P45363 (THIL_THIVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA acetyltransferase
EC=2.3.1.9

Alternative name(s):
Acetoacetyl-CoA thiolase

Gene names

Name:

phbA

Organism

Thiocystis violacea

Taxonomic identifier

13725 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Thiocystis

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	PHB biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	poly-hydroxybutyrate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Acetyl-CoA acetyltransferase		PRO_0000206462
Sites
	Active site	89	1	Acyl-thioester intermediate By similarity
	Active site	350	1	Proton acceptor By similarity
	Active site	380	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45363 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D51EAAE97F55E3F2	FASTA	394	40,897
	10 20 30 40 50 60 MSDTIVIVDA GRTAIGTFGG ALSALQATDI GTTVLKALIE RTGIAPEQVS EVILGQVLTA 70 80 90 100 110 120 GCGQNPARQT TLMAGLPHTV PAMTINKVCG SGLKAVHLAM QAVACGDAEI VIAGGQESMS 130 140 150 160 170 180 QSSHVLPRSR EGQRMGDWPM KDTMIVDGLW DAFNQCHMGV TAENIAKKYA FTREAQDAFA 190 200 210 220 230 240 AASQQKAEAA IQSGRFADEI IPVSIPQRKG DPLVFDTDEF PRPGTTAETL GRLRPAFDKQ 250 260 270 280 290 300 GTVTAGNASG INDGAAMVVV MKESKAKELG LTPMARLVAF SSAGVDPAIM GTGPIPASTD 310 320 330 340 350 360 CLKKAGWAPA DLDLVEANEA FAAQAMSVNQ EMGWDLSKVN VNGGAIAIGH PIGASGARVL 370 380 390 VTLLYEMQKR DAKKGLATLC IGGGQGVALA VERL

References

[1]	"Cloning and molecular analysis of the poly(3-hydroxybutyric acid) biosynthetic genes of Thiocystis violacea." Liebergesell M., Steinbuechel A. Appl. Microbiol. Biotechnol. 38:493-501(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2311 / DSM 208.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L01113 Genomic DNA. Translation: AAB02860.1. S54369 Genomic DNA. Translation: AAC60428.2.
PIR	B48376.
3D structure databases
ProteinModelPortal	P45363.
SMR	P45363. Positions 4-394.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00058; UER00101.
Family and domain databases
Gene3D	3.40.47.10. 4 hits.
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
PANTHER	PTHR18919. PTHR18919. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIL_THIVI

Accession

Primary (citable) accession number: P45363

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program