Reviewed, UniProtKB/Swiss-Prot P45386 (IGA4_HAEIN)

Names and origin

Protein names

Recommended name:
    Immunoglobulin A1 protease
      Short name=IGA1 protease
    EC=3.4.21.72

Gene names

Name:

iga

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	1849 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Virulence factor; cleaves host immunoglobulin A producing intact Fc and Fab fragments.
Catalytic activity	Cleavage of immunoglobulin A molecules at certain Pro-|-Xaa bonds in the hinge region. No small molecule substrates are known.
Subcellular location	Secreted.
Domain	The signal peptide guides the precursor to the periplasmic space, and the C-terminal helper domain associates with the outer membrane to form a pore for excretion of the protease domain. The helper domain is then released by autoproteolysis By similarity.
Sequence similarities	Contains 1 autotransporter (TC 1.B.12) domain. [View classification] Contains 1 peptidase S6 domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Membrane Secreted
Domain	Signal Transmembrane
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW outer membrane Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 1021	996	Immunoglobulin A1 protease		PRO_0000026966
	Propeptide	1022 – 1849	828	Helper peptide Potential		PRO_0000026967
Regions
	Domain	1597 – 1849	253	Autotransporter
Sites
	Active site	299	1	Probable

Sequences

	Sequence		Length	Mass (Da)
	P45386-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 79A7D018C7150AEA	FASTA	1,849	202,957
	10 20 30 40 50 60 MLNKKFKLNF IALTVAYALT PYTEAALVRD DVDYQIFRDF AENKGKFSVG ATNVEVRDKK 70 80 90 100 110 120 NQSLGSALPN GIPMIDFSVV DVDKRIATLV NPQYVVGVKH VSNGVSELHF GNLNGNMNNG 130 140 150 160 170 180 NAKSHRDVSS EENRYYTVEK NNFPTENVTS FTTKEEQDAQ KRREDYYMPR LDKFVTEVAP 190 200 210 220 230 240 IEASTANNNK GEYNNSDKYP AFVRLGSGSQ FIYKKGSRYQ LILTEKDKQG NLLRNWDVGG 250 260 270 280 290 300 DNLELVGNAY TYGIAGTPYK VNHENNGLIG FGNSKEEHSD PKGILSQDPL TNYAVLGDSG 310 320 330 340 350 360 SPLFVYDREK GKWLFLGSYD FWAGYNKKSW QEWNIYKHEF AEKIYQQYSA GSLTGSNTQY 370 380 390 400 410 420 TWQATGSTST ITGGGEPLSV DLTDGKDKPN HGKSITLKGS GTLTLNNHID QGAGGLFFEG 430 440 450 460 470 480 DYEVKGTSDS TTWKGAGVSV ADGKTVTWKV HNPKYDRLAK IGKGTLVVEG KGKNEGLLKV 490 500 510 520 530 540 GDGTVILKQK ADANNKVQAF SQVGIVSGRS TLVLNDDKQV DPNSIYFGFR GGRLDLNGNS 550 560 570 580 590 600 LTFDHIRNID DGARVVNHNM TNTSNITITG ESLITNPNTI TSYNIEAQDD DHPLRIRSIP 610 620 630 640 650 660 YRQLYFNQDN RSYYTLKKGA STRSELPQNS GESNENWLYM GRTSDEAKRN VMNHINNERM 670 680 690 700 710 720 NGFNGYFGEE ETKATQNGKL NVTFNGKSDQ NRFLLTGGTN LNGDLNVEKG TLFLSGRPTP 730 740 750 760 770 780 HARDIAGISS TKKDPHFTEN NEVVVEDDWI NRNFKATTMN VTGNASLYSG RNVANITSNI 790 800 810 820 830 840 TASNNAQVHI GYKTGDTVCV RSDYTGYVTC