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P45274

- AMPN_HAEIN

UniProt

P45274 - AMPN_HAEIN

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Protein
Aminopeptidase N
Gene
pepN, HI_1614
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation By similarity.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221Substrate By similarity
Metal bindingi298 – 2981Zinc; catalytic By similarity
Active sitei299 – 2991Proton acceptor By similarity
Metal bindingi302 – 3021Zinc; catalytic By similarity
Metal bindingi321 – 3211Zinc; catalytic By similarity
Sitei382 – 3821Transition state stabilizer By similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alpha-aminoacylpeptide hydrolase
Gene namesi
Name:pepN
Ordered Locus Names:HI_1614
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Aminopeptidase N
PRO_0000095070Add
BLAST

Proteomic databases

PRIDEiP45274.

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1614.

Structurei

3D structure databases

ProteinModelPortaliP45274.
SMRiP45274. Positions 4-868.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni262 – 2665Substrate binding By similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.

Phylogenomic databases

eggNOGiCOG0308.
KOiK01256.
OMAiRWDAAQM.
OrthoDBiEOG693GJ0.
PhylomeDBiP45274.

Family and domain databases

Gene3Di1.25.50.10. 1 hit.
InterProiIPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR012779. Peptidase_M1_pepN.
IPR024601. Peptidase_M1_pepN_C.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11940. DUF3458. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
TIGRFAMsiTIGR02414. pepN_proteo. 1 hit.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45274-1 [UniParc]FASTAAdd to Basket

« Hide

MLAKAKYRKD YKQPDFTVTD IYLDFQLDPK HTVVTAITKF QRLNNEATSL    50
CLDGHSFQFS SIKFNGEPFS DYQQDGESLT LDLKDKSADE FEIEIVTFLV 100
PAENTSLQGL YQSGEGICTQ CEAEGFRQIT YMLDRPDVLA RYITKITADK 150
TKYPFLLSNG NRIASGELED GRHWVEWNDP FPKPSYLFAL VAGDFDLLQD 200
KFITKSGREV ALELYVDRGN LNRATWAMES LKKAMKWDED RFNLEYDLDI 250
YMIVAVDFFN MGAMENKGLN IFNSKFVLAN PQTATDDDYL AIESVIAHEY 300
FHNWTGNRVT CRDWFQLSLK EGLTVFRDQE FSSDTGSRAV NRINNVKFLR 350
TVQFAEDASP MSHPIRPEKV IEMNNFYTVT VYEKGAEVIR MLHTLLGEQG 400
FQKGMQLYIA ENDGKAATCE DFVSAMERAN NLDLNQFRRW YSQSGTPELL 450
ISDAYDEKTH TYRLTVSQST PPTADQMEKV NLHIPLKVAL YDANGTKQML 500
QHNGELLSDV LNVTEKDQVF EFHGIYGRPI PALLCDFSAP VKLDYDYKTE 550
QLLGLLKFAD NQFIRWDAAQ MLFAQELRRN VVRFQQGEAL EISPEILTAL 600
SYVLNHYEKD IELATLILTL PKEMEFAESF KTIDPDGISA ARAFMQAQIA 650
ESLKDDFLRV YTHIRLNDYQ VTQQDIALRV MRNLCLTYLA YTNLGNNLVQ 700
KHYNNANNMT DTLAALSVAT KAALLCRDVL LADFEQKWQH DGLVMDKWFA 750
LQATRPDDNV LEIIQLLMDH PSFNFNNPNR LRSLVGSFAN HNLKAFHNVS 800
GSGYRFLTDV LIRLNESNPQ VAARLIEPLI RFSRFDAQRQ TLMKRALERL 850
SVVENLSKDL FEKIEKALQ 869
Length:869
Mass (Da):99,786
Last modified:November 1, 1995 - v1
Checksum:iA00381DC6FB725D1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581Q → G1 Publication
Sequence conflicti93 – 931I → L1 Publication
Sequence conflicti121 – 1211C → T1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC23262.1.
Z33502 Genomic DNA. Translation: CAA83910.1.
U58657 Genomic DNA. Translation: AAB70877.1.
PIRiF64132.
RefSeqiNP_439756.1. NC_000907.1.
WP_005693625.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23262; AAC23262; HI_1614.
GeneIDi950613.
KEGGihin:HI1614.
PATRICi20191959. VBIHaeInf48452_1687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC23262.1 .
Z33502 Genomic DNA. Translation: CAA83910.1 .
U58657 Genomic DNA. Translation: AAB70877.1 .
PIRi F64132.
RefSeqi NP_439756.1. NC_000907.1.
WP_005693625.1. NC_000907.1.

3D structure databases

ProteinModelPortali P45274.
SMRi P45274. Positions 4-868.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI1614.

Protein family/group databases

MEROPSi M01.005.

Proteomic databases

PRIDEi P45274.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC23262 ; AAC23262 ; HI_1614 .
GeneIDi 950613.
KEGGi hin:HI1614.
PATRICi 20191959. VBIHaeInf48452_1687.

Phylogenomic databases

eggNOGi COG0308.
KOi K01256.
OMAi RWDAAQM.
OrthoDBi EOG693GJ0.
PhylomeDBi P45274.

Family and domain databases

Gene3Di 1.25.50.10. 1 hit.
InterProi IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR012779. Peptidase_M1_pepN.
IPR024601. Peptidase_M1_pepN_C.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11940. DUF3458. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
TIGRFAMsi TIGR02414. pepN_proteo. 1 hit.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. "Identification of hifD and hifE in the pilus gene cluster of Haemophilus influenzae type b strain Eagan."
    McCrea K.W., Watson W.J., Gilsdorf J.R., Marrs C.F.
    Infect. Immun. 62:4922-4928(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-129.
    Strain: Eagan / Serotype B.
  3. "The fimbrial gene cluster of Haemophilus influenzae type b."
    van Ham M.S., van Alphen L., Mooi F.R., van Putten J.P.M.
    Mol. Microbiol. 13:673-684(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
    Strain: AM30 (770235) / Serotype B.

Entry informationi

Entry nameiAMPN_HAEIN
AccessioniPrimary (citable) accession number: P45274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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