_N6_adenine_CS. IPR012263. M_m6A_EcoRV. IPR012327. MeTrfase_D12. [Graphical view]
Gene3D	G3DSA:1.10.1020.10. Ade_MeTrfase_dom_2. 1 hit.
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PIRSF	PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. Dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMA_SERMA

Accession

Primary (citable) accession number: P45454

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 31, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program