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Protein

Cyclic pyranopterin monophosphate synthase

Gene

moaA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).UniRule annotation

Catalytic activityi

GTP = cyclic pyranopterin phosphate + diphosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261GTPUniRule annotation
Metal bindingi33 – 331Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi37 – 371Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Binding sitei39 – 391S-adenosyl-L-methionineUniRule annotation
Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Binding sitei76 – 761GTPUniRule annotation
Binding sitei80 – 801S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei107 – 1071GTPUniRule annotation
Binding sitei131 – 1311S-adenosyl-L-methionineUniRule annotation
Binding sitei168 – 1681GTPUniRule annotation
Binding sitei202 – 2021S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi265 – 2651Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation
Metal bindingi268 – 2681Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation
Metal bindingi282 – 2821Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. cyclic pyranopterin monophosphate synthase activity Source: UniProtKB-EC
  3. GTP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic pyranopterin monophosphate synthaseUniRule annotation (EC:4.1.99.18UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:HI_1676
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. molybdopterin synthase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Cyclic pyranopterin monophosphate synthasePRO_0000152966Add
BLAST

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi71421.HI1676.

Structurei

3D structure databases

ProteinModelPortaliP45311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni270 – 2723GTP bindingUniRule annotation

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2896.
KOiK03639.
OMAiGWIHQIR.
OrthoDBiEOG64XXNN.
PhylomeDBiP45311.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSIPIKNVG ESRLVDPFQR QYYYLRLSIT DQCNFRCTYC LPDGYQPEAN
60 70 80 90 100
KPSFLTLKEI THLAQAFAEM GTEKIRLTGG EPTLRKDFIS IAESITNIDG
110 120 130 140 150
IRQLAVTTNG YRMAKDVADW KKAGITSINV SVDSLDPKMF HQITGINKFD
160 170 180 190 200
DVMRGIDRAF EVGYNKVKVN SVLMKNLNDK EFEQFLAWVK DRPIQMRFIE
210 220 230 240 250
LMQTGEMDSF FDKFHLSGQV LADKLLKNGW TLQHKSHTDG PAKVFTHPDY
260 270 280 290 300
AGEIGLIMPY EKNFCASCNR LRVSAKGKLH LCLFGEEGIE LRDLLQSHEQ
310 320 330
QGILQARIFA ALQGKREHHY LHIGDTGVRN HLASIGG
Length:337
Mass (Da):38,305
Last modified:November 1, 1995 - v1
Checksum:i1D47939FBBB2D0DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23321.1.
PIRiB64136.
RefSeqiNP_439818.1. NC_000907.1.
WP_005694398.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23321; AAC23321; HI_1676.
GeneIDi950505.
KEGGihin:HI1676.
PATRICi20192103. VBIHaeInf48452_1755.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23321.1.
PIRiB64136.
RefSeqiNP_439818.1. NC_000907.1.
WP_005694398.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP45311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23321; AAC23321; HI_1676.
GeneIDi950505.
KEGGihin:HI1676.
PATRICi20192103. VBIHaeInf48452_1755.

Phylogenomic databases

eggNOGiCOG2896.
KOiK03639.
OMAiGWIHQIR.
OrthoDBiEOG64XXNN.
PhylomeDBiP45311.

Enzyme and pathway databases

UniPathwayiUPA00344.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiMOAA_HAEIN
AccessioniPrimary (citable) accession number: P45311
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 29, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.