P45362 (THL_CLODI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA acetyltransferase
EC=2.3.1.9

Alternative name(s):
Acetoacetyl-CoA thiolase

Gene names

Name:

thi

Organism

Clostridium difficile

Taxonomic identifier

1496 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	107 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the thiolase family.
Sequence caution	The sequence CAA56273.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›107	›107	Acetyl-CoA acetyltransferase		PRO_0000206404
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
Experimental info
	Non-terminal residue	107	1

Sequences

	Sequence		Length	Mass (Da)
	P45362 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: CE702BBFB1AF0713	FASTA	107	11,101
	10 20 30 40 50 60 MREVVIASAA RTAVGSFGGA FKSVSAVELG VTAAKEAIKR ANITPDMIDE SLLGGVLTAG 70 80 90 100 LGQNIARQIA LGAGIPVEKP AMTINIVCGS GLRSWRALRT RTGPRRR

References

[1]	"Genes encoding homologues of three consecutive enzymes in the butyrate/butanol-producing pathway of Clostridium acetobutylicum are clustered on the Clostridium difficile chromosome." Mullany P., Clayton C.L., Pallen M.J., Slone R., Al-Saleh A., Tabaqchali S. FEMS Microbiol. Lett. 124:61-67(1994) [PubMed: 8001771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79899 Genomic DNA. Translation: CAA56273.1. Different initiation.
PIR	I40680.
3D structure databases
ProteinModelPortal	P45362.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF00108. Thiolase_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
PROSITE	PS00098. THIOLASE_1. Partial match. PS00737. THIOLASE_2. Partial match. PS00099. THIOLASE_3. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THL_CLODI

Accession

Primary (citable) accession number: P45362

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 9, 2007
Last modified:	September 21, 2011
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program