Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P45350

- DRTS_DAUCA

UniProt

P45350 - DRTS_DAUCA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene
N/A
Organism
Daucus carota (Wild carrot)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271Substrate; via carbonyl oxygenBy similarity
Binding sitei29 – 291NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei49 – 491SubstrateBy similarity
Binding sitei136 – 1361Substrate; via carbonyl oxygenBy similarity
Binding sitei142 – 1421SubstrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Binding sitei264 – 2641dUMPBy similarity
Active sitei409 – 4091By similarity
Binding sitei410 – 4101dUMPBy similarity
Binding sitei440 – 4401dUMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 417NADPBy similarity
Nucleotide bindingi73 – 753NADPBy similarity
Nucleotide bindingi94 – 974NADPBy similarity
Nucleotide bindingi137 – 1448NADPBy similarity
Nucleotide bindingi428 – 4325dUMPBy similarity
Nucleotide bindingi470 – 4723dUMPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. glycine biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
OrganismiDaucus carota (Wild carrot)
Taxonomic identifieri4039 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeScandiceaeDaucinaeDaucus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 528528Bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000186357Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP45350.
SMRiP45350. Positions 242-528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 200178DHFRAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 528327Thymidylate synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45350-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASELLANPT NGSGITRPDP QRTYQVVVAA TQNMGIGKDG KLPWRLPSDM
60 70 80 90 100
KFFKDVTMTT SDPLKRNAVI MGRKTWESIP IQHRPLPGRL NVVLTRSGSF
110 120 130 140 150
DIATVENVVI CGSMISALEL LAGSPYCVSV EKVFVIGGGQ IYREALNAPG
160 170 180 190 200
CDAVHITEIE EHIECDTFIP LLDESVFQPW YSSFPLVENK IRYCFTTYVR
210 220 230 240 250
VRNSVAELTS QTNGCSSDSK SDSGNFEIQN FSFLPKTVFE KHEEYLYLGL
260 270 280 290 300
VENIISNGVT KNDRTRTGTV SIFGCQMRFN LRKSFPLLTT KKVFWRGVVE
310 320 330 340 350
ELLWFISGST NAKILKEKGV NIWEGNGSRE YLDSIGLTDR EEGDLGPIYG
360 370 380 390 400
FQWRHFGARY TDMHADYSGQ GFDQLLDVIS KIKNNPDDRR IIQSAWNPSD
410 420 430 440 450
LRLMALPPCH MFAQFYVANG ELSCQMYQRS ADMGLGVPFN IAAYALLTCM
460 470 480 490 500
IAHVCDLVPG DFVHSIGDAH VYSNHLSDLF ETSFRMLPKT FPVLKINSGE
510 520
KDIDSFEAAD FKLIGYDPHQ KIEMKMAV
Length:528
Mass (Da):59,259
Last modified:November 1, 1995 - v1
Checksum:i8BE07C221A546A56
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z17306 mRNA. Translation: CAA78954.1.
PIRiS35272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z17306 mRNA. Translation: CAA78954.1 .
PIRi S35272.

3D structure databases

ProteinModelPortali P45350.
SMRi P45350. Positions 242-528.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF000389. DHFR-TS. 1 hit.
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota."
    Luo M., Piffanelli P., Rastelli L., Cella R.
    Plant Mol. Biol. 22:427-435(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Lunga di Amsterdam.

Entry informationi

Entry nameiDRTS_DAUCA
AccessioniPrimary (citable) accession number: P45350
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 1, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3