Reviewed, UniProtKB/Swiss-Prot P45385 (IGA3_HAEIN)

Names and origin

Protein names

Recommended name:
    Immunoglobulin A1 protease
      Short name=IGA1 protease
    EC=3.4.21.72

Gene names

Name:

iga

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	1545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Virulence factor; cleaves host immunoglobulin A producing intact Fc and Fab fragments.
Catalytic activity	Cleavage of immunoglobulin A molecules at certain Pro-|-Xaa bonds in the hinge region. No small molecule substrates are known.
Subcellular location	Secreted.
Domain	The signal peptide guides the precursor to the periplasmic space, and the C-terminal helper domain associates with the outer membrane to form a pore for excretion of the protease domain. The helper domain is then released by autoproteolysis By similarity.
Sequence similarities	Contains 1 autotransporter (TC 1.B.12) domain. [View classification] Contains 1 peptidase S6 domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Membrane Secreted
Domain	Signal Transmembrane
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW outer membrane Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 1012	987	Immunoglobulin A1 protease		PRO_0000026964
	Propeptide	1013 – 1545	533	Helper peptide Potential		PRO_0000026965
Regions
	Domain	1293 – 1545	253	Autotransporter
Sites
	Active site	292	1	Probable

Sequences

	Sequence		Length	Mass (Da)
	P45385-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 3EDD753988F6D478	FASTA	1,545	170,627
	10 20 30 40 50 60 MLNKKFKLNF IALTVAYALT PYTEAALVRD DVDYQIFRDF AENKGKFSVG ATNVEVRDKN 70 80 90 100 110 120 NRPLGNVLPN GIPMIDFSVV DVDKRIATLV NPQYVVGVKH VSNGVSELHF GNLNGNMNNG 130 140 150 160 170 180 NAKAHRDVSS EENRYYTVEK NEYPTKLNGK AVTTEDQAQK RREDYYMPRL DKFVTEVAPI 190 200 210 220 230 240 EASTDSSTAG TYNNKDKYPY FVRLGSGTQF IYENGTRYEL WLGKEGQKSD AGGYNLKLVG 250 260 270 280 290 300 NAYTYGIAGT PYEVNHENDG LIGFGNSNNE YINPKEILSK KPLTNYAVLG DSGSPLFVYD 310 320 330 340 350 360 REKGKWLFLG SYDYWAGYNK KSWQEWNIYK PEFAEKIYEQ YSAGSLIGSK TDYSWSSNGK 370 380 390 400 410 420 TSTITGGEKS LNVDLADGKD KPNHGKSVTF EGSGTLTLNN NIDQGAGGLF FEGDYEVKGT 430 440 450 460 470 480 SDNTTWKGAG VSVAEGKTVT WKVHNPQYDR LAKIGKGTLI VEGTGDNKGS LKVGDGTVIL 490 500 510 520 530 540 KQQTNGSGQH AFASVGIVSG RSTLVLNDDK QVDPNSIYFG FRGGRLDLNG NSLTFDHIRN 550 560 570 580 590 600 IDEGARLVNH STSKHSTVTI TGDNLITDPN NVSIYYVKPL EDDNPYAIRQ IKYGYQLYFN 610 620 630 640 650 660 EENRTYYALK KDASIRSEFP QNRGESNNSW LYMGTEKADA QKNAMNHINN ERMNGFNGYF 670 680 690 700 710 720 GEEEGKNNGN LNVTFKGKSE QNRFLLTGGT NLNGDLNVQQ GTLFLSGRPT PHARDIAGIS 730 740 750 760 770 780 STKKDSHFSE NNEVVVEDDW INRNFKATNI NVTNNATLYS GRNVESITSN ITASNNAKVH 790 800 810 820 830 840 IGYKAGDTVC VRSDYTGYVT CTTDKLSDKA LNSFNPTNLR GNVNLTESAN FVLGKANLFG 850 860 870 880 890 900 TIQSRGNSQV RLTENSHWHL TGNSDVHQLD LANGHIHLNS ADNSNNVTKY NTLTVNSLSG 910 920 930 940 950 960 NGSFYYLTDL SNKQGDKVVV TKSATGNFTL QVADKTGEPN HNELTLFDAS KAQRDHLNVS 970 980 990 1000 1010 1020 LVGNTVDLGA WKYKLRNVNG RYDLYNPEVE KRNQTVDTTN ITTPNNIQAD VPSVPSNNEE 1030 1040 1050 1060 1070 1080 IARVDEAPVP PPAPATPSET TETVAENSKQ ESKTVEKNEQ DATETTAQNR EVAKEAKSNV 1090 1100 1110 1120 1130 1140 KANTQTNEVA QSGSETKETQ TTETKETATV EKEEKAKVET EKTQEVPKVT SQVSPKQEQS 1150 1160 1170 1180 1190 1200 ETVQPQAEPA RENDPTVNIK EPQSQTNTTA DTEQPAKETS SNVEQPVTES TTVNTGNSVV 1210 1220 1230 1240 1250 1260 ENPENTTPAT TQPTVNSESS NKPKNRHRRS VRSVPHNVEP ATTSSNDRST VALCDLTSTN 1270 1280 1290 1300 1310 1320 TNAVLSDARA KAQFVALNVG KAVSQHISQL EMNNEGQYNV WVSNTSMNKN YSSSQYRRFS 1330 1340 1350 1360 1370 1380 SKSTQTQLGW DQTISNNVQL GGVFTYVRNS NNFDKATSKN TLAQVNFYSK YYADNHWYLG 1390 1400 1410 1420 1430 1440 IDLGYGKFQS KLQTNHNAKF ARHTAQFGLT AGKAFNLGNF GITPIVGVRY SYLSNADFAL 1450 1460 1470 1480 1490 1500 DQARIKVNPI SVKTAFAQVD LSYTYHLGEF SVTPILSARY DANQGSGKIN VNGYDFAYNV 1510 1520 1530 1540 ENQQQYNAGL KLKYHNVKLS LIGGLTKAKQ AEKQKTAELK LSFSF

References

[1]	"A comparative genetic study of serologically distinct Haemophilus influenzae type 1 immunoglobulin A1 proteases." Poulsen K., Reinholdt J., Kilian M. J. Bacteriol. 174:2913-2921(1992) [PubMed: 1373717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HK393 / NCTC 8467 / Serotype B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M87490 Genomic DNA. Translation: AAA24967.1.
PIR	B41859.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.72. 109.
Family and domain databases
InterPro	IPR005546. Auto_transptbeta. IPR006315. OM_autotransptr_brl. IPR012332. P22_tailspike. IPR000710. Peptidase_S6. IPR004899. Pertactin. [Graphical view]
Gene3D	G3DSA:2.160.20.20. P22_tailspike. 1 hit.
Pfam	PF03797. Autotransporter. 1 hit. PF02395. Peptidase_S6. 1 hit. PF03212. Pertactin. 1 hit. [Graphical view]
PRINTS	PR00921. IGASERPTASE.
TIGRFAMs	TIGR01414. autotrans_barl. 1 hit.
PROSITE	PS51208. AUTOTRANSPORTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IGA3_HAEIN

Accession

Primary (citable) accession number: P45385

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)