P45403 (CCMF_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c-type biogenesis protein CycK

Gene names

Name:	cycK
Ordered Locus Names:	blr3127

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium japonicum

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain complex IV assembly Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Cytochrome c-type biogenesis protein CycK		PRO_0000201585
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	42 – 62	21	Helical; Potential
	Transmembrane	94 – 114	21	Helical; Potential
	Transmembrane	123 – 143	21	Helical; Potential
	Transmembrane	175 – 195	21	Helical; Potential
	Transmembrane	210 – 230	21	Helical; Potential
	Transmembrane	233 – 253	21	Helical; Potential
	Transmembrane	273 – 293	21	Helical; Potential
	Transmembrane	311 – 331	21	Helical; Potential
	Transmembrane	350 – 370	21	Helical; Potential
	Transmembrane	392 – 412	21	Helical; Potential
	Transmembrane	419 – 439	21	Helical; Potential
	Transmembrane	445 – 465	21	Helical; Potential
	Transmembrane	492 – 512	21	Helical; Potential
	Transmembrane	617 – 637	21	Helical; Potential
Experimental info
	Sequence conflict	205	1	R → L in CAA80244. Ref.1
	Sequence conflict	205	1	R → L in CAA86575. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P45403 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: 15B685136CA6D741	FASTA	660	71,127
	10 20 30 40 50 60 MIAESGHYAL VLALGLALIQ SIVPLIGARL RDDALMSVAR STALAQLLFV GASFVALVML 70 80 90 100 110 120 HVNSDFSVAN VYENSHSMKP LLYKITGVWG NHEGSMLLWV SILALFGGLV AAFGNNLPLS 130 140 150 160 170 180 LRAHVLAVQA WIASAFYLFI LVTSNPFLRI ANPPIEGRDL NPVLQDIGLA VHPPMLYLGY 190 200 210 220 230 240 VGFSISFSFA IAALMEGRID AAWARWVRPW TLVAWIFLTL GIAMGSYWAY YELGWGGWWF 250 260 270 280 290 300 WDPVENASLM PWLAGTALLH SALVMEKRNA LKVWTILLSI LTFSLSLLGT FLVRSGVITS 310 320 330 340 350 360 VHAFATDPTR GVFILLILCL FIGGSLSLFA GRATSLKQGG LFAPISREGA LVLNNLLLTV 370 380 390 400 410 420 ACAVVLFGTL YPLAMEMLAD FKMSVGAPFY NLTFAPLFAL LLLAVPFGPM LAWKRGDLLG 430 440 450 460 470 480 VTQRLLAAGV AGLVVVAIVW AWTRGGSALA PLAIGLGVFV IAGAVTDLVE RTGLVRLPLA 490 500 510 520 530 540 TALQRARGLP RSAWGAALAH AGLGVALIGI VCETTWNSEY IATMKRSDVA HVAGYDVKLD 550 560 570 580 590 600 GLFQRQGPNY REMIAEFNVS RDGETLSVMT PSKRSFTTRG SSTTEAALLT RGASQLYISL 610 620 630 640 650 660 GDANAEGAIA VRIYHKPLVL LIWWGPVLMA FGGVLSLSDR RLRVGAPKPA RAKQRLQPAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cycHJKL gene cluster plays an essential role in the biogenesis of c-type cytochromes in Bradyrhizobium japonicum." Ritz D., Thoeny-Meyer L., Hennecke H. Mol. Gen. Genet. 247:27-38(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z22517 Genomic DNA. Translation: CAA80244.1. Z46607 Genomic DNA. Translation: CAA86575.1. BA000040 Genomic DNA. Translation: BAC48392.1.
PIR	S54746.
RefSeq	NP_769767.1. NC_004463.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	224911.blr3127.
Protein family/group databases
TCDB	9.B.14.1.2. putative heme handling protein (HHP) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC48392; BAC48392; BAC48392.
GeneID	1053038.
KEGG	bja:blr3127.
PATRIC	21189718. VBIBraJap65052_3117.
Phylogenomic databases
eggNOG	COG1138.
HOGENOM	HOG000274659.
KO	K02198.
OMA	HRSETIT.
ProtClustDB	CLSK910967.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-3151-MONOMER.
Family and domain databases
InterPro	IPR002541. Cyt_c_assembly. IPR003567. Cyt_c_biogenesis. IPR003568. Cyt_c_biogenesis_CcmF. [Graphical view]
Pfam	PF01578. Cytochrom_C_asm. 1 hit. [Graphical view]
PRINTS	PR01410. CCBIOGENESIS. PR01411. CCMFBIOGNSIS.
TIGRFAMs	TIGR00353. nrfE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCMF_BRAJA

Accession

Primary (citable) accession number: P45403

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	February 28, 2003
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program