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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei405 – 4051NucleophileBy similarity
Active sitei458 – 4581Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:HI_1357
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7307301,4-alpha-glucan branching enzyme GlgBPRO_0000188710Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi71421.HI1357.

Structurei

3D structure databases

ProteinModelPortaliP45177.
SMRiP45177. Positions 119-725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
KOiK00700.
OMAiEGHLYKY.
OrthoDBiEOG6JX7GT.
PhylomeDBiP45177.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

P45177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAVTQAII DGFFDASNGD PFATLGMHET EQGIEIRTLL PDANRMVVIE
60 70 80 90 100
RESGKEITEL DCVDERGFFV GVIPNCRQFF AYQLQVFWGN EAQIIEDPYR
110 120 130 140 150
FHPMIDDLEQ WLLSEGSMLR PYEVLGAHFM ECDGVSGVNF RLWAPNARRV
160 170 180 190 200
SIVGDFNYWD GRRHPMRFHS KSGVWELFLP KASLGQLYKF ELIDCHGNLR
210 220 230 240 250
LKADPFAFSS QLRPDTASQV SALPNVVEMT EARKKANQGN QPISIYEVHL
260 270 280 290 300
GSWRRNLENN FWLDYDQIAD ELIPYVKEMG FTHIEFLPLS EFPFDGSWGY
310 320 330 340 350
QPLGLYSPTS RFGSPEAFRR LVKRAHEAGI NVILDWVPGH FPSDTHGLVA
360 370 380 390 400
FDGTALYEHE DPREGYHQDW NTLIYNYGRN EVKNFLSSNA LYWLERFGVD
410 420 430 440 450
GIRVDAVASM IYRDYSRAEG EWIPNQYGGR ENLEAIEFLK HTNWKIHSEM
460 470 480 490 500
AGAISIAEES TSFAGVTHPS ENGGLGFNFK WNMGWMNDTL AYMKLDPIYR
510 520 530 540 550
QYHHNKMTFG MVYQYSENFV LPLSHDEVVH GKYSLLGKMP GDTWQKFANL
560 570 580 590 600
RAYYGYMWGY PGKKLLFMGN EFAQGREWNY EESLDWFLLD ENIGGGWHKG
610 620 630 640 650
VLKLVKDLNQ IYQKNRPLFE LDNSPEGFDW LVVDDAANSV LAFERRSSNG
660 670 680 690 700
ERIIVVSNFT PVPRHNYRIG VNVAGKYEEI LNTDSMYYEG SNVGNFGCVA
710 720 730
SEQIESHGRE NSISVSIPPL ATVYLRLKTK
Length:730
Mass (Da):83,820
Last modified:October 31, 1995 - v1
Checksum:i5B9575317F53769A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23004.1.
PIRiI64118.
RefSeqiNP_439508.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23004; AAC23004; HI_1357.
GeneIDi950275.
KEGGihin:HI1357.
PATRICi20191399. VBIHaeInf48452_1410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23004.1.
PIRiI64118.
RefSeqiNP_439508.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP45177.
SMRiP45177. Positions 119-725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1357.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23004; AAC23004; HI_1357.
GeneIDi950275.
KEGGihin:HI1357.
PATRICi20191399. VBIHaeInf48452_1410.

Phylogenomic databases

eggNOGiCOG0296.
KOiK00700.
OMAiEGHLYKY.
OrthoDBiEOG6JX7GT.
PhylomeDBiP45177.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiGLGB_HAEIN
AccessioniPrimary (citable) accession number: P45177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: October 31, 1995
Last modified: March 3, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.