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P45353

- HIS2_PICPA

UniProt

P45353 - HIS2_PICPA

Protein

Histidine biosynthesis trifunctional protein

Gene

HIS4

Organism
Komagataella pastoris (Yeast) (Pichia pastoris)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.
    1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.
    L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.

    Cofactori

    Binds 1 zinc ion.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi667 – 6671ZincBy similarity
    Metal bindingi670 – 6701ZincBy similarity
    Active sitei736 – 7361By similarity
    Active sitei737 – 7371By similarity
    Metal bindingi769 – 7691ZincBy similarity
    Metal bindingi828 – 8281ZincBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. histidinol dehydrogenase activity Source: UniProtKB-EC
    3. NAD binding Source: InterPro
    4. phosphoribosyl-AMP cyclohydrolase activity Source: UniProtKB-EC
    5. phosphoribosyl-ATP diphosphatase activity Source: UniProtKB-EC
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. histidine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase, Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Histidine biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, NAD, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00031; UER00007.
    UPA00031; UER00008.
    UPA00031; UER00014.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histidine biosynthesis trifunctional protein
    Including the following 3 domains:
    Phosphoribosyl-AMP cyclohydrolase (EC:3.5.4.19)
    Phosphoribosyl-ATP pyrophosphohydrolase (EC:3.6.1.31)
    Histidinol dehydrogenase (EC:1.1.1.23)
    Short name:
    HDH
    Gene namesi
    Name:HIS4
    OrganismiKomagataella pastoris (Yeast) (Pichia pastoris)
    Taxonomic identifieri4922 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 842842Histidine biosynthesis trifunctional proteinPRO_0000135912Add
    BLAST

    Proteomic databases

    PRIDEiP45353.

    Structurei

    3D structure databases

    ProteinModelPortaliP45353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 275275Phosphoribosyl-AMP cyclohydrolaseAdd
    BLAST
    Regioni276 – 35782Phosphoribosyl-ATP pyrophosphohydrolaseAdd
    BLAST
    Regioni358 – 842485Histidinol dehydrogenaseAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the histidinol dehydrogenase family.Curated

    Family and domain databases

    HAMAPiMF_01024. HisD.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016298. Histidine_synth_trifunct.
    IPR001692. Histidinol_DH_CS.
    IPR012131. Hstdl_DH.
    IPR008179. PRib-ATP_PPHydrolase.
    IPR021130. PRib-ATP_PPHydrolase-like.
    IPR002496. PRib_AMP_CycHydrolase_dom.
    [Graphical view]
    PfamiPF00815. Histidinol_dh. 1 hit.
    PF01502. PRA-CH. 1 hit.
    PF01503. PRA-PH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001257. His_trifunctional. 1 hit.
    PRINTSiPR00083. HOLDHDRGNASE.
    ProDomiPD002610. PRA_CycHdrlase. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR00069. hisD. 1 hit.
    TIGR03188. histidine_hisI. 1 hit.
    PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45353-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTFPLLPAYA SVAEFDNSLS LVGKAVFPYA ADQLHNLIKF TQSTELQVNV    50
    QVESSVTEDQ FEELIDNLLK LYNNGINEVI LDLDLAERVV QRIPGARVIY 100
    RTLVDKVASL PANASIAVPF SSPLGDLKSF TNGGSRTVYA FSETAKLVDV 150
    TSTVASGIIP IIDARQLTTE YELSEDVKKF PVSEILLASL TTDRPDGLFT 200
    TLVADSSNYS LGLVYSSKKS IPEAIRTQTG VYQSRRHGLW YKGATSGATQ 250
    KLLGIELDCD GDCLKFVVEQ TGVGFCHLER TSCFGQSKGL RAMEATLWDR 300
    KSNAPEGSYT KRLFDDEVLL NAKIREEADE LAEAKSKEDI AWECADLFYF 350
    ALVRCAKYGV TLDEVERNLD MKSLKVTRRK GDAKPGYTKE QPKEESKPKE 400
    VPSEGRIELC KIDVSKASSQ EIEDALRRPI QKTEQIMELV KPIVDNVRQN 450
    GDKALLELTA KFDGVALKTP VLEAPFPEEL MQLPDNVKRA IDLSIDNVRK 500
    FHEAQLAETL QVETCPGVVC SRFARPIEKV GLYIPGGTAI LPSTSLMLGV 550
    PAKVAGCKEI VFASPPKKDG TLTPEVIYVA HKVGAKCIVL AGGAQAVAAM 600
    AYGTETVPKC DKIFGPGNQF VTAAKMMVQN DTSALCSIDM PAGPSEVLVI 650
    ADKYADPDFV VSDLLSQAEH GIDSQVILLA VDMTDKELAR IEDAVHNQAV 700
    QLPRVEIVRK CIAHSTTLSV ATYEQALEMS NQYAPEHLIL QIENASYVDQ 750
    VQHAGSVFVG AYSPESCGDY SSGTNHTLPT YGYARQYSGV NTATFQKFIT 800
    SQDVTPEGLK HIGQAVMDLA AVEGLDAHRN AVKVRMEKLG LI 842
    Length:842
    Mass (Da):91,942
    Last modified:November 1, 1995 - v1
    Checksum:i67DA24E2266A4A3E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti92 – 921R → RM1 PublicationCurated
    Sequence conflicti507 – 5071A → T in CAA39641. 1 PublicationCurated
    Sequence conflicti661 – 6611V → A in CAA39641. 1 PublicationCurated
    Sequence conflicti746 – 7461S → SS in CAA39641. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14126 Genomic DNA. Translation: AAA67001.1.
    X56180 Genomic DNA. Translation: CAA39641.1.
    PIRiS51513.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14126 Genomic DNA. Translation: AAA67001.1 .
    X56180 Genomic DNA. Translation: CAA39641.1 .
    PIRi S51513.

    3D structure databases

    ProteinModelPortali P45353.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P45353.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00031 ; UER00007 .
    UPA00031 ; UER00008 .
    UPA00031 ; UER00014 .

    Family and domain databases

    HAMAPi MF_01024. HisD.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016298. Histidine_synth_trifunct.
    IPR001692. Histidinol_DH_CS.
    IPR012131. Hstdl_DH.
    IPR008179. PRib-ATP_PPHydrolase.
    IPR021130. PRib-ATP_PPHydrolase-like.
    IPR002496. PRib_AMP_CycHydrolase_dom.
    [Graphical view ]
    Pfami PF00815. Histidinol_dh. 1 hit.
    PF01502. PRA-CH. 1 hit.
    PF01503. PRA-PH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001257. His_trifunctional. 1 hit.
    PRINTSi PR00083. HOLDHDRGNASE.
    ProDomi PD002610. PRA_CycHdrlase. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR00069. hisD. 1 hit.
    TIGR03188. histidine_hisI. 1 hit.
    PROSITEi PS00611. HISOL_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a non-reverting his4 deletion mutant, and development of HIS4-based replicating and integrating plasmids."
      Crane D.I., Gould S.J.
      Curr. Genet. 26:443-450(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Koutz P.J., Davis G.R., Thill G.P.
      Submitted (OCT-1990) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NRRL Y-11430.

    Entry informationi

    Entry nameiHIS2_PICPA
    AccessioniPrimary (citable) accession number: P45353
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3