Reviewed, UniProtKB/Swiss-Prot P45362 (THL_CLODI)

Names and origin

Protein names

Recommended name:
    Acetyl-CoA acetyltransferase
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase

Gene names

Name:

thi

Organism

Clostridium difficile

Taxonomic identifier

1496 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	107 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›107	›107	Acetyl-CoA acetyltransferase		PRO_0000206404
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
Experimental info
	Non-terminal residue	107	1

Sequences

	Sequence		Length	Mass (Da)
	P45362-1 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: CE702BBFB1AF0713	FASTA	107	11,101
	10 20 30 40 50 60 MREVVIASAA RTAVGSFGGA FKSVSAVELG VTAAKEAIKR ANITPDMIDE SLLGGVLTAG 70 80 90 100 LGQNIARQIA LGAGIPVEKP AMTINIVCGS GLRSWRALRT RTGPRRR

References

[1]

"Genes encoding homologues of three consecutive enzymes in the butyrate/butanol-producing pathway of Clostridium acetobutylicum are clustered on the Clostridium difficile chromosome." Mullany P., Clayton C.L., Pallen M.J., Slone R., Al-Saleh A., Tabaqchali S. FEMS Microbiol. Lett. 124:61-67(1994) [PubMed: 8001771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79899 Genomic DNA. Translation: CAA56273.1. Different initiation.
PIR	I40680.
3D structure databases
HSSP	HSSP built from PDB template 1DLU based on UniProtKB P07097.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.9. 20175.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF00108. Thiolase_N. 1 hit. [Graphical view]
PROSITE	PS00098. THIOLASE_1. Partial match. PS00737. THIOLASE_2. Partial match. PS00099. THIOLASE_3. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THL_CLODI

Accession

Primary (citable) accession number: P45362

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 9, 2007
Last modified:	September 22, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)