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P45208

- METF_HAEIN

UniProt

P45208 - METF_HAEIN

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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei26 – 261Proton donor/acceptorBy similarity
Binding sitei86 – 861FADBy similarity
Binding sitei118 – 1181SubstrateBy similarity
Binding sitei150 – 1501FADBy similarity
Binding sitei181 – 1811SubstrateBy similarity
Binding sitei217 – 2171SubstrateBy similarity
Binding sitei273 – 2731SubstrateBy similarity
Binding sitei277 – 2771SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 316NADBy similarity
Nucleotide bindingi57 – 604FADBy similarity
Nucleotide bindingi57 – 582NADBy similarity
Nucleotide bindingi116 – 1183FADBy similarity
Nucleotide bindingi129 – 1302FADBy similarity
Nucleotide bindingi154 – 1574FADBy similarity
Nucleotide bindingi163 – 1708FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:HI_1444
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2922925,10-methylenetetrahydrofolate reductasePRO_0000190263Add
BLAST

Proteomic databases

PRIDEiP45208.

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1444.

Structurei

3D structure databases

ProteinModelPortaliP45208.
SMRiP45208. Positions 3-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
KOiK00297.
OMAiFEFFPTK.
OrthoDBiEOG6D2KVW.
PhylomeDBiP45208.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P45208-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYAKEIDTL NQHIADFNKK INVSFEFFPP KNEKMETLLW DSIHRLKVLK
60 70 80 90 100
PKFVSVTYGA NSGERDRTHG IVKAIKQETG LEAAPHLTGI DATPEELKQI
110 120 130 140 150
ARDYWDSGIR RIVALRGDEP KGYAKKPFYA SDLVELLRSV ADFDISVAAY
160 170 180 190 200
PEVHPEAKSA QADLINLKRK IDAGANHVIT QFFFDIENYL RFRDRCASIG
210 220 230 240 250
IDTEIVPGIL PVTNFKQLQK MASFTNVKIP AWLVKAYDGL DNDPTTRNLV
260 270 280 290
AASVAMDMVK ILSREGVNDF HFYTLNRSEL TYAICHMLGV RP
Length:292
Mass (Da):33,020
Last modified:November 1, 1995 - v1
Checksum:iC6DCC9BC80CFF9B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23094.1.
PIRiH64123.
RefSeqiNP_439596.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23094; AAC23094; HI_1444.
GeneIDi950344.
KEGGihin:HI1444.
PATRICi20191593. VBIHaeInf48452_1506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23094.1 .
PIRi H64123.
RefSeqi NP_439596.1. NC_000907.1.

3D structure databases

ProteinModelPortali P45208.
SMRi P45208. Positions 3-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI1444.

Proteomic databases

PRIDEi P45208.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC23094 ; AAC23094 ; HI_1444 .
GeneIDi 950344.
KEGGi hin:HI1444.
PATRICi 20191593. VBIHaeInf48452_1506.

Phylogenomic databases

eggNOGi COG0685.
KOi K00297.
OMAi FEFFPTK.
OrthoDBi EOG6D2KVW.
PhylomeDBi P45208.

Enzyme and pathway databases

UniPathwayi UPA00193 .

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiMETF_HAEIN
AccessioniPrimary (citable) accession number: P45208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3