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Protein

GTP 3',8-cyclase

Gene

moaA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.UniRule annotation

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26GTPUniRule annotation1
Metal bindingi33Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi37Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei39S-adenosyl-L-methionineUniRule annotation1
Metal bindingi40Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei76GTPUniRule annotation1
Binding sitei80S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei107GTPUniRule annotation1
Binding sitei131S-adenosyl-L-methionineUniRule annotation1
Binding sitei168GTPUniRule annotation1
Binding sitei202S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi265Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi268Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi282Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi270 – 272GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:HI_1676
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001529661 – 337GTP 3',8-cyclaseAdd BLAST337

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi71421.HI1676.

Structurei

3D structure databases

ProteinModelPortaliP45311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CM1. Bacteria.
COG2896. LUCA.
KOiK03639.
OMAiGWIHQIR.
PhylomeDBiP45311.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSIPIKNVG ESRLVDPFQR QYYYLRLSIT DQCNFRCTYC LPDGYQPEAN
60 70 80 90 100
KPSFLTLKEI THLAQAFAEM GTEKIRLTGG EPTLRKDFIS IAESITNIDG
110 120 130 140 150
IRQLAVTTNG YRMAKDVADW KKAGITSINV SVDSLDPKMF HQITGINKFD
160 170 180 190 200
DVMRGIDRAF EVGYNKVKVN SVLMKNLNDK EFEQFLAWVK DRPIQMRFIE
210 220 230 240 250
LMQTGEMDSF FDKFHLSGQV LADKLLKNGW TLQHKSHTDG PAKVFTHPDY
260 270 280 290 300
AGEIGLIMPY EKNFCASCNR LRVSAKGKLH LCLFGEEGIE LRDLLQSHEQ
310 320 330
QGILQARIFA ALQGKREHHY LHIGDTGVRN HLASIGG
Length:337
Mass (Da):38,305
Last modified:November 1, 1995 - v1
Checksum:i1D47939FBBB2D0DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23321.1.
PIRiB64136.
RefSeqiNP_439818.1. NC_000907.1.
WP_005694398.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23321; AAC23321; HI_1676.
GeneIDi950505.
KEGGihin:HI1676.
PATRICi20192103. VBIHaeInf48452_1755.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23321.1.
PIRiB64136.
RefSeqiNP_439818.1. NC_000907.1.
WP_005694398.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP45311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23321; AAC23321; HI_1676.
GeneIDi950505.
KEGGihin:HI1676.
PATRICi20192103. VBIHaeInf48452_1755.

Phylogenomic databases

eggNOGiENOG4105CM1. Bacteria.
COG2896. LUCA.
KOiK03639.
OMAiGWIHQIR.
PhylomeDBiP45311.

Enzyme and pathway databases

UniPathwayiUPA00344.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOAA_HAEIN
AccessioniPrimary (citable) accession number: P45311
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.