Reviewed, UniProtKB/Swiss-Prot P45384 (IGA2_HAEIN)

Names and origin

Protein names

Recommended name:
    Immunoglobulin A1 protease
      Short name=IGA1 protease
    EC=3.4.21.72

Gene names

Name:

iga

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	1702 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Virulence factor; cleaves host immunoglobulin A producing intact Fc and Fab fragments.
Catalytic activity	Cleavage of immunoglobulin A molecules at certain Pro-|-Xaa bonds in the hinge region. No small molecule substrates are known.
Subcellular location	Secreted.
Domain	The signal peptide guides the precursor to the periplasmic space, and the C-terminal helper domain associates with the outer membrane to form a pore for excretion of the protease domain. The helper domain is then released by autoproteolysis By similarity.
Sequence similarities	Contains 1 autotransporter (TC 1.B.12) domain. [View classification] Contains 1 peptidase S6 domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Membrane Secreted
Domain	Repeat Signal Transmembrane
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW outer membrane Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 1014	989	Immunoglobulin A1 protease		PRO_0000026962
	Propeptide	1015 – 1702	688	Helper peptide Potential		PRO_0000026963
Regions
	Repeat	1109 – 1116	8	1
	Repeat	1117 – 1124	8	2
	Domain	1450 – 1702	253	Autotransporter
	Region	1109 – 1124	16	2 X 8 AA tandem repeats of A-K-V-E-K-E-E-K
Sites
	Active site	288	1	Probable

