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Protein

Aldose reductase-related protein 2

Gene

Akr1b8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Alditol + NAD(P)+ = aldose + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Proton donor
Sitei78 – 781Lowers pKa of active site TyrBy similarity
Binding sitei111 – 1111Substrate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi211 – 27363NADP1 PublicationAdd
BLAST

GO - Molecular functioni

  1. alditol:NADP+ 1-oxidoreductase activity Source: UniProtKB-EC
  2. geranylgeranyl reductase activity Source: MGI
  3. indanol dehydrogenase activity Source: MGI
  4. retinal dehydrogenase activity Source: MGI

GO - Biological processi

  1. daunorubicin metabolic process Source: MGI
  2. doxorubicin metabolic process Source: MGI
  3. farnesol catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_198569. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldose reductase-related protein 2 (EC:1.1.1.21)
Short name:
AR
Alternative name(s):
Aldehyde reductase
Fibroblast growth factor-regulated protein
Protein FR-1
Gene namesi
Name:Akr1b8
Synonyms:Fgfrp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:107673. Akr1b8.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Aldose reductase-related protein 2PRO_0000124631Add
BLAST

Proteomic databases

MaxQBiP45377.
PaxDbiP45377.
PRIDEiP45377.

2D gel databases

REPRODUCTION-2DPAGEIPI00273096.
P45377.

PTM databases

PhosphoSiteiP45377.

Expressioni

Inductioni

By FGF-1.

Gene expression databases

BgeeiP45377.
GenevestigatoriP45377.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiP45377. 1 interaction.
MINTiMINT-4087589.
STRINGi10090.ENSMUSP00000040244.

Structurei

Secondary structure

1
316
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Beta strandi12 – 165Combined sources
Helixi25 – 3713Combined sources
Beta strandi42 – 443Combined sources
Helixi47 – 493Combined sources
Helixi52 – 6413Combined sources
Helixi70 – 723Combined sources
Beta strandi74 – 796Combined sources
Helixi81 – 833Combined sources
Helixi86 – 10015Combined sources
Beta strandi105 – 1106Combined sources
Helixi138 – 15013Combined sources
Beta strandi153 – 1619Combined sources
Helixi164 – 1718Combined sources
Beta strandi182 – 1865Combined sources
Helixi194 – 2029Combined sources
Beta strandi206 – 2116Combined sources
Turni228 – 2303Combined sources
Helixi232 – 2409Combined sources
Helixi245 – 25410Combined sources
Turni255 – 2573Combined sources
Helixi267 – 2748Combined sources
Helixi283 – 2908Combined sources
Helixi302 – 3043Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FRBX-ray1.70A2-316[»]
ProteinModelPortaliP45377.
SMRiP45377. Positions 2-315.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45377.

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiCOG0656.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP45377.
KOiK00011.
OMAiVSNFSHF.
OrthoDBiEOG70KGQF.
PhylomeDBiP45377.
TreeFamiTF106492.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATFVELSTK AKMPIVGLGT WKSPPNQVKE AVKAAIDAGY RHIDCAYAYC
60 70 80 90 100
NENEVGEAIQ EKIKEKAVQR EDLFIVSKLW PTCFEKKLLK EAFQKTLTDL
110 120 130 140 150
KLDYLDLYLI HWPQGLQPGK ELFPKDDQGR ILTSKTTFLE AWEGMEELVD
160 170 180 190 200
QGLVKALGVS NFNHFQIERL LNKPGLKHKP VTNQVECHPY LTQEKLIQYC
210 220 230 240 250
HSKGISVTAY SPLGSPDRPS AKPEDPSLLE DPKIKEIAAK HEKTSAQVLI
260 270 280 290 300
RFHIQRNVVV IPKSVTPSRI QENIQVFDFQ LSDEEMATIL SFNRNWRACL
310
LPETVNMEEY PYDAEY
Length:316
Mass (Da):36,121
Last modified:January 23, 2007 - v2
Checksum:i0C6F0A7BA806497C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti242 – 2421E → K in AAH05789 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04204 mRNA. Translation: AAA16953.1.
BC005789 mRNA. Translation: AAH05789.1.
CCDSiCCDS19991.1.
PIRiA53440.
RefSeqiNP_032038.1. NM_008012.1.
UniGeneiMm.5378.

Genome annotation databases

EnsembliENSMUST00000038406; ENSMUSP00000040244; ENSMUSG00000029762.
GeneIDi14187.
KEGGimmu:14187.
UCSCiuc009bgz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04204 mRNA. Translation: AAA16953.1.
BC005789 mRNA. Translation: AAH05789.1.
CCDSiCCDS19991.1.
PIRiA53440.
RefSeqiNP_032038.1. NM_008012.1.
UniGeneiMm.5378.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FRBX-ray1.70A2-316[»]
ProteinModelPortaliP45377.
SMRiP45377. Positions 2-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP45377. 1 interaction.
MINTiMINT-4087589.
STRINGi10090.ENSMUSP00000040244.

PTM databases

PhosphoSiteiP45377.

2D gel databases

REPRODUCTION-2DPAGEIPI00273096.
P45377.

Proteomic databases

MaxQBiP45377.
PaxDbiP45377.
PRIDEiP45377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038406; ENSMUSP00000040244; ENSMUSG00000029762.
GeneIDi14187.
KEGGimmu:14187.
UCSCiuc009bgz.1. mouse.

Organism-specific databases

CTDi14187.
MGIiMGI:107673. Akr1b8.

Phylogenomic databases

eggNOGiCOG0656.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP45377.
KOiK00011.
OMAiVSNFSHF.
OrthoDBiEOG70KGQF.
PhylomeDBiP45377.
TreeFamiTF106492.

Enzyme and pathway databases

ReactomeiREACT_198569. Retinoid metabolism and transport.

Miscellaneous databases

EvolutionaryTraceiP45377.
NextBioi285402.
PROiP45377.
SOURCEiSearch...

Gene expression databases

BgeeiP45377.
GenevestigatoriP45377.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A delayed-early gene activated by fibroblast growth factor-1 encodes a protein related to aldose reductase."
    Donohue P.J., Alberts G.F., Hampton B.S., Winkles J.A.
    J. Biol. Chem. 269:8604-8609(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "1.7-A structure of FR-1, a fibroblast growth factor-induced member of the aldo-keto reductase family, complexed with coenzyme and inhibitor."
    Wilson D.K., Nakano T., Petrash M., Quiocho F.A.
    Biochemistry 34:14323-14330(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH NADPH AND INHIBITOR.

Entry informationi

Entry nameiALD2_MOUSE
AccessioniPrimary (citable) accession number: P45377
Secondary accession number(s): Q99JN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.