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P45208

- METF_HAEIN

UniProt

P45208 - METF_HAEIN

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei26 – 261Proton donor/acceptorBy similarity
    Binding sitei86 – 861FADBy similarity
    Binding sitei118 – 1181SubstrateBy similarity
    Binding sitei150 – 1501FADBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Binding sitei217 – 2171SubstrateBy similarity
    Binding sitei273 – 2731SubstrateBy similarity
    Binding sitei277 – 2771SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 316NADBy similarity
    Nucleotide bindingi57 – 604FADBy similarity
    Nucleotide bindingi57 – 582NADBy similarity
    Nucleotide bindingi116 – 1183FADBy similarity
    Nucleotide bindingi129 – 1302FADBy similarity
    Nucleotide bindingi154 – 1574FADBy similarity
    Nucleotide bindingi163 – 1708FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    Ordered Locus Names:HI_1444
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2922925,10-methylenetetrahydrofolate reductasePRO_0000190263Add
    BLAST

    Proteomic databases

    PRIDEiP45208.

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI1444.

    Structurei

    3D structure databases

    ProteinModelPortaliP45208.
    SMRiP45208. Positions 3-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    KOiK00297.
    OMAiFEFFPTK.
    OrthoDBiEOG6D2KVW.
    PhylomeDBiP45208.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P45208-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYAKEIDTL NQHIADFNKK INVSFEFFPP KNEKMETLLW DSIHRLKVLK    50
    PKFVSVTYGA NSGERDRTHG IVKAIKQETG LEAAPHLTGI DATPEELKQI 100
    ARDYWDSGIR RIVALRGDEP KGYAKKPFYA SDLVELLRSV ADFDISVAAY 150
    PEVHPEAKSA QADLINLKRK IDAGANHVIT QFFFDIENYL RFRDRCASIG 200
    IDTEIVPGIL PVTNFKQLQK MASFTNVKIP AWLVKAYDGL DNDPTTRNLV 250
    AASVAMDMVK ILSREGVNDF HFYTLNRSEL TYAICHMLGV RP 292
    Length:292
    Mass (Da):33,020
    Last modified:November 1, 1995 - v1
    Checksum:iC6DCC9BC80CFF9B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC23094.1.
    PIRiH64123.
    RefSeqiNP_439596.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC23094; AAC23094; HI_1444.
    GeneIDi950344.
    KEGGihin:HI1444.
    PATRICi20191593. VBIHaeInf48452_1506.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC23094.1 .
    PIRi H64123.
    RefSeqi NP_439596.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P45208.
    SMRi P45208. Positions 3-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI1444.

    Proteomic databases

    PRIDEi P45208.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC23094 ; AAC23094 ; HI_1444 .
    GeneIDi 950344.
    KEGGi hin:HI1444.
    PATRICi 20191593. VBIHaeInf48452_1506.

    Phylogenomic databases

    eggNOGi COG0685.
    KOi K00297.
    OMAi FEFFPTK.
    OrthoDBi EOG6D2KVW.
    PhylomeDBi P45208.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiMETF_HAEIN
    AccessioniPrimary (citable) accession number: P45208
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3