P45394 (YRBG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inner membrane protein YrbG

Gene names

Name:	yrbG
Ordered Locus Names:	b3196, JW3163

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the sodium/potassium/calcium exchanger family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to plasma membrane Inferred from direct assay PubMed 20196076. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Inner membrane protein YrbG		PRO_0000209507
Regions
	Topological domain	1 – 5	5	Periplasmic Potential
	Transmembrane	6 – 26	21	Helical; Potential
	Topological domain	27 – 37	11	Cytoplasmic Potential
	Transmembrane	38 – 58	21	Helical; Potential
	Topological domain	59 – 67	9	Periplasmic Potential
	Transmembrane	68 – 88	21	Helical; Potential
	Topological domain	89 – 104	16	Cytoplasmic Potential
	Transmembrane	105 – 125	21	Helical; Potential
	Topological domain	126	1	Periplasmic Potential
	Transmembrane	127 – 147	21	Helical; Potential
	Topological domain	148 – 169	22	Cytoplasmic Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Topological domain	191 – 198	8	Periplasmic Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Topological domain	220 – 243	24	Cytoplasmic Potential
	Transmembrane	244 – 264	21	Helical; Potential
	Topological domain	265 – 269	5	Periplasmic Potential
	Transmembrane	270 – 290	21	Helical; Potential
	Topological domain	291 – 302	12	Cytoplasmic Potential
	Transmembrane	303 – 323	21	Helical; Potential
	Topological domain	324 – 325	2	Periplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P45394 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: BC08E0BBFD56A6C3	FASTA	325	34,741
	10 20 30 40 50 60 MLLATALLIV GLLLVVYSAD RLVFAASILC RTFGIPPLII GMTVVSIGTS LPEVIVSLAA 70 80 90 100 110 120 SLHEQRDLAV GTALGSNIIN ILLILGLAAL VRPFTVHSDV LRRELPLMLL VSVVAGSVLY 130 140 150 160 170 180 DGQLSRSDGI FLLFLAVLWL LFIVKLARQA ERQGTDSLTR EQLAELPRDG GLPVAFLWLG 190 200 210 220 230 240 IALIIMPVAT RMVVDNATVL ANYFAISELT MGLTAIAIGT SLPELATAIA GVRKGENDIA 250 260 270 280 290 300 VGNIIGANIF NIVIVLGLPA LITPGEIDPL AYSRDYSVML LVSIIFALLC WRRSPQPGRG 310 320 VGVLLTGGFI VWLAMLYWLS PILVE

References

[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA57997.1. U00096 Genomic DNA. Translation: AAC76228.1. AP009048 Genomic DNA. Translation: BAE77240.1.
PIR	F65110.
RefSeq	NP_417663.1. NC_000913.2. YP_491381.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P45394.
SMR	P45394. Positions 19-316.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3196.
Protein family/group databases
TCDB	2.A.19.5.1. Ca2+:cation antiporter (CaCA) family.
Proteomic databases
PaxDb	P45394.
PRIDE	P45394.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76228; AAC76228; b3196. BAE77240; BAE77240; BAE77240.
GeneID	12932585. 947730.
KEGG	ecj:Y75_p3116. eco:b3196.
PATRIC	32121812. VBIEscCol129921_3290.
Organism-specific databases
EchoBASE	EB2654.
EcoGene	EG12802. yrbG.
Phylogenomic databases
eggNOG	COG0530.
HOGENOM	HOG000274121.
KO	K07301.
OMA	SADWMVQ.
ProtClustDB	PRK10734.
Enzyme and pathway databases
BioCyc	EcoCyc:YRBG-MONOMER. ECOL316407:JW3163-MONOMER.
Gene expression databases
Genevestigator	P45394.
Family and domain databases
InterPro	IPR004481. K/Na/Ca-exchanger. IPR004837. NaCa_Exmemb. [Graphical view]
PANTHER	PTHR10846. PTHR10846. 1 hit.
Pfam	PF01699. Na_Ca_ex. 2 hits. [Graphical view]
TIGRFAMs	TIGR00367. TIGR00367. 1 hit.
ProtoNet	Search...

Entry information

Entry name

YRBG_ECOLI

Accession

Primary (citable) accession number: P45394
Secondary accession number(s): Q2M916

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program