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P45274 (AMPN_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aminopeptidase N

EC=3.4.11.2
Alternative name(s):
Alpha-aminoacylpeptide hydrolase
Gene names
Name:pepN
Ordered Locus Names:HI_1614
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length869 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation By similarity.

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.

Sequence similarities

Belongs to the peptidase M1 family.

Ontologies

Keywords
   Cellular componentCell inner membrane
Cell membrane
Membrane
   LigandMetal-binding
Zinc
   Molecular functionAminopeptidase
Hydrolase
Metalloprotease
Protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metallopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 869869Aminopeptidase N
PRO_0000095070

Regions

Region262 – 2665Substrate binding By similarity

Sites

Active site2991Proton acceptor By similarity
Metal binding2981Zinc; catalytic By similarity
Metal binding3021Zinc; catalytic By similarity
Metal binding3211Zinc; catalytic By similarity
Binding site1221Substrate By similarity
Site3821Transition state stabilizer By similarity

Experimental info

Sequence conflict581Q → G Ref.2
Sequence conflict931I → L Ref.2
Sequence conflict1211C → T Ref.2

Sequences

Sequence LengthMass (Da)Tools
P45274 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A00381DC6FB725D1

FASTA86999,786
        10         20         30         40         50         60 
MLAKAKYRKD YKQPDFTVTD IYLDFQLDPK HTVVTAITKF QRLNNEATSL CLDGHSFQFS 

        70         80         90        100        110        120 
SIKFNGEPFS DYQQDGESLT LDLKDKSADE FEIEIVTFLV PAENTSLQGL YQSGEGICTQ 

       130        140        150        160        170        180 
CEAEGFRQIT YMLDRPDVLA RYITKITADK TKYPFLLSNG NRIASGELED GRHWVEWNDP 

       190        200        210        220        230        240 
FPKPSYLFAL VAGDFDLLQD KFITKSGREV ALELYVDRGN LNRATWAMES LKKAMKWDED 

       250        260        270        280        290        300 
RFNLEYDLDI YMIVAVDFFN MGAMENKGLN IFNSKFVLAN PQTATDDDYL AIESVIAHEY 

       310        320        330        340        350        360 
FHNWTGNRVT CRDWFQLSLK EGLTVFRDQE FSSDTGSRAV NRINNVKFLR TVQFAEDASP 

       370        380        390        400        410        420 
MSHPIRPEKV IEMNNFYTVT VYEKGAEVIR MLHTLLGEQG FQKGMQLYIA ENDGKAATCE 

       430        440        450        460        470        480 
DFVSAMERAN NLDLNQFRRW YSQSGTPELL ISDAYDEKTH TYRLTVSQST PPTADQMEKV 

       490        500        510        520        530        540 
NLHIPLKVAL YDANGTKQML QHNGELLSDV LNVTEKDQVF EFHGIYGRPI PALLCDFSAP 

       550        560        570        580        590        600 
VKLDYDYKTE QLLGLLKFAD NQFIRWDAAQ MLFAQELRRN VVRFQQGEAL EISPEILTAL 

       610        620        630        640        650        660 
SYVLNHYEKD IELATLILTL PKEMEFAESF KTIDPDGISA ARAFMQAQIA ESLKDDFLRV 

       670        680        690        700        710        720 
YTHIRLNDYQ VTQQDIALRV MRNLCLTYLA YTNLGNNLVQ KHYNNANNMT DTLAALSVAT 

       730        740        750        760        770        780 
KAALLCRDVL LADFEQKWQH DGLVMDKWFA LQATRPDDNV LEIIQLLMDH PSFNFNNPNR 

       790        800        810        820        830        840 
LRSLVGSFAN HNLKAFHNVS GSGYRFLTDV LIRLNESNPQ VAARLIEPLI RFSRFDAQRQ 

       850        860 
TLMKRALERL SVVENLSKDL FEKIEKALQ 

« Hide

References

« Hide 'large scale' references
[1]"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd."
Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. expand/collapse author list , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C.
Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]"Identification of hifD and hifE in the pilus gene cluster of Haemophilus influenzae type b strain Eagan."
McCrea K.W., Watson W.J., Gilsdorf J.R., Marrs C.F.
Infect. Immun. 62:4922-4928(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-129.
Strain: Eagan / Serotype B.
[3]"The fimbrial gene cluster of Haemophilus influenzae type b."
van Ham M.S., van Alphen L., Mooi F.R., van Putten J.P.M.
Mol. Microbiol. 13:673-684(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
Strain: AM30 (770235) / Serotype B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC23262.1.
Z33502 Genomic DNA. Translation: CAA83910.1.
U58657 Genomic DNA. Translation: AAB70877.1.
PIRF64132.
RefSeqNP_439756.1. NC_000907.1.

3D structure databases

ProteinModelPortalP45274.
SMRP45274. Positions 4-868.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI1614.

Protein family/group databases

MEROPSM01.005.

Proteomic databases

PRIDEP45274.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC23262; AAC23262; HI_1614.
GeneID950613.
KEGGhin:HI1614.
PATRIC20191959. VBIHaeInf48452_1687.

Phylogenomic databases

eggNOGCOG0308.
KOK01256.
OMADIFMIVA.
OrthoDBEOG693GJ0.
ProtClustDBPRK14015.

Family and domain databases

Gene3D1.25.50.10. 1 hit.
InterProIPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR012779. Peptidase_M1_pepN.
IPR024601. Peptidase_M1_pepN_C.
[Graphical view]
PANTHERPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF8. PTHR11533:SF8. 1 hit.
PfamPF11940. DUF3458. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSPR00756. ALADIPTASE.
TIGRFAMsTIGR02414. pepN_proteo. 1 hit.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPN_HAEIN
AccessionPrimary (citable) accession number: P45274
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 13, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names