Sequences

	Sequence		Length	Mass (Da)
	P45384-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 860F70D2667807A6	FASTA	1,702	186,539
	10 20 30 40 50 60 MLNKKFKLNF IALTVAYALT PYTEAALVRD DVDYQIFRDF AENKGRFSVG ATNVEVRDKN 70 80 90 100 110 120 NHSLGNVLPN GIPMIDFSVV DVDKRIATLI NPQYVVGVKH VSNGVSELHF GNLNGNMNNG 130 140 150 160 170 180 NDKSHRDVSS EENRYFSVEK NEYPTKLNGK AVTTEDQTQK RREDYYMPRL DKFVTEVAPI 190 200 210 220 230 240 EASTASSDAG TYNDQNKYPA FVRLGSGSQF IYKKGDNYSL ILNNHEVGGN NLKLVGDAYT 250 260 270 280 290 300 YGIAGTPYKV NHENNGLIGF GNSKEEHSDP KGILSQDPLT NYAVLGDSGS PLFVYDREKG 310 320 330 340 350 360 KWLFLGSYDF WAGYNKKSWQ EWNIYKPEFA KTVLDKDTAG SLTGSNTQYN WNPTGKTSVI 370 380 390 400 410 420 SNGSESLNVD LFDSSQDTDS KKNNHGKSVT LRGSGTLTLN NNIDQGAGGL FFEGDYEVKG 430 440 450 460 470 480 TSDSTTWKGA GVSVADGKTV TWKVHNPKSD RLAKIGKGTL IVEGKGENKG SLKVGDGTVI 490 500 510 520 530 540 LKQQADANNK VKAFSQVGIV SGRSTVVLND DKQVDPNSIY FGFRGGRLDA NGNNLTFEHI 550 560 570 580 590 600 RNIDDGARLV NHNTSKTSTV TITGESLITD PNTITPYNID APDEDNPYAF RRIKDGGQLY 610 620 630 640 650 660 LNLENYTYYA LRKGASTRSE LPKNSGESNE NWLYMGKTSD EAKRNVMNHI NNERMNGFNG 670 680 690 700 710 720 YFGEEEGKNN GNLNVTFKGK SEQNRFLLTG GTNLNGDLKV EKGTLFLSGR PTPHARDIAG 730 740 750 760 770 780 ISSTKKDQHF AENNEVVVED DWINRNFKAT NINVTNNATL YSGRNVANIT SNITASDNAK 790 800 810 820 830 840 VHIGYKAGDT VCVRSDYTGY VTCTTDKLSD KALNSFNATN VSGNVNLSGN ANFVLGKANL 850 860 870 880 890 900 FGTISGTGNS QVRLTENSHW HLTGDSNVNQ LNLDKGHIHL NAQNDANKVT TYNTLTVNSL 910 920 930 940 950 960 SGNGSFYYLT DLSNKQGDKV VVTKSATGNF TLQVADKTGE PTKNELTLFD ASNATRNNLN 970 980 990 1000 1010 1020 VSLVGNTVDL GAWKYKLRNV NGRYDLYNPE VEKRNQTVDT TNITTPNNIQ ADVPSVPSNN 1030 1040 1050 1060 1070 1080 EEIARVETPV PPPAPATPSE TTETVAENSK QESKTVEKNE QDATETTAQN GEVAEEAKPS 1090 1100 1110 1120 1130 1140 VKANTQTNEV AQSGSETEET QTTEIKETAK VEKEEKAKVE KEEKAKVEKD EIQEAPQMAS 1150 1160 1170 1180 1190 1200 ETSPKQAKPA PKEVSTDTKV EETQVQAQPQ TQSTTVAAAE ATSPNSKPAE ETQPSEKTNA 1210 1220 1230 1240 1250 1260 EPVTPVVSKN QTENTTDQPT EREKTAKVET EKTQEPPQVA SQASPKQEQS ETVQPQAVLE 1270 1280 1290 1300 1310 1320 SENVPTVNNA EEVQAQLQTQ TSATVSTKQP APENSINTGS ATAITETAEK SDKPQTETAA 1330 1340 1350 1360 1370 1380 STEDASQHKA NTVADNSVAN NSESSEPKSR RRRSISQPQE TSAEETTAAS TDETTIADNS 1390 1400 1410 1420 1430 1440 KRSKPNRRSR RSVRSEPTVT NGSDRSTVAL RDLTSTNTNA VISDAMAKAQ FVALNVGKAV 1450 1460 1470 1480 1490 1500 SQHISQLEMN NEGQYNVWVS NTSMNENYSS SQYRRFSSKS TQTQLGWDQT ISNNVQLGGV 1510 1520 1530 1540 1550 1560 FTYVRNSNNF DKASSKNTLA QVNFYSKYYA DNHWYLGIDL GYGKFQSNLK TNHNAKFARH 1570 1580 1590 1600 1610 1620 TAQFGLTAGK AFNLGNFGIT PIVGVRYSYL SNANFALAKD RIKVNPISVK TAFAQVDLSY 1630 1640 1650 1660 1670 1680 TYHLGEFSVT PILSARYDTN QGSGKINVNQ YDFAYNVENQ QQYNAGLKLK YHNVKLSLIG 1690 1700 GLTKAKQAEK QKTAELKLSF SF

References

[1]	"A comparative genetic study of serologically distinct Haemophilus influenzae type 1 immunoglobulin A1 proteases." Poulsen K., Reinholdt J., Kilian M. J. Bacteriol. 174:2913-2921(1992) [PubMed: 1373717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HK715 / Serotype B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M87489 Genomic DNA. Translation: AAA24966.1.
PIR	A41859.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.72. 109.
Family and domain databases
InterPro	IPR005546. Auto_transptbeta. IPR006315. OM_autotransptr_brl. IPR012332. P22_tailspike. IPR000710. Peptidase_S6. IPR004899. Pertactin. [Graphical view]
Gene3D	G3DSA:2.160.20.20. P22_tailspike. 1 hit.
Pfam	PF03797. Autotransporter. 1 hit. PF02395. Peptidase_S6. 1 hit. PF03212. Pertactin. 1 hit. [Graphical view]
PRINTS	PR00921. IGASERPTASE.
TIGRFAMs	TIGR01414. autotrans_barl. 1 hit.
PROSITE	PS51208. AUTOTRANSPORTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IGA2_HAEIN

Accession

Primary (citable) accession number: P45384

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)