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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei26 – 261Proton donor/acceptorBy similarity
Binding sitei86 – 861FADBy similarity
Binding sitei118 – 1181SubstrateBy similarity
Binding sitei150 – 1501FADBy similarity
Binding sitei181 – 1811SubstrateBy similarity
Binding sitei217 – 2171SubstrateBy similarity
Binding sitei273 – 2731SubstrateBy similarity
Binding sitei277 – 2771SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 316NADBy similarity
Nucleotide bindingi57 – 604FADBy similarity
Nucleotide bindingi57 – 582NADBy similarity
Nucleotide bindingi116 – 1183FADBy similarity
Nucleotide bindingi129 – 1302FADBy similarity
Nucleotide bindingi154 – 1574FADBy similarity
Nucleotide bindingi163 – 1708FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:HI_1444
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2922925,10-methylenetetrahydrofolate reductasePRO_0000190263Add
BLAST

Proteomic databases

PRIDEiP45208.

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1444.

Structurei

3D structure databases

ProteinModelPortaliP45208.
SMRiP45208. Positions 3-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
KOiK00297.
OMAiFEFFPTK.
OrthoDBiEOG6D2KVW.
PhylomeDBiP45208.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P45208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYAKEIDTL NQHIADFNKK INVSFEFFPP KNEKMETLLW DSIHRLKVLK
60 70 80 90 100
PKFVSVTYGA NSGERDRTHG IVKAIKQETG LEAAPHLTGI DATPEELKQI
110 120 130 140 150
ARDYWDSGIR RIVALRGDEP KGYAKKPFYA SDLVELLRSV ADFDISVAAY
160 170 180 190 200
PEVHPEAKSA QADLINLKRK IDAGANHVIT QFFFDIENYL RFRDRCASIG
210 220 230 240 250
IDTEIVPGIL PVTNFKQLQK MASFTNVKIP AWLVKAYDGL DNDPTTRNLV
260 270 280 290
AASVAMDMVK ILSREGVNDF HFYTLNRSEL TYAICHMLGV RP
Length:292
Mass (Da):33,020
Last modified:November 1, 1995 - v1
Checksum:iC6DCC9BC80CFF9B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23094.1.
PIRiH64123.
RefSeqiNP_439596.1. NC_000907.1.
WP_005652747.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23094; AAC23094; HI_1444.
GeneIDi950344.
KEGGihin:HI1444.
PATRICi20191593. VBIHaeInf48452_1506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23094.1.
PIRiH64123.
RefSeqiNP_439596.1. NC_000907.1.
WP_005652747.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP45208.
SMRiP45208. Positions 3-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1444.

Proteomic databases

PRIDEiP45208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23094; AAC23094; HI_1444.
GeneIDi950344.
KEGGihin:HI1444.
PATRICi20191593. VBIHaeInf48452_1506.

Phylogenomic databases

eggNOGiCOG0685.
KOiK00297.
OMAiFEFFPTK.
OrthoDBiEOG6D2KVW.
PhylomeDBiP45208.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiMETF_HAEIN
AccessioniPrimary (citable) accession number: P45208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 22, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.