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P45376

- ALDR_MOUSE

UniProt

P45376 - ALDR_MOUSE

Protein

Aldose reductase

Gene

Akr1b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

    Catalytic activityi

    Alditol + NAD(P)+ = aldose + NAD(P)H.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei49 – 491Proton donorBy similarity
    Sitei78 – 781Lowers pKa of active site TyrBy similarity
    Binding sitei111 – 1111SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 1910NADPSequence Analysis
    Nucleotide bindingi211 – 27363NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. alditol:NADP+ 1-oxidoreductase activity Source: MGI
    2. glyceraldehyde oxidoreductase activity Source: Ensembl

    GO - Biological processi

    1. daunorubicin metabolic process Source: Ensembl
    2. doxorubicin metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_223561. Pregnenolone biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldose reductase (EC:1.1.1.21)
    Short name:
    AR
    Alternative name(s):
    Aldehyde reductase
    Gene namesi
    Name:Akr1b1
    Synonyms:Akr1b3, Aldor1, Aldr1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1353494. Akr1b3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 316315Aldose reductasePRO_0000124624Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei95 – 951N6-acetyllysineBy similarity
    Modified residuei222 – 2221N6-acetyllysineBy similarity
    Modified residuei263 – 2631N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP45376.
    PaxDbiP45376.
    PRIDEiP45376.

    2D gel databases

    COMPLUYEAST-2DPAGEP45376.
    REPRODUCTION-2DPAGEIPI00223757.
    P45376.
    SWISS-2DPAGEP45376.

    PTM databases

    PhosphoSiteiP45376.

    Expressioni

    Tissue specificityi

    Abundant in the testis, skeletal muscle and kidney.

    Gene expression databases

    BgeeiP45376.
    GenevestigatoriP45376.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi198069. 2 interactions.
    IntActiP45376. 6 interactions.
    MINTiMINT-1869603.

    Structurei

    3D structure databases

    ProteinModelPortaliP45376.
    SMRiP45376. Positions 2-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiCOG0656.
    HOGENOMiHOG000250272.
    HOVERGENiHBG000020.
    InParanoidiP45376.
    KOiK00011.
    OMAiDFLDTWT.
    OrthoDBiEOG70KGQF.
    PhylomeDBiP45376.
    TreeFamiTF106492.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P45376-1 [UniParc]FASTAAdd to Basket

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    MASHLELNNG TKMPTLGLGT WKSPPGQVTE AVKVAIDLGY RHIDCAQVYQ    50
    NEKEVGVALQ EKLKEQVVKR QDLFIVSKLW CTFHDKSMVK GAFQKTLSDL 100
    QLDYLDLYLI HWPTGFKPGP DYFPLDASGN VIPSDTDFVD TWTAMEQLVD 150
    EGLVKTIGVS NFNPLQIERI LNKPGLKYKP AVNQIECHPY LTQEKLIEYC 200
    HSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK YNKTTAQVLI 250
    RFPIQRNLVV IPKSVTPVRI AENLKVFDFE VSSEDMATLL SYNRNWRVCA 300
    LMSCAKHKDY PFHAEV 316
    Length:316
    Mass (Da):35,732
    Last modified:January 23, 2007 - v3
    Checksum:iE18759AD160B4A0E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461A → S in BAA06980. (PubMed:7851421)Curated
    Sequence conflicti221 – 2211A → G in AAC13358. (PubMed:9485485)Curated
    Sequence conflicti281 – 2811V → L in AAH04725. (PubMed:15489334)Curated
    Sequence conflicti281 – 2811V → L in AAH21655. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D32250 mRNA. Translation: BAA06980.1.
    L39795 mRNA. Translation: AAA62176.1.
    U29152 mRNA. Translation: AAA69958.1.
    U89150
    , U89140, U89142, U89143, U89144, U89145, U89146, U89147, U89148, U89149 Genomic DNA. Translation: AAC13358.1.
    U93231, U93230 Genomic DNA. Translation: AAD32300.1.
    AB016665 Genomic DNA. Translation: BAA76413.1.
    BC004725 mRNA. Translation: AAH04725.1.
    BC021655 mRNA. Translation: AAH21655.1.
    CCDSiCCDS19990.1.
    PIRiI49484.
    RefSeqiNP_033788.3. NM_009658.3.
    UniGeneiMm.389126.
    Mm.451.

