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P45448 (NR5A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear receptor subfamily 5 group A member 2
Alternative name(s):
Liver receptor homolog 1
Short name=LRH-1
Gene names
Name:Nr5a2
Synonyms:Lrh1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to promoters containing the sequence element 5'-AACGACCGACCTTGAG-3'. Plays a role in the regulation of gene expression in liver and pancreas. May play a role in embryonic development By similarity.

Subunit structure

Binds DNA as a monomer By similarity. Interacts with GRIP1, NCOA2 and NR0B2. Ref.3

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR5 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainZinc-finger
   LigandDNA-binding
Lipid-binding
Metal-binding
Zinc
   Molecular functionReceptor
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbile acid metabolic process

Inferred from mutant phenotype PubMed 15014077. Source: MGI

cholesterol homeostasis

Inferred from mutant phenotype PubMed 15014077. Source: MGI

endocrine pancreas development

Traceable author statement. Source: Reactome

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 20937355. Source: BHF-UCL

positive regulation of viral genome replication

Inferred from electronic annotation. Source: Ensembl

regulation of cell proliferation

Inferred from genetic interaction PubMed 15684064. Source: MGI

regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 19796622. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 15014077PubMed 15798202PubMed 16912278. Source: MGI

phospholipid binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Nuclear receptor subfamily 5 group A member 2
PRO_0000053736

Regions

DNA binding104 – 17572Nuclear receptor
Zinc finger107 – 12721NR C4-type
Zinc finger143 – 16725NR C4-type
Region361 – 40949Ligand-binding By similarity
Motif176 – 20530FTZ-F1 box

Sites

Binding site5351Lipid headgroup By similarity
Binding site5391Lipid headgroup By similarity

Secondary structure

............................... 560
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P45448 [UniParc].

Last modified May 30, 2000. Version 3.
Checksum: 186F8C4025934643

FASTA56064,020
        10         20         30         40         50         60 
MSASLDTGDF QEFLKHGLTA IASAPGSETR HSPKREEQLR EKRAGLPDRH RRPIPARSRL 

        70         80         90        100        110        120 
VMLPKVETEA PGLVRSHGEQ GQMPENMQVS QFKMVNYSYD EDLEELCPVC GDKVSGYHYG 

       130        140        150        160        170        180 
LLTCESCKGF FKRTVQNQKR YTCIENQNCQ IDKTQRKRCP YCRFKKCIDV GMKLEAVRAD 

       190        200        210        220        230        240 
RMRGGRNKFG PMYKRDRALK QQKKALIRAN GLKLEAMSQV IQAMPSDLTS AIQNIHSASK 

       250        260        270        280        290        300 
GLPLSHVALP PTDYDRSPFV TSPISMTMPP HSSLHGYQPY GHFPSRAIKS EYPDPYSSSP 

       310        320        330        340        350        360 
ESMMGYSYMD GYQTNSPASI PHLILELLKC EPDEPQVQAK IMAYLQQEQS NRNRQEKLSA 

       370        380        390        400        410        420 
FGLLCKMADQ TLFSIVEWAR SSIFFRELKV DDQMKLLQNC WSELLILDHI YRQVAHGKEG 

       430        440        450        460        470        480 
TIFLVTGEHV DYSTIISHTE VAFNNLLSLA QELVVRLRSL QFDQREFVCL KFLVLFSSDV 

       490        500        510        520        530        540 
KNLENLQLVE GVQEQVNAAL LDYTVCNYPQ QTEKFGQLLL RLPEIRAISK QAEDYLYYKH 

       550        560 
VNGDVPYNNL LIEMLHAKRA 

« Hide

References

« Hide 'large scale' references
[1]"LRH-1: a nuclear hormone receptor active in the absence of exogenous ligands."
Tugwood J.D., Issemann I., Green S.
Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Structural basis for ligand-independent activation of the orphan nuclear receptor LRH-1."
Sablin E.P., Krylova I.N., Fletterick R.J., Ingraham H.A.
Mol. Cell 11:1575-1585(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 313-560, INTERACTION WITH NR0B2 AND GRIP1.
[4]"Structural and biochemical basis for selective repression of the orphan nuclear receptor liver receptor homolog 1 by small heterodimer partner."
Li Y., Choi M., Suino K., Kovach A., Daugherty J., Kliewer S.A., Xu H.E.
Proc. Natl. Acad. Sci. U.S.A. 102:9505-9510(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 318-560 IN COMPLEX WITH NR0B2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M81385 mRNA. Translation: AAA39447.1.
BC137845 mRNA. Translation: AAI37846.1.
CCDSCCDS15328.1.
PIRS27874.
RefSeqNP_109601.1. NM_030676.3.
UniGeneMm.16794.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PK5X-ray2.40A/B313-560[»]
1ZH7X-ray2.50A/B318-560[»]
3F5CX-ray3.00A313-560[»]
ProteinModelPortalP45448.
SMRP45448. Positions 105-560.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204976. 3 interactions.

PTM databases

PhosphoSiteP45448.

Proteomic databases

PaxDbP45448.
PRIDEP45448.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027649; ENSMUSP00000027649; ENSMUSG00000026398.
GeneID26424.
KEGGmmu:26424.
UCSCuc007cva.2. mouse.

Organism-specific databases

CTD2494.
MGIMGI:1346834. Nr5a2.

Phylogenomic databases

eggNOGNOG240365.
GeneTreeENSGT00750000117458.
HOGENOMHOG000063718.
HOVERGENHBG106677.
InParanoidP45448.
KOK08027.
OMANTFGLMC.
OrthoDBEOG7BS4B9.
PhylomeDBP45448.
TreeFamTF350737.

Enzyme and pathway databases

ReactomeREACT_13641. Regulation of Beta-Cell Development.
REACT_188576. Developmental Biology.

Gene expression databases

ArrayExpressP45448.
BgeeP45448.
CleanExMM_NR5A2.
GenevestigatorP45448.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR016355. Steroidogenic_factor_1.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
PRINTSPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 2 hits.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP45448.
NextBio304453.
PROP45448.
SOURCESearch...

Entry information

Entry nameNR5A2_MOUSE
AccessionPrimary (citable) accession number: P45448
Secondary accession number(s): B9EHF9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot