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P45448

- NR5A2_MOUSE

UniProt

P45448 - NR5A2_MOUSE

Protein

Nuclear receptor subfamily 5 group A member 2

Gene

Nr5a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 3 (30 May 2000)
      Previous versions | rss
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    Functioni

    Binds to promoters containing the sequence element 5'-AACGACCGACCTTGAG-3'. Plays a role in the regulation of gene expression in liver and pancreas. May play a role in embryonic development By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei535 – 5351Lipid headgroupBy similarity
    Binding sitei539 – 5391Lipid headgroupBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi104 – 17572Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri107 – 12721NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri143 – 16725NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. phospholipid binding Source: Ensembl
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    5. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    6. steroid hormone receptor activity Source: InterPro
    7. zinc ion binding Source: InterPro

    GO - Biological processi

    1. bile acid metabolic process Source: MGI
    2. cholesterol homeostasis Source: MGI
    3. endocrine pancreas development Source: Reactome
    4. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    5. positive regulation of viral genome replication Source: Ensembl
    6. regulation of cell proliferation Source: MGI
    7. regulation of transcription, DNA-templated Source: UniProtKB

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Lipid-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_208465. Regulation of gene expression in early pancreatic precursor cells.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 5 group A member 2
    Alternative name(s):
    Liver receptor homolog 1
    Short name:
    LRH-1
    Gene namesi
    Name:Nr5a2
    Synonyms:Lrh1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1346834. Nr5a2.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560Nuclear receptor subfamily 5 group A member 2PRO_0000053736Add
    BLAST

    Proteomic databases

    PaxDbiP45448.
    PRIDEiP45448.

    PTM databases

    PhosphoSiteiP45448.

    Expressioni

    Gene expression databases

    ArrayExpressiP45448.
    BgeeiP45448.
    CleanExiMM_NR5A2.
    GenevestigatoriP45448.

    Interactioni

    Subunit structurei

    Binds DNA as a monomer By similarity. Interacts with GRIP1, NCOA2 and NR0B2.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi204976. 3 interactions.

    Structurei

    Secondary structure

    1
    560
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi322 – 3298
    Helixi334 – 35118
    Beta strandi354 – 3563
    Helixi361 – 38121
    Helixi385 – 3873
    Helixi390 – 41627
    Beta strandi419 – 4235
    Beta strandi429 – 4313
    Helixi432 – 4376
    Helixi441 – 45919
    Helixi464 – 47512
    Helixi486 – 50722
    Helixi514 – 5196
    Helixi522 – 54120
    Helixi549 – 5557
    Turni556 – 5583

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PK5X-ray2.40A/B313-560[»]
    1ZH7X-ray2.50A/B318-560[»]
    3F5CX-ray3.00A313-560[»]
    ProteinModelPortaliP45448.
    SMRiP45448. Positions 105-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP45448.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni361 – 40949Ligand-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi176 – 20530FTZ-F1 boxAdd
    BLAST

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri107 – 12721NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri143 – 16725NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG240365.
    GeneTreeiENSGT00750000117458.
    HOGENOMiHOG000063718.
    HOVERGENiHBG106677.
    InParanoidiP45448.
    KOiK08027.
    OMAiNTFGLMC.
    OrthoDBiEOG7BS4B9.
    PhylomeDBiP45448.
    TreeFamiTF350737.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR016355. Steroidogenic_factor_1.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
    PRINTSiPR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 2 hits.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45448-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSASLDTGDF QEFLKHGLTA IASAPGSETR HSPKREEQLR EKRAGLPDRH    50
    RRPIPARSRL VMLPKVETEA PGLVRSHGEQ GQMPENMQVS QFKMVNYSYD 100
    EDLEELCPVC GDKVSGYHYG LLTCESCKGF FKRTVQNQKR YTCIENQNCQ 150
    IDKTQRKRCP YCRFKKCIDV GMKLEAVRAD RMRGGRNKFG PMYKRDRALK 200
    QQKKALIRAN GLKLEAMSQV IQAMPSDLTS AIQNIHSASK GLPLSHVALP 250
    PTDYDRSPFV TSPISMTMPP HSSLHGYQPY GHFPSRAIKS EYPDPYSSSP 300
    ESMMGYSYMD GYQTNSPASI PHLILELLKC EPDEPQVQAK IMAYLQQEQS 350
    NRNRQEKLSA FGLLCKMADQ TLFSIVEWAR SSIFFRELKV DDQMKLLQNC 400
    WSELLILDHI YRQVAHGKEG TIFLVTGEHV DYSTIISHTE VAFNNLLSLA 450
    QELVVRLRSL QFDQREFVCL KFLVLFSSDV KNLENLQLVE GVQEQVNAAL 500
    LDYTVCNYPQ QTEKFGQLLL RLPEIRAISK QAEDYLYYKH VNGDVPYNNL 550
    LIEMLHAKRA 560
    Length:560
    Mass (Da):64,020
    Last modified:May 30, 2000 - v3
    Checksum:i186F8C4025934643
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M81385 mRNA. Translation: AAA39447.1.
    BC137845 mRNA. Translation: AAI37846.1.
    CCDSiCCDS15328.1.
    PIRiS27874.
    RefSeqiNP_109601.1. NM_030676.3.
    UniGeneiMm.16794.

    Genome annotation databases

    EnsembliENSMUST00000027649; ENSMUSP00000027649; ENSMUSG00000026398.
    GeneIDi26424.
    KEGGimmu:26424.
    UCSCiuc007cva.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M81385 mRNA. Translation: AAA39447.1 .
    BC137845 mRNA. Translation: AAI37846.1 .
    CCDSi CCDS15328.1.
    PIRi S27874.
    RefSeqi NP_109601.1. NM_030676.3.
    UniGenei Mm.16794.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PK5 X-ray 2.40 A/B 313-560 [» ]
    1ZH7 X-ray 2.50 A/B 318-560 [» ]
    3F5C X-ray 3.00 A 313-560 [» ]
    ProteinModelPortali P45448.
    SMRi P45448. Positions 105-560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204976. 3 interactions.

    PTM databases

    PhosphoSitei P45448.

    Proteomic databases

    PaxDbi P45448.
    PRIDEi P45448.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027649 ; ENSMUSP00000027649 ; ENSMUSG00000026398 .
    GeneIDi 26424.
    KEGGi mmu:26424.
    UCSCi uc007cva.2. mouse.

    Organism-specific databases

    CTDi 2494.
    MGIi MGI:1346834. Nr5a2.

    Phylogenomic databases

    eggNOGi NOG240365.
    GeneTreei ENSGT00750000117458.
    HOGENOMi HOG000063718.
    HOVERGENi HBG106677.
    InParanoidi P45448.
    KOi K08027.
    OMAi NTFGLMC.
    OrthoDBi EOG7BS4B9.
    PhylomeDBi P45448.
    TreeFami TF350737.

    Enzyme and pathway databases

    Reactomei REACT_208465. Regulation of gene expression in early pancreatic precursor cells.

    Miscellaneous databases

    EvolutionaryTracei P45448.
    NextBioi 304453.
    PROi P45448.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P45448.
    Bgeei P45448.
    CleanExi MM_NR5A2.
    Genevestigatori P45448.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR016355. Steroidogenic_factor_1.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
    PRINTSi PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 2 hits.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "LRH-1: a nuclear hormone receptor active in the absence of exogenous ligands."
      Tugwood J.D., Issemann I., Green S.
      Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Structural basis for ligand-independent activation of the orphan nuclear receptor LRH-1."
      Sablin E.P., Krylova I.N., Fletterick R.J., Ingraham H.A.
      Mol. Cell 11:1575-1585(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 313-560, INTERACTION WITH NR0B2 AND GRIP1.
    4. "Structural and biochemical basis for selective repression of the orphan nuclear receptor liver receptor homolog 1 by small heterodimer partner."
      Li Y., Choi M., Suino K., Kovach A., Daugherty J., Kliewer S.A., Xu H.E.
      Proc. Natl. Acad. Sci. U.S.A. 102:9505-9510(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 318-560 IN COMPLEX WITH NR0B2.

    Entry informationi

    Entry nameiNR5A2_MOUSE
    AccessioniPrimary (citable) accession number: P45448
    Secondary accession number(s): B9EHF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 131 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3