HNSNLSEKAL NSFNPTNLRG NVNLTENASF 850 860 870 880 890 900 TLGKANLFGT IQSIGTSQVN LKENSHWHLT GNSNVNQLNL TNGHIHLNAQ NDANKVTTYN 910 920 930 940 950 960 TLTVNSLSGN GSFYYWVDFT NNKSNKVVVN KSATGNFTLQ VADKTGEPNH NELTLFDASN 970 980 990 1000 1010 1020 ATRNNLEVTL ANGSVDRGAW KYKLRNVNGR YDLYNPEVEK RNQTVDTTNI TTPNDIQADA 1030 1040 1050 1060 1070 1080 PSAQSNNEEI ARVETPVPPP APATESAIAS EQPETRPAET AQPAMEETNT ANSTETAPKS 1090 1100 1110 1120 1130 1140 DTATQTENPN SESVPSETTE KVAENPPQEN ETVAKNEQEA TEPTPQNGEV AKEDQPTVEA 1150 1160 1170 1180 1190 1200 NTQTNEATQS EGKTEETQTA ETKSEPTESV TVSENQPEKT VSQSTEDKVV VEKEEKAKVE 1210 1220 1230 1240 1250 1260 TEETQKAPQV TSKEPPKQAE PAPEEVPTDT NAEEAQALQQ TQPTTVAAAE TTSPNSKPAE 1270 1280 1290 1300 1310 1320 ETQQPSEKTN AEPVTPVVSE NTATQPTETE ETAKVEKEKT QEVPQVASQE SPKQEQPAAK 1330 1340 1350 1360 1370 1380 PQAQTKPQAE PARENVLTTK NVGEPQPQAQ PQTQSTAVPT TGETAANSKP AAKPQAQAKP 1390 1400 1410 1420 1430 1440 QTEPARENVS TVNTKEPQSQ TSATVSTEQP AKETSSNVEQ PAPENSINTG SATTMTETAE 1450 1460 1470 1480 1490 1500 KSDKPQMETV TENDRQPEAN TVADNSVANN SESSESKSRR RRSVSQPKET SAEETTVAST 1510 1520 1530 1540 1550 1560 QETTVDNSVS TPKPRSRRTR RSVQTNSYEP VELPTENAEN AENVQSGNNV ANSQPALRNL 1570 1580 1590 1600 1610 1620 TSKNTNAVLS NAMAKAQFVA LNVGKAVSQH ISQLEMNNEG QYNVWISNTS MNKNYSSEQY 1630 1640 1650 1660 1670 1680 RRFSSKSTQT QLGWDQTISN NVQLGGVFTY VRNSNNFDKA SSKNTLAQVN FYSKYYADNH 1690 1700 1710 1720 1730 1740 WYLGIDLGYG KFQSNLQTNN NAKFARHTAQ IGLTAGKAFN LGNFAVKPTV GVRYSYLSNA 1750 1760 1770 1780 1790 1800 DFALAQDRIK VNPISVKTAF AQVDLSYTYH LGEFSITPIL SARYDANQGN GKINVSVYDF 1810 1820 1830 1840 AYNVENQQQY NAGLKLKYHN VKLSLIGGLT KAKQAEKQKT AEVKLSFSF

References

[1]	"A comparative genetic study of serologically distinct Haemophilus influenzae type 1 immunoglobulin A1 proteases." Poulsen K., Reinholdt J., Kilian M. J. Bacteriol. 174:2913-2921(1992) [PubMed: 1373717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NHTI HK61.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M87491 Genomic DNA. Translation: AAA24968.1.
PIR	C41859.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S06.007.
Enzyme and pathway databases
BRENDA	3.4.21.72. 109.
Family and domain databases
InterPro	IPR005546. Auto_transptbeta. IPR006315. OM_autotransptr_brl. IPR012332. P22_tailspike. IPR000710. Peptidase_S6. IPR004899. Pertactin. [Graphical view]
Gene3D	G3DSA:2.160.20.20. P22_tailspike. 1 hit.
Pfam	PF03797. Autotransporter. 1 hit. PF02395. Peptidase_S6. 1 hit. PF03212. Pertactin. 1 hit. [Graphical view]
PRINTS	PR00921. IGASERPTASE.
TIGRFAMs	TIGR01414. autotrans_barl. 1 hit.
PROSITE	PS51208. AUTOTRANSPORTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IGA4_HAEIN

Accession

Primary (citable) accession number: P45386

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)