    Genome annotation databases

    EnsembliENSMUST00000102980; ENSMUSP00000100045; ENSMUSG00000001642.
    GeneIDi11677.
    KEGGimmu:11677.
    UCSCiuc009bgy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D32250 mRNA. Translation: BAA06980.1 .
    L39795 mRNA. Translation: AAA62176.1 .
    U29152 mRNA. Translation: AAA69958.1 .
    U89150
    , U89140 , U89142 , U89143 , U89144 , U89145 , U89146 , U89147 , U89148 , U89149 Genomic DNA. Translation: AAC13358.1 .
    U93231 , U93230 Genomic DNA. Translation: AAD32300.1 .
    AB016665 Genomic DNA. Translation: BAA76413.1 .
    BC004725 mRNA. Translation: AAH04725.1 .
    BC021655 mRNA. Translation: AAH21655.1 .
    CCDSi CCDS19990.1.
    PIRi I49484.
    RefSeqi NP_033788.3. NM_009658.3.
    UniGenei Mm.389126.
    Mm.451.

    3D structure databases

    ProteinModelPortali P45376.
    SMRi P45376. Positions 2-315.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198069. 2 interactions.
    IntActi P45376. 6 interactions.
    MINTi MINT-1869603.

    PTM databases

    PhosphoSitei P45376.

    2D gel databases

    COMPLUYEAST-2DPAGE P45376.
    REPRODUCTION-2DPAGE IPI00223757.
    P45376.
    SWISS-2DPAGE P45376.

    Proteomic databases

    MaxQBi P45376.
    PaxDbi P45376.
    PRIDEi P45376.

    Protocols and materials databases

    DNASUi 11677.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102980 ; ENSMUSP00000100045 ; ENSMUSG00000001642 .
    GeneIDi 11677.
    KEGGi mmu:11677.
    UCSCi uc009bgy.2. mouse.

    Organism-specific databases

    CTDi 11677.
    MGIi MGI:1353494. Akr1b3.

    Phylogenomic databases

    eggNOGi COG0656.
    HOGENOMi HOG000250272.
    HOVERGENi HBG000020.
    InParanoidi P45376.
    KOi K00011.
    OMAi DFLDTWT.
    OrthoDBi EOG70KGQF.
    PhylomeDBi P45376.
    TreeFami TF106492.

    Enzyme and pathway databases

    Reactomei REACT_223561. Pregnenolone biosynthesis.

    Miscellaneous databases

    NextBioi 279311.
    PROi P45376.
    SOURCEi Search...

    Gene expression databases

    Bgeei P45376.
    Genevestigatori P45376.

    Family and domain databases

    Gene3Di 3.20.20.100. 1 hit.
    InterProi IPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view ]
    PANTHERi PTHR11732. PTHR11732. 1 hit.
    Pfami PF00248. Aldo_ket_red. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000097. AKR. 1 hit.
    PRINTSi PR00069. ALDKETRDTASE.
    SUPFAMi SSF51430. SSF51430. 1 hit.
    PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Presence of a closely related subgroup in the aldo-ketoreductase family of the mouse."
      Gui T., Tanimoto T., Kokai Y., Nishimura C.
      Eur. J. Biochem. 227:448-453(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Kidney.
    2. Iwata T., Carper D.
      Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR X Swiss Webster.
      Tissue: Liver.
    3. Daoudal S., Berger M., Pailhoux E., Tournaire C., Veyssiere G., Jean C.
      Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CD-1.
      Tissue: Kidney.
    4. "Characterization of the mouse aldose reductase gene and promoter in a lens epithelial cell line."
      McGowan M.H., Iwata T., Carper D.A.
      Mol. Vis. 4:2-2(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Ola.
    5. "Comparisons of genomic structures and chromosomal locations of the mouse aldose reductase and aldose reductase-like genes."
      Ho H.T.B., Jenkins N.A., Copeland N.G., Gilbert D.J., Winkles J.A., Louie H.W.Y., Lee F.K., Chung S.S.M., Chung S.K.
      Eur. J. Biochem. 259:726-730(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    6. "Characterization of genomic regions directing the cell-specific expression of the mouse aldose reductase gene."
      Li H., Nobukuni Y., Gui T., Yabe-Nishimura C.
      Biochem. Biophys. Res. Commun. 255:759-764(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    8. Lubec G., Yang J.W., Zigmond M.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 156-169.
      Tissue: Brain.

    Entry informationi

    Entry nameiALDR_MOUSE
    AccessioniPrimary (citable) accession number: P45376
    Secondary accession number(s): O70130, Q99KC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 127 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3