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P45404 (CCMF_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome c-type biogenesis protein CycK
Gene names
Name:cycK
Ordered Locus Names:R01019
ORF Names:SMc02363
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium ›

Protein attributes

Sequence length676 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family.

Ontologies

Keywords
   Biological processCytochrome c-type biogenesis
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcytochrome complex assembly

Inferred from electronic annotation. Source: UniProtKB-KW

respiratory chain complex IV assembly

Inferred from electronic annotation. Source: InterPro

   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionheme transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 676676Cytochrome c-type biogenesis protein CycK
PRO_0000201587

Regions

Transmembrane8 – 2821Helical; Potential
Transmembrane42 – 6221Helical; Potential
Transmembrane94 – 11421Helical; Potential
Transmembrane123 – 14321Helical; Potential
Transmembrane175 – 19521Helical; Potential
Transmembrane210 – 23021Helical; Potential
Transmembrane233 – 25321Helical; Potential
Transmembrane273 – 29321Helical; Potential
Transmembrane311 – 33121Helical; Potential
Transmembrane356 – 37621Helical; Potential
Transmembrane393 – 41321Helical; Potential
Transmembrane418 – 43821Helical; Potential
Transmembrane445 – 46521Helical; Potential
Transmembrane493 – 51321Helical; Potential
Transmembrane617 – 63721Helical; Potential

Experimental info

Sequence conflict3191V → I in CAA57906. Ref.1
Sequence conflict6581H → R in CAA57906. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P45404 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: A2DD652280895E0D

FASTA67672,611
        10         20         30         40         50         60 
MIIELGHYAL VLALATAIIQ GVLPVLGVRR GDPSLMGLAA NAALVCFLLV AFSFAVLTFA 

        70         80         90        100        110        120 
YVTSDFSVKN VWENSHSLKP LIYKITGVWG NHEGSMLLWL LILVFFSAMV ALFGRNLPET 

       130        140        150        160        170        180 
LKANVLAVQA WIATAFAFFV LLTSNPFARL VPAPGEGRDL NPVLQDIGLA IHPPLLYLGY 

       190        200        210        220        230        240 
VGFSVCFSFA VAALIEGRID AAWARWVRPW TLAAWTFLTA GIAMGSYWAY YELGWGGWWF 

       250        260        270        280        290        300 
WDPVENASFM PWLAGTALLH SALVMEKREA LKIWTVLLAI MTFSLSLLGT FLVRSGVLTS 

       310        320        330        340        350        360 
VHAFATDPTR GVFILAILVV FIGGAFSLFA FRASHLKAGG IFAPVSREGA LVVNNLILTT 

       370        380        390        400        410        420 
ATATVLTGTL YPLVLEALTG DKISVGAPFF NMTFGLLMLP LIAVVPFGPL LAWKRGDLAG 

       430        440        450        460        470        480 
AAQRLFAAAA LGLLAAAICY YAVNGGPVLA PFGLGLGVYL ILGALTDLVL RSGLGKVAAG 

       490        500        510        520        530        540 
VAWRRLSGLP RSAFGTALAH IGLGITLIGI VTVTAFETET VVEMKPGAVV DVGRYSLRFD 

       550        560        570        580        590        600 
GMREGRGPNY TEDAGHFTVS RGGVEVTEIW SSKRLYSARR MPTTEAGIRT FGLSQLYVSL 

       610        620        630        640        650        660 
GDDMADGGIV VRIWWKPLIL CIWGGALVMM AGGVVSLSDR RLRVGAPARA RKALALDHDD 

       670 
FRSDRPKIIN VIDSKG

« Hide

References

« Hide 'large scale' references
[1]"The cycHJKL genes of Rhizobium meliloti involved in cytochrome c biogenesis are required for 'respiratory' nitrate reduction ex planta and for nitrogen fixation during symbiosis."
Kereszt A., Slaska-Kiss K., Putnoky P., Banfalvi Z., Kondorosi A.
Mol. Gen. Genet. 247:39-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AK631.
[2]"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021."
Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. expand/collapse author list , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.
Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X82560 Genomic DNA. Translation: CAA57906.1.
AL591688 Genomic DNA. Translation: CAC45591.1.
PIRS54750.
RefSeqNP_385125.1. NC_003047.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING266834.SMc02363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC45591; CAC45591; SMc02363.
GeneID1232662.
KEGGsme:SMc02363.
PATRIC23631323. VBISinMel96828_2423.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1138.
HOGENOMHOG000274659.
KOK02198.
OMACLTYAFM.
ProtClustDBCLSK881482.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-1042-MONOMER.

Family and domain databases

InterProIPR002541. Cyt_c_assembly.
IPR003567. Cyt_c_biogenesis.
IPR003568. Cyt_c_biogenesis_CcmF.
[Graphical view]
PfamPF01578. Cytochrom_C_asm. 1 hit.
[Graphical view]
PRINTSPR01410. CCBIOGENESIS.
PR01411. CCMFBIOGNSIS.
TIGRFAMsTIGR00353. nrfE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCCMF_RHIME
AccessionPrimary (citable) accession number: P45404
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2000
Last modified: May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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