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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821NucleophileUniRule annotation
Binding sitei109 – 1091L-glutamineUniRule annotation
Active sitei172 – 1721Charge relay systemUniRule annotation
Active sitei174 – 1741Charge relay systemUniRule annotation

GO - Molecular functioni

  1. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamine catabolic process Source: UniProtKB-HAMAP
  2. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:HI_1648
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Pyridoxal 5'-phosphate synthase subunit PdxTPRO_0000135641Add
BLAST

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi71421.HI1648.

Structurei

3D structure databases

ProteinModelPortaliP45294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 523L-glutamine bindingUniRule annotation
Regioni136 – 1372L-glutamine bindingUniRule annotation

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0311.
KOiK08681.
OMAiGMIMLAD.
OrthoDBiEOG66F0BW.
PhylomeDBiP45294.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT.
InterProiIPR029062. Class_I_gatase-like.
IPR021196. PdxT/SNO_CS.
IPR002161. PDXT_SNO_fam.
[Graphical view]
PfamiPF01174. SNO. 1 hit.
[Graphical view]
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiPS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45294-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIGILALQG AFAEHARMLE KLGIESVELR NLKNFQQHYS DLSGLILPGG
60 70 80 90 100
ESTAIGKLLR ELYMLEPIKQ AISSGFPVFG TCAGLILLAK EITSQKESHF
110 120 130 140 150
GTMDIVVERN AYGRQLGSFY TEADCKGVGK IPMTFIRGPI ISSVGKKVNI
160 170 180 190
LATVNNKIVA AQEKNMLVTS FHPELTNNLS LHKYFIDICK VA
Length:192
Mass (Da):21,070
Last modified:July 5, 2005 - v2
Checksum:i52297D26C0EE5579
GO

Sequence cautioni

The sequence AAC23295.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23295.1. Different initiation.
PIRiG64173.
RefSeqiNP_439790.2. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23295; AAC23295; HI_1648.
GeneIDi950854.
KEGGihin:HI1648.
PATRICi20192041. VBIHaeInf48452_1724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23295.1. Different initiation.
PIRiG64173.
RefSeqiNP_439790.2. NC_000907.1.

3D structure databases

ProteinModelPortaliP45294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1648.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23295; AAC23295; HI_1648.
GeneIDi950854.
KEGGihin:HI1648.
PATRICi20192041. VBIHaeInf48452_1724.

Phylogenomic databases

eggNOGiCOG0311.
KOiK08681.
OMAiGMIMLAD.
OrthoDBiEOG66F0BW.
PhylomeDBiP45294.

Enzyme and pathway databases

UniPathwayiUPA00245.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT.
InterProiIPR029062. Class_I_gatase-like.
IPR021196. PdxT/SNO_CS.
IPR002161. PDXT_SNO_fam.
[Graphical view]
PfamiPF01174. SNO. 1 hit.
[Graphical view]
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiPS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiPDXT_HAEIN
AccessioniPrimary (citable) accession number: P45294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 5, 2005
Last modified: February 4, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.