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Protein

Aminopeptidase N

Gene

pepN

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation.By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122SubstrateBy similarity1
Metal bindingi298Zinc; catalyticPROSITE-ProRule annotation1
Active sitei299Proton acceptorPROSITE-ProRule annotation1
Metal bindingi302Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi321Zinc; catalyticPROSITE-ProRule annotation1
Sitei382Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alpha-aminoacylpeptide hydrolase
Gene namesi
Name:pepN
Ordered Locus Names:HI_1614
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950701 – 869Aminopeptidase NAdd BLAST869

Proteomic databases

PRIDEiP45274.

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1614.

Structurei

3D structure databases

ProteinModelPortaliP45274.
SMRiP45274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 266Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiENOG4105CD8. Bacteria.
COG0308. LUCA.
KOiK01256.
OMAiFKRWYSQ.
PhylomeDBiP45274.

Family and domain databases

CDDicd09600. M1_APN_1. 1 hit.
Gene3Di1.25.50.10. 1 hit.
InterProiView protein in InterPro
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
IPR012779. Peptidase_M1_pepN.
IPR024601. Peptidase_M1_pepN_C.
IPR035414. Peptidase_M1_pepN_Ig-like.
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiView protein in Pfam
PF11940. DUF3458. 1 hit.
PF17432. DUF3458_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
PRINTSiPR00756. ALADIPTASE.
TIGRFAMsiTIGR02414. pepN_proteo. 1 hit.
PROSITEiView protein in PROSITE
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P45274-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAKAKYRKD YKQPDFTVTD IYLDFQLDPK HTVVTAITKF QRLNNEATSL
60 70 80 90 100
CLDGHSFQFS SIKFNGEPFS DYQQDGESLT LDLKDKSADE FEIEIVTFLV
110 120 130 140 150
PAENTSLQGL YQSGEGICTQ CEAEGFRQIT YMLDRPDVLA RYITKITADK
160 170 180 190 200
TKYPFLLSNG NRIASGELED GRHWVEWNDP FPKPSYLFAL VAGDFDLLQD
210 220 230 240 250
KFITKSGREV ALELYVDRGN LNRATWAMES LKKAMKWDED RFNLEYDLDI
260 270 280 290 300
YMIVAVDFFN MGAMENKGLN IFNSKFVLAN PQTATDDDYL AIESVIAHEY
310 320 330 340 350
FHNWTGNRVT CRDWFQLSLK EGLTVFRDQE FSSDTGSRAV NRINNVKFLR
360 370 380 390 400
TVQFAEDASP MSHPIRPEKV IEMNNFYTVT VYEKGAEVIR MLHTLLGEQG
410 420 430 440 450
FQKGMQLYIA ENDGKAATCE DFVSAMERAN NLDLNQFRRW YSQSGTPELL
460 470 480 490 500
ISDAYDEKTH TYRLTVSQST PPTADQMEKV NLHIPLKVAL YDANGTKQML
510 520 530 540 550
QHNGELLSDV LNVTEKDQVF EFHGIYGRPI PALLCDFSAP VKLDYDYKTE
560 570 580 590 600
QLLGLLKFAD NQFIRWDAAQ MLFAQELRRN VVRFQQGEAL EISPEILTAL
610 620 630 640 650
SYVLNHYEKD IELATLILTL PKEMEFAESF KTIDPDGISA ARAFMQAQIA
660 670 680 690 700
ESLKDDFLRV YTHIRLNDYQ VTQQDIALRV MRNLCLTYLA YTNLGNNLVQ
710 720 730 740 750
KHYNNANNMT DTLAALSVAT KAALLCRDVL LADFEQKWQH DGLVMDKWFA
760 770 780 790 800
LQATRPDDNV LEIIQLLMDH PSFNFNNPNR LRSLVGSFAN HNLKAFHNVS
810 820 830 840 850
GSGYRFLTDV LIRLNESNPQ VAARLIEPLI RFSRFDAQRQ TLMKRALERL
860
SVVENLSKDL FEKIEKALQ
Length:869
Mass (Da):99,786
Last modified:November 1, 1995 - v1
Checksum:iA00381DC6FB725D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58Q → G (PubMed:7927773).Curated1
Sequence conflicti93I → L (PubMed:7927773).Curated1
Sequence conflicti121C → T (PubMed:7927773).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23262.1.
Z33502 Genomic DNA. Translation: CAA83910.1.
U58657 Genomic DNA. Translation: AAB70877.1.
PIRiF64132.
RefSeqiNP_439756.1. NC_000907.1.
WP_005693625.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23262; AAC23262; HI_1614.
GeneIDi950613.
KEGGihin:HI1614.
PATRICifig|71421.8.peg.1687.

Similar proteinsi

Entry informationi

Entry nameiAMPN_HAEIN
AccessioniPrimary (citable) accession number: P45274
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: August 30, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families