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P45274

- AMPN_HAEIN

UniProt

P45274 - AMPN_HAEIN

Protein

Aminopeptidase N

Gene

pepN

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation.By similarity

    Catalytic activityi

    Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei122 – 1221SubstrateBy similarity
    Metal bindingi298 – 2981Zinc; catalyticPROSITE-ProRule annotation
    Active sitei299 – 2991Proton acceptorPROSITE-ProRule annotation
    Metal bindingi302 – 3021Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi321 – 3211Zinc; catalyticPROSITE-ProRule annotation
    Sitei382 – 3821Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW
    2. metallopeptidase activity Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM01.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase N (EC:3.4.11.2)
    Alternative name(s):
    Alpha-aminoacylpeptide hydrolase
    Gene namesi
    Name:pepN
    Ordered Locus Names:HI_1614
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Cell inner membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 869869Aminopeptidase NPRO_0000095070Add
    BLAST

    Proteomic databases

    PRIDEiP45274.

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI1614.

    Structurei

    3D structure databases

    ProteinModelPortaliP45274.
    SMRiP45274. Positions 4-868.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni262 – 2665Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0308.
    KOiK01256.
    OMAiRWDAAQM.
    OrthoDBiEOG693GJ0.
    PhylomeDBiP45274.

    Family and domain databases

    Gene3Di1.25.50.10. 1 hit.
    InterProiIPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    IPR012779. Peptidase_M1_pepN.
    IPR024601. Peptidase_M1_pepN_C.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PfamiPF11940. DUF3458. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    TIGRFAMsiTIGR02414. pepN_proteo. 1 hit.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45274-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLAKAKYRKD YKQPDFTVTD IYLDFQLDPK HTVVTAITKF QRLNNEATSL    50
    CLDGHSFQFS SIKFNGEPFS DYQQDGESLT LDLKDKSADE FEIEIVTFLV 100
    PAENTSLQGL YQSGEGICTQ CEAEGFRQIT YMLDRPDVLA RYITKITADK 150
    TKYPFLLSNG NRIASGELED GRHWVEWNDP FPKPSYLFAL VAGDFDLLQD 200
    KFITKSGREV ALELYVDRGN LNRATWAMES LKKAMKWDED RFNLEYDLDI 250
    YMIVAVDFFN MGAMENKGLN IFNSKFVLAN PQTATDDDYL AIESVIAHEY 300
    FHNWTGNRVT CRDWFQLSLK EGLTVFRDQE FSSDTGSRAV NRINNVKFLR 350
    TVQFAEDASP MSHPIRPEKV IEMNNFYTVT VYEKGAEVIR MLHTLLGEQG 400
    FQKGMQLYIA ENDGKAATCE DFVSAMERAN NLDLNQFRRW YSQSGTPELL 450
    ISDAYDEKTH TYRLTVSQST PPTADQMEKV NLHIPLKVAL YDANGTKQML 500
    QHNGELLSDV LNVTEKDQVF EFHGIYGRPI PALLCDFSAP VKLDYDYKTE 550
    QLLGLLKFAD NQFIRWDAAQ MLFAQELRRN VVRFQQGEAL EISPEILTAL 600
    SYVLNHYEKD IELATLILTL PKEMEFAESF KTIDPDGISA ARAFMQAQIA 650
    ESLKDDFLRV YTHIRLNDYQ VTQQDIALRV MRNLCLTYLA YTNLGNNLVQ 700
    KHYNNANNMT DTLAALSVAT KAALLCRDVL LADFEQKWQH DGLVMDKWFA 750
    LQATRPDDNV LEIIQLLMDH PSFNFNNPNR LRSLVGSFAN HNLKAFHNVS 800
    GSGYRFLTDV LIRLNESNPQ VAARLIEPLI RFSRFDAQRQ TLMKRALERL 850
    SVVENLSKDL FEKIEKALQ 869
    Length:869
    Mass (Da):99,786
    Last modified:November 1, 1995 - v1
    Checksum:iA00381DC6FB725D1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti58 – 581Q → G(PubMed:7927773)Curated
    Sequence conflicti93 – 931I → L(PubMed:7927773)Curated
    Sequence conflicti121 – 1211C → T(PubMed:7927773)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC23262.1.
    Z33502 Genomic DNA. Translation: CAA83910.1.
    U58657 Genomic DNA. Translation: AAB70877.1.
    PIRiF64132.
    RefSeqiNP_439756.1. NC_000907.1.
    WP_005693625.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC23262; AAC23262; HI_1614.
    GeneIDi950613.
    KEGGihin:HI1614.
    PATRICi20191959. VBIHaeInf48452_1687.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC23262.1 .
    Z33502 Genomic DNA. Translation: CAA83910.1 .
    U58657 Genomic DNA. Translation: AAB70877.1 .
    PIRi F64132.
    RefSeqi NP_439756.1. NC_000907.1.
    WP_005693625.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P45274.
    SMRi P45274. Positions 4-868.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI1614.

    Protein family/group databases

    MEROPSi M01.005.

    Proteomic databases

    PRIDEi P45274.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC23262 ; AAC23262 ; HI_1614 .
    GeneIDi 950613.
    KEGGi hin:HI1614.
    PATRICi 20191959. VBIHaeInf48452_1687.

    Phylogenomic databases

    eggNOGi COG0308.
    KOi K01256.
    OMAi RWDAAQM.
    OrthoDBi EOG693GJ0.
    PhylomeDBi P45274.

    Family and domain databases

    Gene3Di 1.25.50.10. 1 hit.
    InterProi IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    IPR012779. Peptidase_M1_pepN.
    IPR024601. Peptidase_M1_pepN_C.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    Pfami PF11940. DUF3458. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    TIGRFAMsi TIGR02414. pepN_proteo. 1 hit.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. "Identification of hifD and hifE in the pilus gene cluster of Haemophilus influenzae type b strain Eagan."
      McCrea K.W., Watson W.J., Gilsdorf J.R., Marrs C.F.
      Infect. Immun. 62:4922-4928(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-129.
      Strain: Eagan / Serotype B.
    3. "The fimbrial gene cluster of Haemophilus influenzae type b."
      van Ham M.S., van Alphen L., Mooi F.R., van Putten J.P.M.
      Mol. Microbiol. 13:673-684(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
      Strain: AM30 (770235) / Serotype B.

    Entry informationi

    Entry nameiAMPN_HAEIN
    AccessioniPrimary (citable) accession number: P45274
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3