Reviewed, UniProtKB/Swiss-Prot P45208 (METF_HAEIN)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	HI1444

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	5,10-methylenetetrahydrofolate reductase		PRO_0000190263

Sequences

	Sequence		Length	Mass (Da)
	P45208-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C6DCC9BC80CFF9B4	FASTA	292	33,020
	10 20 30 40 50 60 MSYAKEIDTL NQHIADFNKK INVSFEFFPP KNEKMETLLW DSIHRLKVLK PKFVSVTYGA 70 80 90 100 110 120 NSGERDRTHG IVKAIKQETG LEAAPHLTGI DATPEELKQI ARDYWDSGIR RIVALRGDEP 130 140 150 160 170 180 KGYAKKPFYA SDLVELLRSV ADFDISVAAY PEVHPEAKSA QADLINLKRK IDAGANHVIT 190 200 210 220 230 240 QFFFDIENYL RFRDRCASIG IDTEIVPGIL PVTNFKQLQK MASFTNVKIP AWLVKAYDGL 250 260 270 280 290 DNDPTTRNLV AASVAMDMVK ILSREGVNDF HFYTLNRSEL TYAICHMLGV RP

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC23094.1.
PIR	H64123.
RefSeq	NP_439596.1.
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
SMR	P45208. Positions 3-292.
ModBase	Search...
Genome annotation databases
GeneID	950344.
GenomeReviews	Gene locus HI1444 in contig L42023_GR.
KEGG	hin:HI1444.
NMPDR	fig|71421.1.peg.1378.
TIGR	HI1444.
Phylogenomic databases
HOGENOM	P45208.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1444-MON.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_HAEIN

Accession

Primary (citable) accession number: P45208

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)