Reviewed, UniProtKB/Swiss-Prot P45369 (THIL_CHRVI)

Names and origin

Protein names

Recommended name:
    Acetyl-CoA acetyltransferase
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase

Gene names

Name:

phbA

Organism

Chromatium vinosum (Allochromatium vinosum)

Taxonomic identifier

1049 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Allochromatium

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	PHB biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	poly-hydroxybutyrate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Acetyl-CoA acetyltransferase		PRO_0000206419
Sites
	Active site	89	1	Acyl-thioester intermediate By similarity
	Active site	350	1	Proton acceptor By similarity
	Active site	380	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45369-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 95AC363A270F414A	FASTA	394	41,053
	10 20 30 40 50 60 MSENIVIVDA GRSAIGTFGG SLSSLSATEI GTAVLKGLLA RTGLAPEQID EVILGQVLTA 70 80 90 100 110 120 GVGQNPARQT TLHAGLPHSV PAMTINKVCG SGLKAVHLAM QAIACGDADI VIAGGQESMS 130 140 150 160 170 180 QSSHVLPRSR DGQRMGDWSM KDTMIVDGLW DAFNNYHMGT TAENIAQKYG FTREQQDAFA 190 200 210 220 230 240 AASQQKTEAA QKAGRFQDEI IPIEIPQRKG DPKVFDADEF PRHGTTAESL GKLRPAFSKD 250 260 270 280 290 300 GSVTAGNASG INDGAAMVVV MKESKAKELG LKPMARLVAF ASAGVDPAIM GTGPIPASTK 310 320 330 340 350 360 CLEKAGWTPA DLDLIEANEA FAAQAMSVNQ DMGWDLSKVN VNGGAIAIGH PIGASGARVL 370 380 390 VTLLYEMQKR DAKKGLATLC IGGGQGVALA VERM

References

[1]

"Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D." Liebergesell M., Steinbuechel A. Eur. J. Biochem. 209:135-150(1992) [PubMed: 1396692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17899 / DSM 180 / D.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L01112 Genomic DNA. Translation: AAA23322.1.
PIR	S29276.
3D structure databases
HSSP	HSSP built from PDB template 1M4T based on UniProtKB P07097.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.9. 2799.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIL_CHRVI

Accession

Primary (citable) accession number: P45369

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 22, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)