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Protein

Nuclear receptor subfamily 5 group A member 2

Gene

Nr5a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei535Lipid headgroupBy similarity1
Binding sitei539Lipid headgroupBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi104 – 175Nuclear receptorPROSITE-ProRule annotationAdd BLAST72
Zinc fingeri107 – 127NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri143 – 167NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • bile acid metabolic process Source: MGI
  • calcineurin-mediated signaling Source: Ensembl
  • cellular response to leukemia inhibitory factor Source: MGI
  • cholesterol homeostasis Source: MGI
  • endocrine pancreas development Source: Reactome
  • epithelial cell differentiation Source: MGI
  • hormone-mediated signaling pathway Source: GO_Central
  • pancreas morphogenesis Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • positive regulation of viral genome replication Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB

Keywordsi

Molecular functionDNA-binding, Receptor
Biological processTranscription, Transcription regulation
LigandLipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-383280 Nuclear Receptor transcription pathway
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 5 group A member 2
Alternative name(s):
Liver receptor homolog 1
Short name:
LRH-1
Gene namesi
Name:Nr5a2
Synonyms:Lrh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1346834 Nr5a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537361 – 560Nuclear receptor subfamily 5 group A member 2Add BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki289Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Post-translational modificationi

Sumoylated by SUMO1 at Lys-289 during the hepatic acute phase response, leading to promote interaction with GPS2 and prevent N-Cor corepressor complex dissociation.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP45448
PaxDbiP45448
PRIDEiP45448

PTM databases

iPTMnetiP45448
PhosphoSitePlusiP45448

Expressioni

Gene expression databases

BgeeiENSMUSG00000026398
CleanExiMM_NR5A2
ExpressionAtlasiP45448 baseline and differential
GenevisibleiP45448 MM

Interactioni

Subunit structurei

Binds DNA as a monomer (By similarity). Interacts with GRIP1, NCOA2 and NR0B2 (PubMed:12820970, PubMed:15976031). Interacts (when sumoylated) with GPS2; interaction with GPS2 onto hepatic acute phase protein promoters prevents N-Cor corepressor complex dissociation (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi204976, 3 interactors
STRINGi10090.ENSMUSP00000027649

Structurei

Secondary structure

1560
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi322 – 329Combined sources8
Helixi334 – 351Combined sources18
Beta strandi354 – 356Combined sources3
Helixi361 – 381Combined sources21
Helixi385 – 387Combined sources3
Helixi390 – 416Combined sources27
Beta strandi419 – 423Combined sources5
Beta strandi429 – 431Combined sources3
Helixi432 – 437Combined sources6
Helixi441 – 459Combined sources19
Helixi464 – 475Combined sources12
Helixi486 – 507Combined sources22
Helixi514 – 519Combined sources6
Helixi522 – 541Combined sources20
Helixi549 – 555Combined sources7
Turni556 – 558Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PK5X-ray2.40A/B313-560[»]
1ZH7X-ray2.50A/B318-560[»]
3F5CX-ray3.00A313-560[»]
ProteinModelPortaliP45448
SMRiP45448
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45448

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini319 – 558NR LBDPROSITE-ProRule annotationAdd BLAST240

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 205FTZ-F1 boxAdd BLAST30

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri107 – 127NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri143 – 167NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4218 Eukaryota
ENOG410YWC2 LUCA
GeneTreeiENSGT00870000136388
HOGENOMiHOG000063718
HOVERGENiHBG106677
InParanoidiP45448
KOiK08027
OMAiTQVIQAM
OrthoDBiEOG091G06RK
PhylomeDBiP45448
TreeFamiTF350737

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR035500 NHR_like_dom_sf
IPR016355 NR5_fam
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PANTHERiPTHR24086 PTHR24086, 1 hit
PfamiView protein in Pfam
PF00104 Hormone_recep, 1 hit
PF00105 zf-C4, 1 hit
PIRSFiPIRSF002530 Nuc_orph_FTZ-F1, 1 hit
PRINTSiPR00398 STRDHORMONER
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 2 hits
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P45448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASLDTGDF QEFLKHGLTA IASAPGSETR HSPKREEQLR EKRAGLPDRH
60 70 80 90 100
RRPIPARSRL VMLPKVETEA PGLVRSHGEQ GQMPENMQVS QFKMVNYSYD
110 120 130 140 150
EDLEELCPVC GDKVSGYHYG LLTCESCKGF FKRTVQNQKR YTCIENQNCQ
160 170 180 190 200
IDKTQRKRCP YCRFKKCIDV GMKLEAVRAD RMRGGRNKFG PMYKRDRALK
210 220 230 240 250
QQKKALIRAN GLKLEAMSQV IQAMPSDLTS AIQNIHSASK GLPLSHVALP
260 270 280 290 300
PTDYDRSPFV TSPISMTMPP HSSLHGYQPY GHFPSRAIKS EYPDPYSSSP
310 320 330 340 350
ESMMGYSYMD GYQTNSPASI PHLILELLKC EPDEPQVQAK IMAYLQQEQS
360 370 380 390 400
NRNRQEKLSA FGLLCKMADQ TLFSIVEWAR SSIFFRELKV DDQMKLLQNC
410 420 430 440 450
WSELLILDHI YRQVAHGKEG TIFLVTGEHV DYSTIISHTE VAFNNLLSLA
460 470 480 490 500
QELVVRLRSL QFDQREFVCL KFLVLFSSDV KNLENLQLVE GVQEQVNAAL
510 520 530 540 550
LDYTVCNYPQ QTEKFGQLLL RLPEIRAISK QAEDYLYYKH VNGDVPYNNL
560
LIEMLHAKRA
Length:560
Mass (Da):64,020
Last modified:May 30, 2000 - v3
Checksum:i186F8C4025934643
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81385 mRNA Translation: AAA39447.1
BC137845 mRNA Translation: AAI37846.1
CCDSiCCDS15328.1
PIRiS27874
RefSeqiNP_109601.1, NM_030676.3
UniGeneiMm.16794

Genome annotation databases

EnsembliENSMUST00000027649; ENSMUSP00000027649; ENSMUSG00000026398
GeneIDi26424
KEGGimmu:26424
UCSCiuc007cva.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNR5A2_MOUSE
AccessioniPrimary (citable) accession number: P45448
Secondary accession number(s): B9EHF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health