Reviewed, UniProtKB/Swiss-Prot P45295 (LDHD_HAEIN)

Names and origin

Protein names

Recommended name:
    D-lactate dehydrogenase
    EC=1.1.1.28

Gene names

Name:	dld
Ordered Locus Names:	HI1649

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First component of the membrane-bound D-lactate oxidase, which is believed to play an important role in the energization of the active transport of a variety of sugars and amino acids By similarity.
Catalytic activity	(R)-lactate + NAD(+) = pyruvate + NADH.
Cofactor	FAD.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic sideBy similarity.
Sequence similarities	Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: InterPro transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	peripheral to membrane of membrane fraction Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: InterPro FAD binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	D-lactate dehydrogenase		PRO_0000084389
Regions
	Domain	36 – 207	172	FAD-binding PCMH-type

Sequences

	Sequence		Length	Mass (Da)
	P45295-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D1211B2D3D5C6418	FASTA	564	64,126
	10 20 30 40 50 60 MSVQQLISRL TDIVGSRYII TDPTKTEAYR SGYRFGTGNA LAVVRPATLL EFWNIVKVCV 70 80 90 100 110 120 EHDVIVINQA ANTGLTGGST PDGNDYDRDI VVINTMRIDG IQLINNASQV ICLPGSTLNE 130 140 150 160 170 180 LENQLKPFGR EPHSVIGSSC IGASVIGGIC NNSGGALVQR GPAYTEMALY AQLNEKGELE 190 200 210 220 230 240 LKNHLGIDLG ETPEEILTNL QEKRYQVKDI RQDCGHGHDH YYCNYVRQVD EGSPARFNAD 250 260 270 280 290 300 PARHYEASGC AGKLAVFAVR LDTFPLEKET AVFYIGTNQT SVLSDIRRHM LVNFEVLPIS 310 320 330 340 350 360 GEYIHRDAFD IAAKYGKDTF WVIKKFGTHW LPKLFSLKSN VDRIGKKFFF LPQHLSDKFM 370 380 390 400 410 420 QTVSKFIPEH LPQSLWDYRN KYEHHLIIKM GGKGIQEARE YLESYIADGS KGGYFECNAI 430 440 450 460 470 480 ETQAAMLHRF AVASAAIRYR AIHEKEVEEI VALDVALRRN DQDWFEVLPP EIDNRIISKL 490 500 510 520 530 540 YYGHFMCHVF HQDYIVKKGY DYEELEYEML KLLDKRGAQY PAEHNVGHLY EAKPTLRKFY 550 560 KELDPTNSFN PGIGKTTRKK YWAE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC23296.1.
PIR	I64134.
RefSeq	NP_439791.1.
3D structure databases
HSSP	HSSP built from PDB template 1F0X based on UniProtKB P06149.
ModBase	Search...
Genome annotation databases
GeneID	950490.
GenomeReviews	Gene locus HI1649 in contig L42023_GR.
KEGG	hin:HI1649.
NMPDR	fig|71421.1.peg.1563.
TIGR	HI1649.
Phylogenomic databases
HOGENOM	P45295.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1649-MON.
Family and domain databases
InterPro	IPR016172. D-lactate_DHase_C-sub1. IPR016173. D-lactate_DHase_C-sub2. IPR012256. D_lactate_DHase. IPR016168. FAD-linked_Oxase_FAD-bd_sub2. IPR015409. Lactate_DHase_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.70.610. D-lactate_dehydrogenase_C-sub1. 1 hit. G3DSA:3.30.1370.20. D-lactate_dehydrogenase_C-sub2. 1 hit. G3DSA:3.30.465.20. FAD-linked_oxidase_FAD-bd_sub2. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF09330. Lact-deh-memb. 1 hit. [Graphical view]
PIRSF	PIRSF000101. D-lactate_dh. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_HAEIN

Accession

Primary (citable) accession number: P45295

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)