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P45274

- AMPN_HAEIN

UniProt

P45274 - AMPN_HAEIN

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Protein

Aminopeptidase N

Gene

pepN

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation.By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221SubstrateBy similarity
Metal bindingi298 – 2981Zinc; catalyticPROSITE-ProRule annotation
Active sitei299 – 2991Proton acceptorPROSITE-ProRule annotation
Metal bindingi302 – 3021Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi321 – 3211Zinc; catalyticPROSITE-ProRule annotation
Sitei382 – 3821Transition state stabilizerBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alpha-aminoacylpeptide hydrolase
Gene namesi
Name:pepN
Ordered Locus Names:HI_1614
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cell inner membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Aminopeptidase NPRO_0000095070Add
BLAST

Proteomic databases

PRIDEiP45274.

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1614.

Structurei

3D structure databases

ProteinModelPortaliP45274.
SMRiP45274. Positions 4-868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni262 – 2665Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiCOG0308.
KOiK01256.
OMAiRWDAAQM.
OrthoDBiEOG693GJ0.
PhylomeDBiP45274.

Family and domain databases

Gene3Di1.25.50.10. 1 hit.
InterProiIPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR012779. Peptidase_M1_pepN.
IPR024601. Peptidase_M1_pepN_C.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11940. DUF3458. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
TIGRFAMsiTIGR02414. pepN_proteo. 1 hit.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45274-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLAKAKYRKD YKQPDFTVTD IYLDFQLDPK HTVVTAITKF QRLNNEATSL
60 70 80 90 100
CLDGHSFQFS SIKFNGEPFS DYQQDGESLT LDLKDKSADE FEIEIVTFLV
110 120 130 140 150
PAENTSLQGL YQSGEGICTQ CEAEGFRQIT YMLDRPDVLA RYITKITADK
160 170 180 190 200
TKYPFLLSNG NRIASGELED GRHWVEWNDP FPKPSYLFAL VAGDFDLLQD
210 220 230 240 250
KFITKSGREV ALELYVDRGN LNRATWAMES LKKAMKWDED RFNLEYDLDI
260 270 280 290 300
YMIVAVDFFN MGAMENKGLN IFNSKFVLAN PQTATDDDYL AIESVIAHEY
310 320 330 340 350
FHNWTGNRVT CRDWFQLSLK EGLTVFRDQE FSSDTGSRAV NRINNVKFLR
360 370 380 390 400
TVQFAEDASP MSHPIRPEKV IEMNNFYTVT VYEKGAEVIR MLHTLLGEQG
410 420 430 440 450
FQKGMQLYIA ENDGKAATCE DFVSAMERAN NLDLNQFRRW YSQSGTPELL
460 470 480 490 500
ISDAYDEKTH TYRLTVSQST PPTADQMEKV NLHIPLKVAL YDANGTKQML
510 520 530 540 550
QHNGELLSDV LNVTEKDQVF EFHGIYGRPI PALLCDFSAP VKLDYDYKTE
560 570 580 590 600
QLLGLLKFAD NQFIRWDAAQ MLFAQELRRN VVRFQQGEAL EISPEILTAL
610 620 630 640 650
SYVLNHYEKD IELATLILTL PKEMEFAESF KTIDPDGISA ARAFMQAQIA
660 670 680 690 700
ESLKDDFLRV YTHIRLNDYQ VTQQDIALRV MRNLCLTYLA YTNLGNNLVQ
710 720 730 740 750
KHYNNANNMT DTLAALSVAT KAALLCRDVL LADFEQKWQH DGLVMDKWFA
760 770 780 790 800
LQATRPDDNV LEIIQLLMDH PSFNFNNPNR LRSLVGSFAN HNLKAFHNVS
810 820 830 840 850
GSGYRFLTDV LIRLNESNPQ VAARLIEPLI RFSRFDAQRQ TLMKRALERL
860
SVVENLSKDL FEKIEKALQ
Length:869
Mass (Da):99,786
Last modified:November 1, 1995 - v1
Checksum:iA00381DC6FB725D1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581Q → G(PubMed:7927773)Curated
Sequence conflicti93 – 931I → L(PubMed:7927773)Curated
Sequence conflicti121 – 1211C → T(PubMed:7927773)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23262.1.
Z33502 Genomic DNA. Translation: CAA83910.1.
U58657 Genomic DNA. Translation: AAB70877.1.
PIRiF64132.
RefSeqiNP_439756.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23262; AAC23262; HI_1614.
GeneIDi950613.
KEGGihin:HI1614.
PATRICi20191959. VBIHaeInf48452_1687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23262.1 .
Z33502 Genomic DNA. Translation: CAA83910.1 .
U58657 Genomic DNA. Translation: AAB70877.1 .
PIRi F64132.
RefSeqi NP_439756.1. NC_000907.1.

3D structure databases

ProteinModelPortali P45274.
SMRi P45274. Positions 4-868.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI1614.

Protein family/group databases

MEROPSi M01.005.

Proteomic databases

PRIDEi P45274.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC23262 ; AAC23262 ; HI_1614 .
GeneIDi 950613.
KEGGi hin:HI1614.
PATRICi 20191959. VBIHaeInf48452_1687.

Phylogenomic databases

eggNOGi COG0308.
KOi K01256.
OMAi RWDAAQM.
OrthoDBi EOG693GJ0.
PhylomeDBi P45274.

Family and domain databases

Gene3Di 1.25.50.10. 1 hit.
InterProi IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR012779. Peptidase_M1_pepN.
IPR024601. Peptidase_M1_pepN_C.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11940. DUF3458. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
TIGRFAMsi TIGR02414. pepN_proteo. 1 hit.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. "Identification of hifD and hifE in the pilus gene cluster of Haemophilus influenzae type b strain Eagan."
    McCrea K.W., Watson W.J., Gilsdorf J.R., Marrs C.F.
    Infect. Immun. 62:4922-4928(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-129.
    Strain: Eagan / Serotype B.
  3. "The fimbrial gene cluster of Haemophilus influenzae type b."
    van Ham M.S., van Alphen L., Mooi F.R., van Putten J.P.M.
    Mol. Microbiol. 13:673-684(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
    Strain: AM30 (770235) / Serotype B.

Entry informationi

Entry nameiAMPN_HAEIN
AccessioniPrimary (citable) accession number: P45274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3